Expression of the monocarboxylate transporter 1 (MCT1) in cells of the porcine intestine

Abstract

Uptake of energy into cells and its allocation to individual cellular compartments by transporters are essential for tissue homeostasis. The present study gives an analysis of MCT1 expression and its cellular occurrence in the porcine intestine. Tissue portions from duodenum, jejunum, ileum, colon ascendens, colon transversum and colon descendens were collected and prepared for immunohistochemistry, Western blot and real time RT-PCR. A 169bp porcine MCT1 cDNA fragment was amplified and published. MCT1 mRNA expression in the large intestine was 20 fold higher compared to the small intestine. Western blot detected a single protein band of 41kDa at a much higher amount of MCT1 protein in the large intestine vs. the small intestine. MCT1 protein was detected in mitochondrial fractions of the large but not the small intestine. Immunohistochemistry in the small intestine showed that immune cells in the lamina propria and in the lymphoid follicles primarily expressed MCT1 while in the colon epithelial cells were the main source of MCT1. In summary, cellular expression of MCT1 differs between epithelial cells in the colon and small intestine. A possible role of MCT1 for uptake of butyrate into immune cells and the overall role of MCT1 for intestinal immune cell function remains elusive.

ORIGINAL ARTICLE: Inulin-coated butyrate increases ileal MCT1 expression and affects mucosal morphology in the porcine ileum by reduced apoptosis

“…Following steps including dehydration and embedding in paraffin are described elsewhere (Claus et al., 2003). Tissue pieces were preserved in RNA stabilizer solution as described by Welter and Claus (2008) for mRNA analysis. Chyme samples from each intestinal section were collected and frozen at −20 °C for inulin determination.…”

“…Expression of MCT1‐mRNA was measured as described earlier (Welter and Claus, 2008) using ubiquitin (UQ) as a housekeeping gene. Threshold cycle (Ct) values were determined by setting the fluorescence threshold at 0.125 and calculating ΔCt by Ct MCT1 ‐ Ct UQ .…”

Probiotic metabolites as epigenetic targets in the prevention of colon cancer

“…In particular, the Lactobacillus spp. are ubiquitously present in fermented foods, vegetation, the oral cavity, the gastrointestinal (GI) ecosystem, and the urogenital tract and are found to confer multiple health‐promoting attributes …”

Expression of Metabolic Coupling and Adhesion Proteins in the Porcine Optic-Nerve Head: Relevance to a Flow Model of Glaucoma

“…Welter and Claus (2008) point to the band of 41 kDa for MCT1 of porcine origin, while Santa Cruz Biotechnology situates MCT1 of human origin between 43 and 50 Kda, and Abcam Laboratories around 52 Kda. For MCT2 of human origin, Santa Cruz biotechnologies predicts a band in the 43 KDa mark, and Abcam in the band of 52 KDa.…”