Expression of the Galanthus nivalis agglutinin (GNA) gene in transgenic potato plants confers resistance to aphids

Mi, Xiaoxiao; Liu, Xue; Yan, Haolu; Liang, Linhui; Zhou, Xuyang; Yang, Jie; Zhang, Ning

doi:10.1016/j.crvi.2016.10.003

Cited by 21 publications

(9 citation statements)

References 32 publications

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“…In addition, there were no signi cant differences in ASGNA protein contents among the three transgenic lines. Previous studies report that expression of GNA protein constituted 0.4% of total soluble protein in transgenic lettuce and 0.3% to 0.4% of the total soluble protein in transgenic potato [37,38]. In this study, the GNA content accounted for 0.41-0.47% of total soluble protein, which is higher than that of.…”

Section: Discussioncontrasting

confidence: 46%

Expression of modified snowdrop lectin (Galanthus nivalis agglutinin) protein confers insect resistance in Arabidopsis and cotton

He¹,

Wu²,

Tian³

et al. 2020

Preprint

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Background: Cotton is a major fiber crop in the world that can be severely infested by pests in agricultural fields. Identifying new insect-resistance genes and increasing expression of known insect-resistance genes are imperative in cultivated cotton. Galanthus nivalis agglutinin, a type of plant lectin that is reportedly toxic towards Homoptera sap-sucking pests when administered in their artificial diet or when fed transgenic plant material expressing the gene. The natural GNA gene is mainly expressed in monocotyledons instead of in dicotyledons, thus modification of the gene is necessary to effectively express the GNA gene in transgenic cotton. Results: We report the artificial synthesis of a modified GNA gene (ASGNA), via codon augmentation, and its insertion into Arabidopsis thaliana and cotton to test its efficacy as an insect-resistance gene against cotton aphids and Plutella xylostella. The level of ASGNA expression in transgenic plants was determined by the enzyme-linked immunosorbent assay (ELISA), and the amount of ASGNA among transgenic plants reached approximately 6.5 μg/g fresh weight. A feeding bioassay showed that survival and reproductive rates of aphids fed transgenic tissues were significantly lower than those fed wild type tissues. Likewise, larvae mortality of P. xylostella fed with transgenic plants showed significantly increased levels. Similar results were recorded of aphids feeding on cotton cotyledons with transient expression of ASGNA. Conclusions: Altogether, results show that ASGNA exhibited high insecticidal activity towards sap-sucking insects and is a promising candidate gene to improve insect resistance in cotton and other dicot plants.

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Section: Discussioncontrasting

confidence: 46%

Expression of modified snowdrop lectin (Galanthus nivalis agglutinin) protein confers insect resistance in Arabidopsis and cotton

He¹,

Wu²,

Tian³

et al. 2020

Preprint

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“…In an another study, the harmful effects of snowdrop lectin (GNA) expressed in transgenic sugarcane on the life cycle of Mexican rice borer Eoreuma loftini (Dyar) and sugarcane borer Diatraea saccharalis (F.) was reported by Sétamou et al (2002) . Expression of Galanthus nivalis lectin (GNA) gene showed resistance to aphids in potato ( Mi et al, 2017 ). Transgenic Arabidopsis expressing recombinant fusion protein (Hv1a/GNA) exhibited resistance to peach potato aphids and grain aphids ( Nakasu et al, 2014 ).…”

Section: Biotechnological Approaches In Insect Pest Managementmentioning

confidence: 99%

Biotechnological Approaches for Host Plant Resistance to Insect Pests

et al. 2022

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Annually, the cost of insect pest control in agriculture crosses billions of dollars around the world. Until recently, broad-spectrum synthetic pesticides were considered as the most effective means of pest control in agriculture. However, over the years, the overreliance on pesticides has caused adverse effects on beneficial insects, human health and the environment, and has led to the development of pesticide resistant insects. There is a critical need for the development of alternative pest management strategies aiming for minimum use of pesticides and conservation of natural enemies for maintaining the ecological balance of the environment. Host plant resistance plays a vital role in integrated pest management but the development of insect-resistant varieties through conventional ways of host plant resistance takes time, and is challenging as it involves many quantitative traits positioned at various loci. Biotechnological approaches such as gene editing, gene transformation, marker-assisted selection etc. in this direction have recently opened up a new era of insect control options. These could contribute towards about exploring a much wider array of novel insecticidal genes that would otherwise be beyond the scope of conventional breeding. Biotechnological interventions can alter the gene expression level and pattern as well as the development of transgenic varieties with insecticidal genes and can improve pest management by providing access to novel molecules. This review will discuss the emerging biotechnological tools available to develop insect-resistant engineered crop genotypes with a better ability to resist the attack of insect pests.

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“…Effects of GNA on M. persicae vary with its expression level in potato plants: at low level GNA reduces colonization of transformed potato, without significant impact on insect performance [119], while highly expressed, GNA can Genetically Modified Potato for Pest Resistance: Thrift or Threat? DOI: http://dx.doi.org/10.5772/intechopen.98748 reduce aphid survival and performance [120]. Besides, GNA can be effective in control of lepidopteran pests -tomato moth (L. oleracea) larvae exhibited about 50% reduction in biomass, prolonged development and 40% increased mortality rate when fed on transformed potato [121].…”

Section: Lectinsmentioning

confidence: 99%

Genetically Modified Potato for Pest Resistance: Thrift or Threat?

Raspor¹,

Cingel²

2021

Solanum Tuberosum - A Promising Crop for Starvation Problem

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Significant limitations in potato production are crop loss due to the damage made by insect pests, and the cost of enormous amount of chemicals, harmful to humans and environment, extensively used in their control. As an alternative, development of genetically modified potato offered possibility for pest management in a more sustainable, environmentally friendly way. Over the past 30 years introduction of pest resistance traits progressed from a single gene to multiple stacked events and from Bt-toxin expression to expression of proteins from non-Bt sources, dsRNA and their combination, while advances in molecular biology have brought “cleaner” gene manipulation technologies. However, together with benefits any new technology also bears its risks, and there are still a range of unanswered questions and concerns about long-term impact of genetically modified crops – that with knowledge and precautionary approaches can be avoided or mitigated. Sustainability of genetically modified crops for pest control largely depends on the willingness to gain and implement such knowledge.

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Expression of the Galanthus nivalis agglutinin (GNA) gene in transgenic potato plants confers resistance to aphids

Cited by 21 publications

References 32 publications

Expression of modified snowdrop lectin (Galanthus nivalis agglutinin) protein confers insect resistance in Arabidopsis and cotton

Expression of modified snowdrop lectin (Galanthus nivalis agglutinin) protein confers insect resistance in Arabidopsis and cotton

Biotechnological Approaches for Host Plant Resistance to Insect Pests

Genetically Modified Potato for Pest Resistance: Thrift or Threat?

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