Expression of the <i>PAX5</i>/BSAP transcription factor in haematological tumour cells and further molecular characterization of the t(9;14)(p13;q32) translocation in B‐cell non‐Hodgkin's lymphoma

Hamada, T; Yonetani, Noboru; Ueda, Chiyoko; Maesako, Yoshitomo; Akasaka, Hiroshi; Ohno, Hitoshi; Kawakami, Keiki; Amakawa, Ryuichi; Okuma, Minoru

doi:10.1046/j.1365-2141.1998.00842.x

Cited by 34 publications

(40 citation statements)

References 40 publications

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“…The sensitivity of this nested PCR method, determined using the t(14;18)-positive lymphoma cell lines TK, 18 FL218, and FL318, 19 appeared to be approximately 1/10 6 cells, and was almost the same as that reported previously in studies of white individuals. [12][13][14][15][16][17] Statistical analysis between the 2 groups was performed using the 2 test.…”

Section: Methodssupporting

confidence: 60%

Low frequency of BCL-2/JH translocation in peripheral blood lymphocytes of healthy Japanese individuals

Yasukawa

Bando

Dölken

et al. 2001

Blood

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The incidence of follicular lymphoma differs significantly between white and Japanese individuals. Translocation between the BCL-2 and immunoglobulin heavy chain genes is detected in 85% to 90% of all follicular lymphomas in whites. Recently, BCL-2/J H translocation was detected in peripheral blood lymphocytes from more than 50% of healthy white individuals. To clarify the reason for the difference in incidence of follicular lymphoma between whites and Japanese, the frequency of BCL-2/J H translocation in peripheral blood lymphocytes of healthy Japanese individuals was compared with that of German individuals. The prevalence of BCL-2/J H translocation in Japanese adults appeared to be significantly lower than that in German adults. The present data suggest that the low frequency of BCL-2/J H translocation in the Japanese general population may be one of the major reasons for the difference in incidence of follicular lymphoma between whites and Japanese. IntroductionThe incidence of follicular lymphoma differs between white and Japanese individuals, accounting for about 40% of all non-Hodgkin lymphomas in whites, compared with less than 10% in Japanese. [1][2][3][4][5] The age-adjusted incidence of follicular lymphoma (per 100 000) is reported to be 3.8 in the United States and 0.5 in Japan. 2 The reason for this difference between the 2 populations is unknown.The translocation t(14;18)(q32;q21) between the BCL-2 protooncogene and the J H immunoglobulin gene region is detected in 85% to 90% and approximately 50% of all follicular lymphomas in whites and Japanese, respectively. [5][6][7][8][9][10] This leads to overexpression of BCL-2, conferring a growth advantage on the neoplastic cells. 11 Recently, it has been reported that BCL-2/J H translocation is frequently detected in peripheral blood lymphocytes of healthy white individuals. [12][13][14][15][16][17] In the multihit theory of tumorigenesis, translocation of the BCL-2 gene is considered to be the first somatic mutation, and additional mutations are needed for development of follicular lymphoma. From this viewpoint, the low incidence of follicular lymphoma in Japanese individuals may be due to the low frequency of BCL-2/J H translocation in the general population or other factors affecting lymphomagenesis. Accordingly, we compared the frequency of BCL-2/J H translocation in peripheral blood lymphocytes of Japanese individuals with that of German individuals. The results showed that the incidence of BCL-2/J H translocation in healthy Japanese individuals is significantly lower than that in German individuals. Study designPeripheral blood mononuclear cells were obtained with informed consent from Japanese and German (white) individuals who had no serious diseases, and the DNA was extracted using standard procedures. The DNA samples from German individuals were sent to Japan and used for experiments. The presence of the BCL-2/J H translocation in the major breakpoint region (MBR) of the BCL-2 gene was examined using a nested polymerase chain reaction (PCR), a...

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Section: Methodssupporting

confidence: 60%

Low frequency of BCL-2/JH translocation in peripheral blood lymphocytes of healthy Japanese individuals

Yasukawa

Bando

Dölken

et al. 2001

Blood

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“…BSAP/Pax5 is a transcription factor also necessary for early B cell development (68,69). It was observed that the BSAP/Pax5 gene was strongly expressed in B cell lymphomas through its translocation into Ig heavy chain locus (70,71). In addition, antisense oligonucleotide-mediated suppression of BSAP activity caused growth inhibition of B cells, but not of T lymphoma or plasma cell lines (72).…”

Section: Notch1 Inhibition Of B Cell Proliferation 36529mentioning

confidence: 99%

Cell Cycle Arrest and Apoptosis Induced by Notch1 in B Cells

Morimura¹,

Goitsuka²,

Zhang³

et al. 2000

Journal of Biological Chemistry

131

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“…By definition, the World Health Organization criteria are stringent for the diagnosis of LPL, excluding plasmacytoid variants of other lymphomas. 9 The heterogeneity of lymphomas associated with t(9;14) is also illustrated by previous reports of sequenced PAX5 breakpoints as shown in Figure 1: a plasmacytoid small lymphocytic lymphoma (1052), 6 a splenic marginal zone lymphoma (MZL-1), 10 a diffuse large B-cell lymphoma (895), 8,11 ␣-heavy chain disease (MAL), 12,13 and the KIS-1 cell line. 4,5 Given the frequency of low-grade B-cell lymphomas other than LPL associated with t(9;14), we chose to investigate the incidence of the PAX5 gene rearrangement in a series of low-grade B-cell lymphomas with an emphasis on plasmacytic differentiation.…”

mentioning

confidence: 84%

“…6,7 Encoding B-cell-specific activator protein, a B-cell transcription factor, the PAX5 gene has been thought to contribute to the development of lymphoma when brought into close proximity of the IgH. 8 The incidence of this translocation has been cited in at least one textbook as involving ϳ50% of LPL, 9 when in fact only a small number of cases have been previously studied.…”

mentioning

confidence: 99%

Low-Grade B-Cell Lymphomas With Plasmacytic Differentiation Lack PAX5 Gene Rearrangements

George

Wrede

Bangs

et al. 2005

The Journal of Molecular Diagnostics

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The chromosomal translocation t(9;14)(p13;q32) has been reported in association with lymphoplasmacytic lymphoma (LPL). Although this translocation involving the paired homeobox-5 (PAX5) gene at chromosome band 9p13 and the immunoglobulin heavy chain (IgH) gene at 14q32 has been described in ϳ50% of LPL cases, the actual number of cases studied is quite small. Many of the initial cases associated with t(9;14)(p13;q32) were actually low-grade B-cell lymphomas with plasmacytic differentiation other than LPL. Thus, we analyzed a series of low-grade B-cell lymphomas for PAX5 gene rearrangements. We searched records from the Department of Pathology, Stanford University Medical Center for low-grade Bcell lymphomas, with an emphasis on plasmacytic differentiation, that had available paraffin blocks or frozen tissue. We identified 37 cases, including 13 LPL, 18 marginal zone lymphomas (nodal, extranodal, splenic, and ␣-heavy chain disease), and 6 small lymphocytic lymphomas. A novel dual-color breakapart bacterial artificial chromosome probe was designed to flank the PAX5 gene, spanning previously described PAX5 breakpoints, and samples were analyzed by interphase fluorescence in situ hybridization. All cases failed to demonstrate a PAX5 translocation, indicating that t(9;14)(p13;q32) and other PAX5 translocations are uncommon events in lowgrade B-cell lymphomas with plasmacytic differentiation. This study also confirms recent reports that found an absence of PAX5 rearrangements in LPL, suggesting the reassessment of PAX5 rearrangements in LPL. Recent studies 1-3 have called into question the association of t(9;14)(p13;q32) and lymphoplasmacytic lymphoma (LPL), both medullary (ie, Waldenströ m's macroglobulinemia) and extramedullary. Initially described in a CD30-positive diffuse large B-cell lymphoma cell line, 4 this translocation involves the paired homeobox-5 (PAX5) gene at 9p13 and the immunoglobulin heavy chain (IgH) gene at 14q32.5 After its initial characterization, further studies found the t(9;14)(p13;q32) associated with LPL.

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Expression of the PAX5/BSAP transcription factor in haematological tumour cells and further molecular characterization of the t(9;14)(p13;q32) translocation in B‐cell non‐Hodgkin's lymphoma

Cited by 34 publications

References 40 publications

Low frequency of BCL-2/JH translocation in peripheral blood lymphocytes of healthy Japanese individuals

Low frequency of BCL-2/JH translocation in peripheral blood lymphocytes of healthy Japanese individuals

Cell Cycle Arrest and Apoptosis Induced by Notch1 in B Cells

Low-Grade B-Cell Lymphomas With Plasmacytic Differentiation Lack PAX5 Gene Rearrangements

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