Expression of the Giant Protein AHNAK (Desmoyokin) in Muscle and Lining Epithelial Cells

Gentil, Benoît J.; Delphin, Christian; Benaud, Christelle; Baudier, Jacques

doi:10.1177/002215540305100309

Cited by 43 publications

(61 citation statements)

References 28 publications

(43 reference statements)

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“…Although the AHNAK protein was originally identified as a nuclear protein, several lines of evidence suggest that the protein is localized in the plasma membrane (3,(31)(32)(33). Gentil et al (31) reported that the main localization of AHNAK is at the plasma membrane in adult muscle cells and the lining of the epithelium .…”

Section: Discussionmentioning

confidence: 99%

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AHNAK-mediated Activation of Phospholipase C-γ1 through Protein Kinase C

Lee

You

et al. 2004

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

“…Gentil et al (31) reported that the main localization of AHNAK is at the plasma membrane in adult muscle cells and the lining of the epithelium . Hashimoto et al (3) reported the PMA-induced translocation of whole AHNAK protein to plasma membrane in FIG.…”

Section: Discussionmentioning

confidence: 99%

AHNAK-mediated Activation of Phospholipase C-γ1 through Protein Kinase C

Lee

You

et al. 2004

Journal of Biological Chemistry

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“…15,52 In both intact and injured spinal cord, AHNAK-IR is found within blood vessels that are also positive for either SMI 71 …”

Section: Intrathecal Chabc Injections Induce Cspg Degradation At the mentioning

confidence: 99%

Astrocytic and Vascular Remodeling in the Injured Adult Rat Spinal Cord after Chondroitinase ABC Treatment

Milbreta

Boxberg

Mailly

et al. 2014

Journal of Neurotrauma

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Upregulation of extracellular chondroitin sulfate proteoglycans (CSPG) is a primary cause for the failure of axons to regenerate after spinal cord injury (SCI), and the beneficial effect of their degradation by chondroitinase ABC (ChABC) is widely documented. Little is known, however, about the effect of ChABC treatment on astrogliosis and revascularization, two important factors influencing axon regrowth. This was investigated in the present study. Immediately after a spinal cord hemisection at thoracic level 8-9, we injected ChABC intrathecally at the sacral level, repeated three times until 10 days post-injury. Our results show an effective cleavage of CSPG glycosaminoglycan chains and stimulation of axonal remodeling within the injury site, accompanied by an extended period of astrocyte remodeling (up to 4 weeks). Interestingly, ChABC treatment favored an orientation of astrocytic processes directed toward the injury, in close association with axons at the lesion entry zone, suggesting a correlation between axon and astrocyte remodeling. Further, during the first weeks post-injury, ChABC treatment affected the morphology of laminin-positive blood vessel basement membranes and vessel-independent laminin deposits: hypertrophied blood vessels with detached or duplicated basement membrane were more numerous than in lesioned untreated animals. In contrast, at later time points, laminin expression increased and became more directly associated with newly formed blood vessels, the size of which tended to be closer to that found in intact tissue. Our data reinforce the idea that ChABC injection in combination with other synergistic treatments is a promising therapeutic strategy for SCI repair.

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“…It is highly expressed in muscle as well as in epithelial and endothelial cell layers with barrier function [4,5,6,7]. First described as desmoyokin in bovine epithelia [8], it was later identified as a tumor-related protein with nuclear localization in cancer cell lines [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…As a component of the L-type Ca 2+ channel complex, Ahnak1 participates in Ca 2+ handling in T cells [24], cardiomyocytes [23], and osteoblasts [25]. Several reports describe a role for Ahnak1 in the cells of the vascular bed [4,7,26,27,28,29]. They describe a distinct Ahnak1 expression in barrier-forming endothelial cells in the blood-brain barrier [7], and indicate a role in lysosome fusion and membrane repair in endothelial cells in vitro [26].…”

Section: Introductionmentioning

confidence: 99%

Protective Function of Ahnak1 in Vascular Healing after Wire Injury

Haase

Rüder²,

Haase³

et al. 2017

J Vasc Res

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Aim: Vascular remodeling following injury substantially accounts for restenosis and adverse clinical outcomes. In this study, we investigated the role of the giant scaffold protein Ahnak1 in vascular healing after endothelial denudation of the murine femoral artery. Methods: The spatiotemporal expression pattern of Ahnak1 and Ahnak2 was examined using specific antibodies and real-time quantitative PCR. Following wire-mediated endothelial injury of Ahnak1-deficient mice and wild-type (WT) littermates, the processes of vascular healing were analyzed. Results: Ahnak1 and Ahnak2 showed a mutually exclusive vascular expression pattern, with Ahnak1 being expressed in the endothelium and Ahnak2 in the medial cells in naïve WT arteries. After injury, a marked increase of Ahnak1- and Ahnak2-positive cells at the lesion site became evident. Both proteins showed a strong upregulation in neointimal cells 14 days after injury. Ahnak1-deficient mice showed delayed vascular healing and dramatically impaired re-endothelialization that resulted in prolonged adverse vascular remodeling, when compared to the WT littermates. Conclusion: The large scaffold and adaptor proteins Ahnak1 and Ahnak2 exhibit differential expression patterns and functions in naïve and injured arteries. Ahnak1 plays a nonredundant protective role in vascular healing.

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Expression of the Giant Protein AHNAK (Desmoyokin) in Muscle and Lining Epithelial Cells

Cited by 43 publications

References 28 publications

AHNAK-mediated Activation of Phospholipase C-γ1 through Protein Kinase C

AHNAK-mediated Activation of Phospholipase C-γ1 through Protein Kinase C

Astrocytic and Vascular Remodeling in the Injured Adult Rat Spinal Cord after Chondroitinase ABC Treatment

Protective Function of Ahnak1 in Vascular Healing after Wire Injury

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