Expression of the <i>MAGE</i> gene family in human head‐and‐neck squamous‐cell carcinomas

Eura, Masao; Ogi, Kojiro; Chikamatsu, Kazuaki; Lee, Kang Dac; Nakano, Koji; Masuyama, Keisuke; Itoh, Kyogo; Ishikawa, Takeru

doi:10.1002/ijc.2910640504

Cited by 62 publications

(61 citation statements)

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“…As the positive rates of MAGE-1 and -3 genes are different among various malignancies (Weynants et al, 1994;Eura et al, 1995aEura et al, , 1995bInoue et al, 1995;Patard et al, 1995;Russo et al, 1995;Shichijo et al, 1995;Toh et al, 1995;Yamada et al, 1995;Corrias et al, 1996;Mori et al, 1996;Muramoto, 1997;Sudo et al, 1997), the expression of the 72-kDa protein might differ between melanomas and HCCs.…”

Section: mentioning

confidence: 99%

Expression of MAGE-1 and -3 genes and gene products in human hepatocellular carcinoma

et al. 1999

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MAGE gene family encodes peptides recognized by autologous cytotoxic T lymphocytes in a major histocompatibility complex (MHC) class-I restricted fashion. In the present study, we have performed reverse-transcription polymerase chain reaction (RT-PCR) for the genes, as well as immunohistochemical analysis and Western blotting of MAGE-1 and -3 proteins in 33 surgically resected hepatocellular carcinomas (HCCs). MAGE-1 and -3 mRNAs were constitutively expressed exclusively in 78 and 42% of HCCs respectively. On immunohistochemistry with monoclonal antibodies, 77B for MAGE-1 and 57B for MAGE-3, MAGE-1 and -3 proteins were recognized in cytoplasm of only six among 33 (18%) and two of 29 HCCs (7%) respectively. The distribution pattern was mostly focal in HCC nodules. By contrast, the Western blot analysis revealed that the MAGE-1 (46 kDa) and -3 proteins (48 kDa) were expressed in 80 and 60% of 15 HCCs examined respectively. The proteins of MAGE-1 and -3 were also expressed exclusively in HCCs regardless of the histological grading and clinical staging. Our results indicate that the detection of the genes by RT-PCR or the proteins by Western blotting is useful for differentiating early HCCs from non-cancerous lesions, and that the peptides derived from MAGE-1 and -3 proteins might be suitable targets for immunotherapy of human HCC. © 1999 Cancer Research Campaign

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Section: mentioning

confidence: 99%

Expression of MAGE-1 and -3 genes and gene products in human hepatocellular carcinoma

et al. 1999

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“…According to previous studies, expression frequencies of the single subtypes were low and heterogeneous [16,23,24]. Thus, analysis of a single MAGE-A seems not to be quite sensitive for the diagnostic evaluation of OSCC.…”

Section: Discussionmentioning

confidence: 89%

“…This study pointed out that MAGE-A1 to A6 should preferably be used for the detection of malignancy in OSCC, because these six genes can be detected concurrently even with a small amount of cells by the MAGE-A1 to A6 assay [15,16,23,24]. It has also previously been determined that the expression frequency of MAGE-A can increase when MAGE-A12 is used as an additional 7th marker [24].…”

Section: Discussionmentioning

confidence: 90%

“…The MAGE-A family consists of 12 subtypes including MAGE-A1 to -A12 [7][8][9][10]. Based on the reverse transcriptase polymerase chain reaction (RT-PCR) typing results for MAGE-A genes, except MAGE-A7 which is not found to be not transcribed, MAGE-A is expressed almost exclusively in various tumor types and testis [9][10][11][12][13][14][15][16][17][18][19]. Expression of single MAGE-A genes has already been extensively examined for the diagnostic application of different malignancies.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, expression frequency was higher when multiple MAGE-A was applied. Previous studies reported that multiple MAGE-A analysis does increase the sensitivity of tumor cell detection in solid tumors including OSCC compared to single MAGE-A analysis [15][16][17][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

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A novel Multiple-Marker Method for the Early Diagnosis of Oral Squamous Cell Carcinoma

et al. 2009

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Abstract. Objective: Melanoma associated antigens-A (MAGE-A) expression is highly specific to cancer cells. Thus, they can be the most suitable targets for the diagnosis of malignancy. The aim of this study was to evaluate the sensitivity of multiple MAGE-A expression analysis for the diagnosis of oral squamous cell carcinoma (OSCC). Methods: Total of 70 OSSC and 20 normal oral mucosal (NOM) samples of otherwise healthy volunteers were examined for the expression of 10 different single antigens out of 12 different MAGE-A subtypes by highly sensitive reverse transcriptase polymerase chain reaction (RT-PCR) methods. The results were correlated to clinicopathological parameters of tumor samples. Results: Expression of MAGE-A was restricted to OSCC. The expression frequency of single antigen was between 10% and 55%. However, expression rate was increased up to 93% by the elevated number of genes examined. A significant correlation was found between the expression of MAGE-A and malignancy (p = 0.0001). In addition, multiple MAGE-A detection has also correlated to the incidence of lymph node metastasis, grading and advanced clinical stages. Conclusions: Analysis of multiple MAGE-A expression is more sensitive than the analysis of a single MAGE-A for the diagnostic evaluation of OSCC. Multiple MAGE-A expression analysis may be a very sensitive method to be used for the diagnosis even in the early stage of OSCC.

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Expression of the MAGE gene family in human hepatocellular carcinoma

Tahara

Mori

Sadanaga

et al. 1999

Cancer

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BACKGROUND The 12 members of the MAGE gene family encode tumor specific antigens that are recognized by autologous cytotoxic T lymphocytes (CTL). The MAGE genes are expressed not only in melanoma but in the other malignant tumors as well. There is, however, little information on their expression in hepatocellular carcinoma (HCC). The authors thus studied the expression of the MAGE gene family in human HCC and discuss the possibility of specific immunotherapy using MAGE peptides. METHODS Tumor tissue samples of HCC and paired nontumor tissues of the liver were obtained from 22 HCC patients. Total RNA was extracted and cDNA was synthesized. Polymerase chain reaction amplification using each MAGE gene specific primer was then performed to detect the expression of each MAGE gene. Immunoblotting and immunohistochemical analysis were performed to confirm the expression of MAGE‐3 gene product in HCC. RESULTS The expression rate of each MAGE gene was as follows: MAGE‐1 and ‐3 were expressed in approximately 68% of the tumors; MAGE‐8 was expressed in 46%; and MAGE‐2, ‐6, ‐10, ‐11, and ‐12 were expressed in approximately 30%. Nineteen (86%) of 22 tumors expressed at least 1 MAGE gene. On the other hand, no expression was detected in the noncarcinomatous liver tissue specimens. Actual expression of the gene product of MAGE‐3 was detected in 50% of tumors. Clinicopathologic data on the MAGE positive and negative cases were compared. Significant differences were observed between MAGE expression status and a few clinicopathologic factors; however, further investigation is required to elucidate these correlations completely. CONCLUSIONS These findings demonstrated that MAGE gene expression is frequent in HCC, thus suggesting that HCC patients may be good candidates for specific immunotherapy using MAGE peptides. Cancer 1999;85:1234–40. © 1999 American Cancer Society.

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Expression of the MAGE gene family in human head‐and‐neck squamous‐cell carcinomas

Cited by 62 publications

References 20 publications

Expression of MAGE-1 and -3 genes and gene products in human hepatocellular carcinoma

Expression of MAGE-1 and -3 genes and gene products in human hepatocellular carcinoma

A novel Multiple-Marker Method for the Early Diagnosis of Oral Squamous Cell Carcinoma

Expression of the MAGE gene family in human hepatocellular carcinoma

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