Expression of the lncRNA ZFAS1 in cervical cancer and its correlation with prognosis and chemosensitivity

Feng, Lanlan; Shen, Fu‐Ming; Zhou, Jinhua; Chen, Youguo

doi:10.1016/j.gene.2019.01.025

Cited by 47 publications

(33 citation statements)

References 35 publications

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“…Extensive research has confirmed aberrant expression of lncRNAs in cervical cancer. 21,47,48 The dysregulation of lncRNAs has been demonstrated to be crucial for cervical carcinogenesis and cervical cancer progression through the control of a wide range of aggressive characteristics of cancer cells. [49][50][51] Accordingly, lncRNAs may be promising RNAs for the identification of novel chemotherapeutic medications.…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression

Dai

Wei

Zhang

et al. 2020

CMAR

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Purpose: A long noncoding RNA called ZFPM2 antisense RNA 1 (ZFPM2-AS1) has been verified as a key modulator in multiple human cancer types. Nonetheless, the expression and functions of ZFPM2-AS1 in cervical cancer remain poorly understood. Therefore, our purpose was to characterize the expression pattern, clinical value, and detailed roles of ZFPM2-AS1 in cervical cancer. Methods: Reverse-transcription quantitative PCR was carried out to measure ZFPM2-AS1 expression in cervical cancer. A Cell Counting Kit-8 assay, flow cytometry, Transwell migration and invasion assays, and a tumor xenograft experiment were conducted to determine the influence of ZFPM2-AS1 on cervical cancer cell proliferation, apoptosis, migration, and invasion in vitro and on tumor growth in vivo, respectively. Results: ZFPM2-AS1 was found to be aberrantly upregulated in cervical cancer, and its upregulation was associated with unfavorable values of clinical parameters. A ZFPM2-AS1 knockdown significantly reduced cervical cancer cell proliferation, migration, and invasion and increased apoptosis in vitro. The ZFPM2-AS1 knockdown decelerated tumor growth of cervical cancer cells in vivo. Molecular investigation indicated that ZFPM2-AS1 acts as a molecular sponge of microRNA-511-3p (miR-511-3p) in cervical cancer cells. Fibroblast growth factor receptor 2 (FGFR2) mRNA was validated as a direct target of miR-511-3p in cervical cancer, and its expression was positively modulated by ZFPM2-AS1. The effects of the ZFPM2-AS1 knockdown on malignant characteristics of cervical cancer cells were greatly attenuated by miR-511-3p inhibition. Conclusion: ZFPM2-AS1 promotes cervical cancer progression through upregulation of miR-511-3p-FGFR2 axis output, thereby pointing to possible diagnostics and therapeutics based on the ZFPM2-AS1-miR-511-3p-FGFR2 pathway.

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Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression

Dai

Wei

Zhang

et al. 2020

CMAR

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“…5,6 LncRNAs were also involved in the tumorigenesis of OSCC, mainly by acting as miRNA sponges to down-regulate miRNAs on mRNAs. 7,8 Zinc finger antisense 1 (ZFAS1) is a protooncogene that is aberrantly expressed in diverse cancers, including ovarian cancer, 9 cervical cancer, 10 gastric cancer. 11 By interacting with diverse molecules, ZFAS1 regulates different processes including cell cycle, metastasis, invasion, proliferation and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

ZFAS1 Promotes Cisplatin Resistance via Suppressing miR-421 Expression in Oral Squamous Cell Carcinoma

Wang¹,

Hao²,

Wang³

et al. 2020

CMAR

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Oral squamous cell carcinoma (OSCC), with high incidence and mortality, represents one of the main reasons for head and neck malignant tumors. We want to investigate the effect of ZFAS1 on DDP resistance in oral squamous cell carcinoma. Methods: The proliferation and migration of cells was detected by CCK-8 and Transwell assay. The apoptosis was measured by flow cytometry and Western blot. The interaction of ZFAS1, miR-421, and MEIS2 was verified by luciferase reporter assay. The role of ZFAS1 in DDP resistance in vivo was tested by the nude mice model. The expression of ZFAS1 in exosomes from cisplatin-resistant patients was also determined. Results: ZFAS1 overexpression improved OSCC cell growth and inhibited OSCC cell susceptibility to DDP. In addition, the silencing of ZFAS1 promoted DDP-induced apoptosis. ZFAS1 directly bound to miR-421, which was verified by luciferase reporter assay. Inhibition of miR-421 reversed the effect of si-ZFAS1, which promoted the cell viability and decreased the sensitivity of DDP in DDP-resistant cells. The in vivo experiment showed the role of ZFAS1 in increasing the DDP resistance in OSCC tumor. Importantly, this study also showed upregulated ZFAS1 in serum exosomes derived from cisplatin-resistant patients. Conclusion: ZFAS1 promotes chemoresistance of oral squamous cell carcinoma to cisplatin and might become a latent therapeutic target for treating OSCC.

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“…26 These contradictory functions may be attributed to different characteristic and type of human cancers. Considering the oncogenic role in AML and chemoresistance-related effect of ZFAS1, [15][16][17] the present study started from the hypothesis that ZFAS1 might enhance the development of AML chemoresistance. To conrm this, we rstly determined ZFAS1 expression in the BM samples of patients with pediatric AML and AML cells in response to ADR treatment.…”

Section: Discussionmentioning

confidence: 99%

“…15,16 A recent document manifested that ZFAS1 promoted cervical cancer cell chemoresistance to cisplatin. 17 Nevertheless, the role and underlying mechanism of ZFAS1 on AML chemoresistance remain undened.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA ZFAS1 enhances adriamycin resistance in pediatric acute myeloid leukemia through the miR-195/Myb axis

Wang

2019

RSC Adv.

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Expression of the lncRNA ZFAS1 in cervical cancer and its correlation with prognosis and chemosensitivity

Cited by 47 publications

References 35 publications

<p>Long Noncoding RNA <em>ZFPM2-AS1</em> Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression</p>

<p>Long Noncoding RNA <em>ZFPM2-AS1</em> Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression</p>

<p>ZFAS1 Promotes Cisplatin Resistance via Suppressing miR-421 Expression in Oral Squamous Cell Carcinoma</p>

Long noncoding RNA ZFAS1 enhances adriamycin resistance in pediatric acute myeloid leukemia through the miR-195/Myb axis

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