Expression of the Human Tissue-Plasminogen Activator in Hairy Roots of Oriental Melon (Cucumis melo)

Kang, Sol; Ajjappala, Hemavathi; Seo, Hyun-Hui; Sim, Ji Hyun; Yoon, Sang-Hong; Koo, Bon‐Sung; Kim, Yonghwan; Lee, Sukchan; Hahn, Bum‐Soo

doi:10.1007/s11105-011-0303-8

Cited by 11 publications

(4 citation statements)

References 35 publications

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“…Recently, it was demonstrated that the AtMC4 autoproteolytic process is Ca +2 -dependent (Watanabe and Lam 2011). It should be noted that under many stress conditions, e.g., salinity, cold and heat stress, the concentration of cytosolic calcium increases dramatically (Xiong et al 2002; Kang et al 2011). It is also known that high concentrations of calcium ions is toxic for plant organelles; thus, there are some specific channels/pumps that regulate the movement of Ca +2 in and out of cells and organelles (Xiong et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Biochemical and Bioinformatic Characterization of Type II Metacaspase Protein (TaeMCAII) from Wheat

2012

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The biochemical analysis and homology modeling of a tertiary structure of a cereal type II metacaspase protein from wheat (Triticum aestivum), TaeMCAII, are presented. The biochemical characterization of synthetic oligopeptides and protease inhibitors of Escherichia coli-produced and purified recombinant TaeMCAII revealed that this metacaspase protein, similar to other known plant metacaspases, is an arginine/lysine-specific cysteine protease. Thus, a model of a plant type II metacaspase structure based on newly identified putative metacaspase-like template was proposed. Homology modeling of the TaeMCAII active site tertiary structure showed two cysteine residues, Cys140 and 23, in close proximity to the catalytic histidine, most likely participating in proton exchange during the catalytic process. The autoprocessing that leads to activation of TaeMCAII was highly dependent on Cys140. TaeMCAII required high levels of calcium ions for activity, which could indicate its involvement in stress signaling pathways connected to programmed cell death.

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Section: Discussionmentioning

confidence: 99%

Biochemical and Bioinformatic Characterization of Type II Metacaspase Protein (TaeMCAII) from Wheat

2012

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“…Other factors might also be responsible for lower reteplase production in the obtained cell cultures, such as less differentiated cells and plastids (Michoux et al, 2013), instability of the recombinant protein secreted to the medium due to protease release from disrupted cells (Hong et al, 2002), or aggregation occurring when the daughter cells do not separate in the cell division (Santos et al, 2016). Nevertheless, the rtPA yield was significantly higher than the tPA production (up to 0.017%) in a hairy root system (Kang et al, 2011).…”

Section: Determination Of the Protein Content And Activity Assaymentioning

confidence: 99%

Biotechnological production of recombinant tissue plasminogen activator protein (reteplase) from transplastomic tobacco cell cultures

Hidalgo

Abdoli-Nasab

Jalali-Javaran

et al. 2017

Plant Physiology and Biochemistry

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Transplastomic plants are a system of choice for the mass production of biopharmaceuticals due to the polyploidy of the plastid genome and the low risk of pollen-mediated outcrossing because of maternal inheritance. However, as field-grown plants, they can suffer contamination by agrochemicals and fertilizers, as well as fluctuations in yield due to climatic changes and infections. Tissue-type plasminogen activator (tPA), a protein used to treat heart attacks, converts plasminogen into plasmine, which digests fibrin and induces the dissolution of fibrin clots. Recently, we obtained transplastomic tobacco plants carrying the K2S gene encoding truncated human tPA (reteplase) with improved biological activity, and confirmed the presence of the target protein in the transgenic plant leaves. Considering the advantages of plant cell cultures for biopharmaceutical production, we established a cell line derived from the K2S tobacco plants. The active form of reteplase was quantified in cultures grown in light or darkness, with production 3-fold higher in light.

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“…Shake flasks [121] Human tissue plasminogen activator (t-PA) Thrombolytic protein that converts plasminogen into plasmin Oriental Melon (Cucumis melo) Shake flasks [122] [123]…”

Section: Petunia Parodiimentioning

confidence: 99%

Hairy Roots, their Multiple Applications and Recent Patents

Talano

Oller²,

González³

et al. 2012

BIOT

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In the last years, hairy root (HR) cultures are gaining attention in the biotechnology industry. This particular plant cell culture derives from explants infected with Agrobacterium rhizogenes. They constitute a relatively new approach to in vitro plant biotechnology and modern HR cultures are far away from the valuables findings performed by Philip R. White in the 1930's, who obtained indefinite growth of excised root tips. HR cultures are characterized by genetic and biochemical stability and high growth rate without expensive exogenous hormones source. HR cultures have allowed a deep study of plant metabolic pathways and the production of valuable secondary metabolites and enzymes, with therapeutic or industrial application. Furthermore, the potential of HR cultures is increasing continuously since different biotechnological strategies such as genetic engineering, elicitation and metabolic traps are currently being explored for discovery of new metabolites and pathways, as well as for increasing metabolites biosynthesis and/or secretion. Advances in design of proper bioreactors for HR growth are being of great interest, since scale up of metabolite production will allow the integration of this technology to industrial processes. Another application of HR cultures is related to their capabilities to biotransform and to degrade different xenobiotics. In this context, removal assays using this plant model system are useful tools for phytoremediation assays, previous to the application in the field. This review highlights the more recent application of HRs and those new patents which show their multiple utilities.

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Expression of the Human Tissue-Plasminogen Activator in Hairy Roots of Oriental Melon (Cucumis melo)

Cited by 11 publications

References 35 publications

Biochemical and Bioinformatic Characterization of Type II Metacaspase Protein (TaeMCAII) from Wheat

Biochemical and Bioinformatic Characterization of Type II Metacaspase Protein (TaeMCAII) from Wheat

Biotechnological production of recombinant tissue plasminogen activator protein (reteplase) from transplastomic tobacco cell cultures

Hairy Roots, their Multiple Applications and Recent Patents

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