Expression of the novel Golgi protein GoPro49 is developmentally regulated during mesenchymal differentiation

Abstract

The Golgi complex is the major cell organelle responsible for protein glycosylation and secretion. In this article, we show that GoPro49 is a new gene expressed specifically in mesenchymal and cartilaginous tissues during development. The corresponding human homologue was identified in our previous Golgi proteomics study and was shown to localize to the Golgi complex as an EGFP-fusion protein. Furthermore, we show using in situ hybridization that GoPro49 expression pattern is both restricted and developmentally regulated. It is specific in vertebrae, ribs, and limbs, and in the craniofacial area in nasal septum and dental follicle. In the trunk, GoPro49 expression decreases before final chondrocyte differentiation, while in the craniofacial area expression is still observed in postnatal tissues. This is the first time a Golgi membrane protein is shown to be expressed in a developmentally regulated manner during mesenchymal and cartilage development in mammals. Developmental Dynamics 237:2243-2255, 2008.

DIA1R Is an X-Linked Gene Related to Deleted In Autism-1

“…Human DIA1 has a known orthologue in both the mouse ( Mus musculus ) and rat ( Rattus norvegicus ) genomes, where it is alternatively called 1190002N15Rik , Ab2-095 or GoPro49 [62]. A search of the HomoloGene database [63], revealed further DIA1 orthologues in the chimpanzee ( Pan troglodytes ), dog (C anis familiaris ), cow ( Bos taurus ), mouse ( Mus musculus ), chicken ( Gallus gallus ), and zebrafish ( Danio rerio ) genomes (data not shown; [64]). Unexpectedly, a Basic Local Alignment Search Tool (BLAST) search [65] of the non-redundant (NCBI) database [63], [66], using the sequence of the human DIA1 gene product, revealed a significantly similar human gene product, LOC79742.…”

Extracellular and Intracellular Signaling for Neuronal Polarity

“…The most strongly upregulated gene is deleted in autism 1 (DIA1, GoPro49). DIA1 is localized to the Golgi complex and is primarily expressed in mesenchymal and cartilaginous tissues but is also mildly expressed in the cortical hem of the cerebral cortex (332). Although this gene has been implicated in autism spectrum disorder (ASD), its function remains unclear (15,226).…”

Novel Golgi Protein, GoPro49, Is a Specific Dental Follicle Marker

“…3C), but not with GM130 (Fig. 3F), was detected as also previously in the chondrosarcoma cell line (Takatalo et al, 2008). The staining intensity of GoPro49 was highly depen dent on the cell passage and decreased rapidly, with highest intensity in primary culture.…”

“…GoPro49 expression has been reported in embryonic mouse molars (Takatalo et al, 2008). We examined the expression during the development of incisors, showing strong and even expression restricted to the dental follicle surrounding the dental epithelium and dental papilla mesenchyme (Fig.…”

“…We have recently reported the expression of GoPro49 in a selection of embryonic mesenchymal tissues, including the cartilage of vertebrae and limbs (Takatalo et al, 2008). In cartilage, the gene was found mostly in columnar chondrocytes, which might indicate a function before terminal differentiation.…”