Expression of the mosquitocidal-protein genes ofBacillus thuringiensis subsp.israelensis and the herbicide-resistance genebar inSynechocystis PCC6803

Chungjatupornchai, Wipa

doi:10.1007/bf02092092

Cited by 52 publications

(30 citation statements)

References 24 publications

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“…Effective expression of the hSOD gene was driven by cyanobacterial promoter sequences from the rbcL-S operon derived from A. nidulans 6301 and was increased by irradiation with light. (8,12,33) or promoters derived from the chloroplast genomes of higher plants (34). Furthermore, investigators have tried to express the foreign genes by using bacterial promoters that are controlled by repressor proteins expressed from the same plasmid (11,15,16).…”

Section: Resultsmentioning

confidence: 99%

High-level expression of human superoxide dismutase in the cyanobacterium Anacystis nidulans 6301.

Takeshima¹,

Takatsugu²,

Sugiura³

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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A chemically synthesized gene encoding human CuZn superoxide disute (hSOD) was loned into the shuttle vector pBAX18R and expressed in Anacysts ins 6301 (Synechococcus sp. strain PCC 6301) under the control of a ribulose-1,5-bisphosphate carboxylase/oxygenase gene (rbc) promoter derived from A. nidulans 6301. The sequences immediately upstream from the hSOD coding region and the distances between the ribosomal binding site and ATG initiation codon strongly affected the expression of the hSOD gene in A. nidulans cells. Optimal expression of hSOD was obtained with the expression vector pBAXSOD8-I, which contained a GGAGAG sequence. In defined conditions, irradiation with light increased hSOD enzyme activity in the transformants >18-fold and the level of the hSOD protein reached a value of about 3% of the total soluble protein. The trnsformants that expressed hSOD acquired the ability to extenuate photooxidative damage induced by methyl viologen.

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Section: Resultsmentioning

confidence: 99%

High-level expression of human superoxide dismutase in the cyanobacterium Anacystis nidulans 6301.

Takeshima¹,

Takatsugu²,

Sugiura³

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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“…Attempts have also been made to express Bt toxins used to control the mosquito vectors of diseases, in aquatic bacteria that inhabit the larval feeding zone (Federici et al 2003). Various bacteria have been manipulated in this way including species of cyanobacteria belonging to the genus Synechococcus (Chungjatupornchai 1990;Soltes-Rak et al 1993), Caulobacter crescentus (Thanabalu et al 1992), Anabaena sp. (Khasdan et al 2003) and Asticcacaulis excentricus (Armengol et al 2005) but, to date, none of these recombinant organisms has been commercially developed.…”

Section: Expression Of Bt Cry Genes In Heterologous Microbial Hostsmentioning

confidence: 98%

Genetic Improvement of Bt Strains and Development of Novel Biopesticides

Sanchis¹

2012

Bacillus Thuringiensis Biotechnology

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This review describes how recombinant DNA technology has been used to improve Bacillus thuringiensis (Bt) products and overcome a number of the problems associated with Bt-based insect control measures. It will discuss how the knowledge of the genetics of Bt and of its insecticidal toxin genes, the understanding of their regulation and the development of cloning vectors has made possible the continuing improvement of first generation products. Several examples describing how biotechnology has been used to increase the production of insecticidal proteins in Bt, their persistence in the field by protecting them against UV degradation or to construct non-viable genetically modified strains, will be presented.

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“…A major breakthrough was the heterologous expression of a functional glucose transporter in the obligate photoautotrophic diatom Phaeodactylum, which enabled the alga to grow on glucose in the dark (30). Another example of successful genetic engineering of a microalga is the expression of mosquito larvacides in cyanobacteria (6,9).…”

Section: Microalgae Genetic Engineeringmentioning

confidence: 99%

Genetic Engineering of Microalgae for Enhanced Biodiesel Production Suitable Fuel Replacement of Fossil Fuel as a Novel Energy Source

Nazari¹,

Raheb

2015

AJLS

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Due to negative environmental influence and limited availability, petroleum derived fuels need to be replaced by renewable biofuels. Biodiesel has attracted intensive attention as an important biofuel. Microalgae provide various potential advantages for biodiesel production when compared with 'traditional' crops. Specifically, large scale micro algal culture need not compete for arable land, while in theory their productivity is greater. In consequence, there has been resurgence in interest and a proliferation of algae fuel projects. However, while on a theoretical basis, microalgae may produce between 10 and 100 fold more oil per acre, such capacities have not been validated on a commercial scale. There are a series of consecutive processes for biodiesel production with microalgae as feedstock, including selection of adequate micro algal strains, mass culture, cell harvesting, oil extraction and trans esterification. To reduce the overall production cost, technology development and process optimization are necessary. Genetic engineering also plays an important role in manipulating lipid biosynthesis in microalgae. Many approaches, such as sequestering carbon dioxide from industrial plants for the carbon source, using wastewater for the nutrient supply, and maximizing the values of byproducts, have shown a potential for cost reduction. This review provides a brief overview of genetic engineering of microalgae for enhanced biodiesel production.

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Expression of the mosquitocidal-protein genes ofBacillus thuringiensis subsp.israelensis and the herbicide-resistance genebar inSynechocystis PCC6803

Cited by 52 publications

References 24 publications

High-level expression of human superoxide dismutase in the cyanobacterium Anacystis nidulans 6301.

High-level expression of human superoxide dismutase in the cyanobacterium Anacystis nidulans 6301.

Genetic Improvement of Bt Strains and Development of Novel Biopesticides

Genetic Engineering of Microalgae for Enhanced Biodiesel Production Suitable Fuel Replacement of Fossil Fuel as a Novel Energy Source

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