Expression of the Novel Wheat Gene TM20 Confers Enhanced Cadmium Tolerance to Bakers' Yeast

Kim, Yu-Young; Kim, Doyoung; Shim, Donghwan; Song, Won-Yong; Lee, Joohyun; Schroeder, Julian I.; Kim, Sanguk; Moran, Nava; Lee, Youngsook

doi:10.1074/jbc.m708947200

Cited by 42 publications

(19 citation statements)

References 55 publications

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“…This allowed us to identify three Cd tolerance genes. Two of them were known genes, Phytochelatin syntase 1 (PCS1) (Clemens et al, 1999) and Transmembrane 20 (TM20) (Kim et al, 2008). The third encoded a 432-amino acid polypeptide with homology to heat shock transcription factors.…”

Section: Ta Hsfa4a Plays a Role As CD Tolerance Gene Of Wheatmentioning

confidence: 99%

Orthologs of the Class A4 Heat Shock Transcription Factor HsfA4a Confer Cadmium Tolerance in Wheat and Rice

et al. 2009

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Cadmium (Cd) is a widespread soil pollutant; thus, the underlying molecular controls of plant Cd tolerance are of substantial interest. A screen for wheat (Triticum aestivum) genes that confer Cd tolerance to a Cd hypersensitive yeast strain identified Heat shock transcription factor A4a (HsfA4a). Ta HsfA4a is most similar to the class A4 Hsfs from monocots. The most closely related rice (Oryza sativa) homolog, Os HsfA4a, conferred Cd tolerance in yeast, as did Ta HsfA4a, but the second most closely related rice homolog, Os HsfA4d, did not. Cd tolerance was enhanced in rice plants expressing Ta HsfA4a and decreased in rice plants with knocked-down expression of Os HsfA4a. An analysis of the functional domain using chimeric proteins constructed from Ta HsfA4a and Os HsfA4d revealed that the DNA binding domain (DBD) of HsfA4a is critical for Cd tolerance, and within the DBD, Ala-31 and Leu-42 are important for Cd tolerance. Moreover, Ta HsfA4a-mediated Cd resistance in yeast requires metallothionein (MT). In the roots of wheat and rice, Cd stress caused increases in HsfA4a expression, together the MT genes. Our findings thus suggest that HsfA4a of wheat and rice confers Cd tolerance by upregulating MT gene expression in planta.

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Section: Ta Hsfa4a Plays a Role As CD Tolerance Gene Of Wheatmentioning

confidence: 99%

Orthologs of the Class A4 Heat Shock Transcription Factor HsfA4a Confer Cadmium Tolerance in Wheat and Rice

et al. 2009

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“…Moreover, cadmium triggers accumulation and/or synthesis of reactive oxygen species like superoxide radical, singlet oxygen, hydrogen peroxides, and hydroxyl radicals [11] that may cause cell death by lipid peroxidation, oxidation of proteins [12], damaging DNA [13], and affect the activities of antioxidant enzymes (superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase, catalase, and glutathione reductase) involved in the oxidative defense system [14]. Thus, a balance between the production of activated oxygen species and quenching activity of antioxidant was disturbed [15].…”

Section: Introductionmentioning

confidence: 99%

Response of Maize Seedlings to Cadmium Application after Different Time Intervals

et al. 2013

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Present study was conducted to appraise the inhibitory effects of cadmium applied at different time intervals on various growth and biochemical parameters in two maize lines, Maize-TargetedMutagenesis 1 and 2 (MTM-1 and MTM-2). Twenty-day-old seedlings were exposed to 0, 3, 6, 9, and 12 mg CdCl 2 kg −1 sand. Both maize lines exhibited significant perturbations in important biochemical attributes being employed for screening the crops for cadmium tolerance. The results showed that a higher concentration of cadmium (12 mg CdCl 2 kg −1 ) considerably reduced the plant growth in line MTM-1 on the 5th, 10th, and 15th day after the treatment. In contrast, irrespective of exposure time, the plant biomass and leaf area did not show inhibitory effects of cadmium, specifically at 3 mg CdCl 2 kg −1 in line MTM-2. In addition, MTM-2 was found to be more tolerant than line MTM-1 in terms of lower levels of hydrogen peroxide (H2O2), malondialdehyde (MDA) contents, and relative membrane permeability (RMP). Moreover, H2O2, MDA, RMP, and anthocyanin increased at all levels of cadmium in both lines, but a significant decline was observed in photosynthetic pigments, total free amino acids, and proline contents in all treatments particularly on the 10th and 15th day after treatment.

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“…Proteins of this family are exclusively found in plants, and the gene corresponding to this TP (transcript ID TRINITY_DN46689_c2_g1_i2) was specifically up-regulated in mycorrhizal roots exposed to Cd. Experiments in wheat seedlings demonstrated that expression of TaTM20, a CTEP gene from Triticum aestivum, was induced by Cd treatments and reduced subsequent root Cd accumulation (Kim et al, 2008).…”

Section: Stress Specifically Influences Gene Expression Of Putativmentioning

confidence: 99%

Modulation of Plant and Fungal Gene Expression Upon Cd Exposure and Symbiosis in Ericoid Mycorrhizal Vaccinium myrtillus

et al. 2020

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The success of Ericaceae in stressful habitats enriched in heavy metals has been ascribed to the distinctive abilities of their mycorrhizal fungal partners to withstand heavy metal stress and to enhance metal tolerance in the host plant. Whereas heavy metal tolerance has been extensively investigated in some ericoid mycorrhizal (ERM) fungi, the molecular and cellular mechanisms that extend tolerance to the host plant are currently unknown. Here, we show a reduced Cd content in Cd-exposed mycorrhizal roots of Vaccinium myrtillus colonized by a metal tolerant isolate of the fungus Oidiodendron maius as compared to non-mycorrhizal roots. To better understand this phenotype, we applied Next Generation Sequencing technologies to analyze gene expression in V. myrtillus and O. maius Zn grown under normal and Cd-stressed conditions, in the free living and in the mycorrhizal status. The results clearly showed that Cd had a stronger impact on plant gene expression than symbiosis, whereas fungal gene expression was mainly regulated by symbiosis. The higher abundance of transcripts coding for stress related proteins in non-mycorrhizal roots may be related to the higher Cd content. Regulated plant metal transporters have been identified that may play a role in reducing Cd content in mycorrhizal roots exposed to this metal.

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Expression of the Novel Wheat Gene TM20 Confers Enhanced Cadmium Tolerance to Bakers' Yeast

Cited by 42 publications

References 55 publications

Orthologs of the Class A4 Heat Shock Transcription Factor HsfA4a Confer Cadmium Tolerance in Wheat and Rice

Orthologs of the Class A4 Heat Shock Transcription Factor HsfA4a Confer Cadmium Tolerance in Wheat and Rice

Response of Maize Seedlings to Cadmium Application after Different Time Intervals

Modulation of Plant and Fungal Gene Expression Upon Cd Exposure and Symbiosis in Ericoid Mycorrhizal Vaccinium myrtillus

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