Expression of the maize MYB transcription factor ZmMYB3R enhances drought and salt stress tolerance in transgenic plants

Wu, Jiandong; Jiang, Yingli; Liang, Yani; Chen, Long; Chen, Weijun; Cheng, Beijiu

doi:10.1016/j.plaphy.2019.02.010

Cited by 151 publications

(73 citation statements)

References 39 publications

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“…The ribosomal proteins identified in this study will make viable candidates to study their involvement in abiotic stress tolerance. The role of MYB and MYB-related TFs in salinity stress tolerance has been studied in numerous plants including grasses like alfalfa 31 and food crops like maize 32 . LYR motif containing proteins along with NADH-ubiquinone oxidoreductase are components of the mitochondrial complex I, the first complex in the respiratory chain.…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome assembly and analysis of Phragmites karka, an invasive halophyte, to study the mechanism of salinity stress tolerance

Nayak

Pradhan

Parida

2020

Sci Rep

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With the rapidly deteriorating environmental conditions, the development of stress tolerant plants has become a priority for sustaining agricultural productivity. therefore, studying the process of stress tolerance in naturally tolerant species hold significant promise. Phragmites karka is an invasive plant species found abundantly in tropical and sub tropical regions, fresh water regions and brackish marshy areas, such as river banks and lake shores. the plant possesses the ability to adapt and survive under conditions of high salinity. We subjected P. karka seedlings to salt stress and carried out whole transcriptome profiling of leaf and root tissues. Assessing the global transcriptome changes under salt stress resulted in the identification of several genes that are differentially regulated under stress conditions in root and leaf tissue. A total of 161,403 unigenes were assembled and used as a reference for digital gene expression analysis. A number of key metabolic pathways were found to be over-represented. Digital gene expression analysis was validated using qRt-pcR. in addition, a number of different transcription factor families including WRKY, MYB, CCCH, NAC etc. were differentially expressed under salinity stress. Our data will facilitate further characterisation of genes involved in salinity stress tolerance in P. karka. the DeGs from our results are potential candidates for understanding and engineering abiotic stress tolerance in plants.

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Section: Discussionmentioning

confidence: 99%

De novo transcriptome assembly and analysis of Phragmites karka, an invasive halophyte, to study the mechanism of salinity stress tolerance

Nayak

Pradhan

Parida

2020

Sci Rep

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“…In recent years, several families of plant TFs including MYC, MYB, bZIP, NAC, WRKY, and AP2 / ERF have been identified and proved that play key roles in drought tolerance [8,9]. In Arabidopsis, it has been shown that the expression of maize ZmMYB3R improves drought and salinity tolerance [10]. The expression of Soybean GmNAC085 transcription factor in Arabidopsis significantly led to improved drought tolerance of transgenic plants by positively regulating growth rate and reducing transpiration and cell membrane damage [11].…”

Section: Introductionmentioning

confidence: 99%

Transcription Factors and microRNA Genes Regulatory Network Construction Under Drought Stress in Sesame (Sesamum indicum L.)

Baghery

Kazemitabar

Dehestani

et al. 2020

Preprint

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Background: Drought is one of the most common environmental stresses affecting crops yield and quality. Sesame is an important oilseed crop that most likely faces drought during its growth due to growing in semi-arid and arid areas. Plants responses to drought controlled by regulatory mechanisms. Despite this importance, there is little information about Sesame regulatory mechanisms against drought stress. Results: 458 drought-related genes were identified using comprehensive RNA-seq data analysis of two susceptible and tolerant sesame genotypes under drought stress. These drought-responsive genes were included secondary metabolites biosynthesis-related Like F3H, sucrose biosynthesis-related like SUS2, transporters like SUC2, and protectives like LEA and HSP families. Interactions between identified genes and regulators including TFs and miRNAs were predicted using bioinformatics tools and related regulatory gene networks were constructed. Key regulators and relations of Sesame under drought stress were detected by network analysis. TFs belonged to DREB (DREB2D), MYB (MYB63), ZFP (TFIIIA), bZIP (bZIP16), bHLH (PIF1), WRKY (WRKY30) and NAC (NAC29) families were found among key regulators. mRNAs like miR399, miR169, miR156, miR5685, miR529, miR395, miR396, and miR172 also found as key drought regulators. Furthermore, a total of 117 TFs and 133 miRNAs that might be involved in drought stress were identified with this approach. Conclusions: Most of the identified TFs and almost all of the miRNAs are introduced for the first time as potential regulators of drought response in Sesame. These regulators accompany with identified drought-related genes could be valuable candidates for future studies and breeding programs on Sesame under drought stress. Keywords: Sesamum indicum, Drought stress, Regulatory networks, miRNA, Transcription Factors.

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“…al [86], observed that C 2 H 2 and bHLH TF factors were involved in drought stress response in maize leaves. Additionally, Zhang et al [92] observed that several NACs, MYBs, bZIPs, bHLHs and other TFs expression was tightly coupled to plant water potential, indicating their involvement in Medicago truncatula L. drought adaptation responses. Furthermore, 49 TFs from bHLH, bZIP, C2H2, MYB and NAC families were found in maize spatio-temporal drought stress response [37].…”

Section: Regulation Of Drought Stress By Transcription Factorsmentioning

confidence: 99%

Global transcriptome and weighted gene co-expression network analyses of growth-stage-specific and hybrid-cultivar-specific drought stress responses in maize

Liu¹,

Zenda²,

Jin³

et al. 2020

Preprint

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Background Drought is the major abiotic stress threatening maize ( Zea mays L.) production globally. Therefore, deciphering the molecular basis of maize drought tolerance remains pertinent. Results Here, through a comprehensive comparative leaf transcriptome analysis of drought-tolerant hybrid ND476 plants subjected to water-sufficient (control) and water-deficit (drought) treatment conditions at four (V12, VT, R1, and R4) crop growth stages, we report key cultivar-specific and growth-stage-specific molecular mechanisms regulating drought stress responses in maize. Based on the transcriptome analysis, a total of 3451 differentially expressed genes (DEGs) were identified from the four experimental comparisons, with 2403, 650, 397 and 313 DEGS observed at the V12, VT, R1, and R4 stages, respectively. The expression changes in these genes effected corresponding metabolic pathway responses related to drought tolerance in maize. Subsequently, 3451 DEGs were divided into 12 modules by weighted gene co-expression network analysis (WGCNA), comprising 277 hub genes covering drought-responsive genes involved in water-deficit stress and developmental signaling crosstalk. Interestingly, the co-expressed genes that clustered into similar modules exhibited diverse expression tendencies and got annotated to different GO terms at different stages. MapMan analysis revealed that DEGs related to stress signal transduction, detoxification, transcription factor regulation, hormone signaling and secondary metabolites biosynthesis were universal across the four growth stages. However, the DEGs associated with photosynthesis and amino acid metabolism; protein degradation; transport; and RNA transcriptional regulation were uniquely enriched at the V12, VT, R2, and R4 stages, respectively. Conclusions Our results affirmed that maize drought stress adaptation is a whole-plant response as well as a stage-specific response process. We hope that our findings will aid in clarifying the fundamental cultivar-specific and growth-stage-specific molecular mechanisms regulating drought stress responses in maize. In addition, the genes and metabolic pathways identified here can be valuable genetic resources or selection targets for developing new drought resistant maize cultivars.

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Expression of the maize MYB transcription factor ZmMYB3R enhances drought and salt stress tolerance in transgenic plants

Cited by 151 publications

References 39 publications

De novo transcriptome assembly and analysis of Phragmites karka, an invasive halophyte, to study the mechanism of salinity stress tolerance

De novo transcriptome assembly and analysis of Phragmites karka, an invasive halophyte, to study the mechanism of salinity stress tolerance

Transcription Factors and microRNA Genes Regulatory Network Construction Under Drought Stress in Sesame (Sesamum indicum L.)

Global transcriptome and weighted gene co-expression network analyses of growth-stage-specific and hybrid-cultivar-specific drought stress responses in maize

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