Expression of the integrin genes in the developing cochlea of rats

Toyama, Katsuhiro; Ozeki, Masashi; Hamajima, Yuki; Lin, Jizhen

doi:10.1016/j.heares.2004.04.019

Cited by 18 publications

(12 citation statements)

References 29 publications

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“…mice. A recently reported study of integrin expression during rat cochlear development (Toyama et al, 2005) is an example of this type of array-based application.…”

Section: Dna Array Analysismentioning

confidence: 99%

Gene expression profiling analysis of the inner ear

et al. 2007

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“…mice. A recently reported study of integrin expression during rat cochlear development (Toyama et al, 2005) is an example of this type of array-based application.…”

Section: Dna Array Analysismentioning

confidence: 99%

Gene expression profiling analysis of the inner ear

et al. 2007

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“…Thus far, only a handful of adhesion-related molecules have been identified and their distributions in the cochlea documented (Davies and Holley, 2002; Simonneau et al, 2003; Suzuki and Harris, 1995; Toyama et al, 2005; Tsuprun and Santi, 1999; Whitlon and Rutishauser, 1990). These molecules belong to multiple adhesion gene families, including the immunoglobulin superfamily and the integrin and cadherin families.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional changes in adhesion-related genes are site-specific during noise-induced cochlear pathogenesis

Cai¹,

Patel²,

Coling³

et al. 2012

Neurobiology of Disease

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Cell-cell junctions and junctions between cells and extracellular matrix are essential for maintenance of the structural and functional integrity of the cochlea, and are also a major target of acoustic trauma. While morphological assessments have revealed adhesion dysfunction in noise-traumatized cochleae, the molecular mechanisms responsible for adhesion disruption are not clear. Here, we screened the transcriptional expression of 49 adhesion-related genes in normal rat cochleae and measured the expression changes in the early phases of cochlear pathogenesis after acoustic trauma. We found that genes from four adhesion families, including the immunoglobulin superfamily and the integrin, cadherin, and selectin families, are expressed in the normal cochlea. Exposure to an intense noise at 120 dB sound pressure level (SPL) for 2 h caused site-specific changes in expression levels in the apical and the basal sections of the sensory epithelium. Expression changes that occurred in the cochlear sensory epithelium were biphasic, with early upregulation at 2 h post-noise exposure and subsequent downregulation at 1 day post-exposure. Importantly, the altered expression level of seven genes (Sgce, sell, Itga5, Itgal, Selp, Cntn1 and Col5a1) is related to the level of threshold shift of the auditory brainstem response (ABR), an index reflecting functional change in the cochlea. Notably, the genes showing expression changes exhibited diverse constitutive expression levels and belong to multiple adhesion gene families. The finding of expression changes in multiple families of adhesion genes in a temporal fashion (2 h vs. 1 day) and a spatial fashion (the apical and the basal sensory epithelia as well as the lateral wall tissue) suggests that acoustic overstimulation provokes a complex response in adhesion genes, which likely involves multiple adhesion-related signaling pathways.

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“…As with LN, a temporo-spatial expression pattern of FN in the path of the SG dendrites was observed in mouse, rat and gerbil (Woolf et al, 1992;Cosgrove and Rodgers, 1997), suggesting a possible function in dendrite targeting. In addition, Toyama et al (2005) identified various isoforms of the integrin ECM receptor family, previously found to be important in the developing brain, in the perinatal mammalian cochlea. These heterodimeric transmembrane proteins, consisting of one a and one b chain, bind promiscuously to various ECM molecules, promoting adherence and functionally linking them to the actin cytoskeleton and other intracellular effectors.…”

Section: Introductionmentioning

confidence: 99%

Laminin and fibronectin modulate inner ear spiral ganglion neurite outgrowth in an in vitro alternate choice assay

Evans

Euteneuer

Chávez

et al. 2007

Developmental Neurobiology

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Extracellular matrix (ECM) molecules have been shown to function as cues for neurite guidance in various populations of neurons. Here we show that laminin (LN) and fibronectin (FN) presented in stripe micro-patterns can provide guidance cues to neonatal (P5) inner ear spiral ganglion (SG) neurites. The response to both ECM molecules was dose-dependent. In a LN versus poly-L-lysine (PLL) assay, neurites were more often observed on PLL at low coating concentrations (5 and 10 microg/mL), while they were more often on LN at a high concentration (80 microg/mL). In a FN versus PLL assay, neurites were more often on PLL than on FN stripes at high coating concentrations (40 and 80 microg/mL). In a direct competition between LN and FN, neurites were observed on LN significantly more often than on FN at both 10 and 40 microg/mL. The data suggest a preference by SG neurites for LN at high concentrations, as well as avoidance of both LN at low and FN at high concentrations. The results also support a potential model for neurite guidance in the developing inner ear in vivo. LN, in the SG and osseus spiral lamina may promote SG dendrite growth toward the organ of Corti. Within the organ of Corti, lower concentrations of LN may slow neurite growth, with FN beneath each row of hair cells providing a stop or avoidance signal. This could allow growth cone filopodia increased time to sample their cellular targets, or direct the fibers upward toward the hair cells.

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Expression of the integrin genes in the developing cochlea of rats

Cited by 18 publications

References 29 publications

Gene expression profiling analysis of the inner ear

Gene expression profiling analysis of the inner ear

Transcriptional changes in adhesion-related genes are site-specific during noise-induced cochlear pathogenesis

Laminin and fibronectin modulate inner ear spiral ganglion neurite outgrowth in an in vitro alternate choice assay

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