Expression of the ferric enterobactin receptor (PfeA) of <i>Pseudomonas aeruginosa</i>: involvement of a two‐component regulatory system

Dean, Charles R.; Poole, Keith

doi:10.1111/j.1365-2958.1993.tb01654.x

Cited by 98 publications

(114 citation statements)

References 42 publications

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“…This inner-membrane-spanning protein then activates two ECFsigma factors, PvdS and FpvI, which are required for the transcription of pyoverdine biosynthesis and uptake genes, respectively (Beare et al, 2003;Lamont et al, 2002;Redly & Poole, 2003). Perception of the heterologous Escherichia coli siderophore enterobactin occurs in the periplasm by the sensor kinase PfeS, which activates its cognate response regulator PfeR by phosphorylation such that PfeR becomes able to upregulate the transcription of the outer-membrane receptor gene pfeA (Dean & Poole, 1993;Dean et al, 1996). Pyochelin sensing occurs in the cytoplasm of P. aeruginosa.…”

Section: Introductionmentioning

confidence: 99%

Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa

2007

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In response to iron starvation, Pseudomonas aeruginosa produces the siderophore pyochelin. When secreted to the extracellular environment, pyochelin chelates iron and transports it to the bacterial cytoplasm via its specific outer-membrane receptor FptA and the inner-membrane permease FptX. Exogenously added pyochelin also acts as a signal which induces the expression of the pyochelin biosynthesis and uptake genes by activating PchR, a cytoplasmic regulatory protein of the AraC/XylS family. The importance of ferripyochelin uptake genes in this regulation was evaluated. The fptA and fptX genes were shown to be part of the fptABCX ferripyochelin transport operon, which is conserved in Burkholderia sp. and Rhodospirillum rubrum. The fptB and fptC genes were found to be dispensable for utilization of pyochelin as an iron source, for signalling and for pyochelin production. By contrast, mutations in fptA and fptX not only interfered with pyochelin utilization, but also affected signalling and diminished siderophore production. It is concluded from this that pyochelin-mediated signalling operates to a large extent via the ferripyochelin transport system.

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Section: Introductionmentioning

confidence: 99%

Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa

2007

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“…Additional transcriptional regulators have been identified that can act as positive regulators of siderophore biosynthesis and transport gene expression, all of which are Fur controlled and responsive to the presence of the cognate iron chelate (14,18,23,24,28). The concerted actions of negatively and positively acting regulators in bacterial species capable of utilizing diverse iron sources may ensure that genes encoding specific nutritional iron transport functions are expressed maximally only under appropriate conditions in which the particular iron source is perceived in the environment, thus conserving energy and precursors.…”

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confidence: 99%

Transcriptional Activation of Bordetella Alcaligin Siderophore Genes Requires the AlcR Regulator with Alcaligin as Inducer

2001

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Genetic and biochemical studies have established that Fur and iron mediate repression of Bordetella alcaligin siderophore system (alc) genes under iron-replete nutritional growth conditions. In this study, transcriptional analyses using Bordetella chromosomal alc-lacZ operon fusions determined that maximal alc gene transcriptional activity under iron starvation stress conditions is dependent on the presence of alcaligin siderophore. Mutational analysis and genetic complementation confirmed that alcaligin-responsive transcriptional activation of Bordetella alcaligin system genes is dependent on AlcR, a Fur-regulated AraC-like positive transcriptional regulator encoded within the alcaligin gene cluster. AlcR-mediated transcriptional activation is remarkably sensitive to inducer, occurring at extremely low alcaligin concentrations. This positive autogenous control circuit involving alcaligin siderophore as the inducer for AlcR-mediated transcriptional activation of alcaligin siderophore biosynthesis and transport genes coordinates environmental and intracellular signals for maximal expression of these genes under conditions in which the presence of alcaligin in the environment is perceived.

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“…In the present work, PvdI and FpvAI are called Pvd and FpvA, respectively. FpvA has been well characterized using physiological, immunological, and molecular approaches (see references 11,16,50, and 51, among others). The structures of P. aeruginosa FptA and FpvA have been solved (12,13).…”

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confidence: 99%

Pyoverdine-Mediated Iron Uptake in Pseudomonas aeruginosa : the Tat System Is Required for PvdN but Not for FpvA Transport

Voulhoux¹,

Filloux²,

Schalk

2006

J Bacteriol

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Under iron-limiting conditions, Pseudomonas aeruginosa PAO1 secretes a fluorescent siderophore called pyoverdine (Pvd). After chelating iron, this ferric siderophore is transported back into the cells via the outer membrane receptor FpvA. The Pvd-dependent iron uptake pathway requires several essential genes involved in both the synthesis of Pvd and the uptake of ferric Pvd inside the cell. A previous study describing the global phenotype of a tat-deficient P. aeruginosa strain showed that the defect in Pvd-mediated iron uptake was due to the Tat . Using biochemical and biophysical tools, we showed that despite its predicted Tat signal sequence, FpvA is correctly located in the outer membrane of a tat mutant and is fully functional for all steps of the iron uptake process (ferric Pvd uptake and recycling of Pvd on FpvA after iron release). However, in the tat mutant, no Pvd was produced. This suggested that a key element in the Pvd biogenesis pathway must be exported to the periplasm by the Tat pathway. We located PvdN, a still unknown but essential component in Pvd biogenesis, at the periplasmic side of the cytoplasmic membrane and showed that its export is Tat dependent. Our results further support the idea that a critical step of the Pvd biogenesis pathway involving PvdN occurs at the periplasmic side of the cytoplasmic membrane.

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Expression of the ferric enterobactin receptor (PfeA) of Pseudomonas aeruginosa: involvement of a two‐component regulatory system

Cited by 98 publications

References 42 publications

Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa

Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa

Transcriptional Activation of Bordetella Alcaligin Siderophore Genes Requires the AlcR Regulator with Alcaligin as Inducer

Pyoverdine-Mediated Iron Uptake in Pseudomonas aeruginosa : the Tat System Is Required for PvdN but Not for FpvA Transport

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