Expression of the potential biomarker heat shock protein 70 and its regulator, the metazoan DnaJ homolog, by temperature stress in the sponge Geodia cydonium

Abstract

Because sponges (Porlfera) are generally the domlnant group of m~~lticellular animals found In the marine benthos they <ire key animals f o~ the testing of biomarkers in thls biotope Prelriously ~t was shoivn that the heat shock protein hsp70 from the manne sponge Geodla cydonlum is a useful biomarkel for following biological responses to selected physical and c h e m~c a l stressois One protein which is cntical for hsp70 function In other organisms 1s the DnaJ-llke protein In this study w e therefore isolated and character~zed a cDYA e n c o d~n g the DnaJ-like proteln from G c)/donlum des~g n a t e d GCDNAJ GCDNAJ 1s 1476 base palls (bp) long 1~1th a 1236 bp open reading frame e n c o d~n g a proteln of 45542 daltons Northern blot dnalys~s revealed a transcript slze of 1 5 kilobase (kb] The deduced amlno acid (aa) secjuence of GCDKAJ contalns motifs charactenstic of most prokaryotic and eukaryotic DnaJ famlly members In response to a 7°C temperature increase, express~on of the transcripts encodlng hsp70 and DnaJ-like protein Increased as much as 18-fold and 3-fold, respectively, within a 8 h penod From these data we conclude that the levels of both hsp70 and DnaJ-11ke proteln are likely to be important to the adaptation to temperature increases in G cydonlum KEY WORDS: G e o d~a cydonium . DnaJ-like protein . Heat shock proteln . Sponge hsp70

Induction of gene expression of the chaperones <t-1>14-3-3 and HSP70 by PCB 118 (2,3',4,4',5-pentachloro<t0>biphenyl) in the marine sponge Geodia cydonium:novel biomarkers for polychlorinated biphenyls

“…We have shown previously that sponges serve as suitable bioindicator organisms for a series of stressors using the biomarker HSP70 (Koziol et al 1996(Koziol et al , 1997a. In the present study, the level of HSP70 was greatly elevated in sponges treated with PCB 118; the mRNA level as well as the protein level increased several-fold.…”

“…In order to investigate those stress proteins ubiquitously present in invertebrates, the cDNA encoding the HSP70 stress protein from the marine sponge Geodia cydonium (Koziol et al 1996) together with its associated protein DnaJ (Koziol et al 1997a) were cloned. Furthermore, in order to determine the steady-state level of HSP70, antibodies against G. cydonium HSP70 were raised (this contribution).…”

Sponge Bcl-2 homologous protein (BHP2-GC) confers distinct stress resistance to human HEK-293 cells

“…44 ), inducible; it is selectively upregulated in response to non-toxic apoptotic stimuli. At higher TBT concentrations (410 mM) or more severe heat shock (148C above ambient temperature) the expressions of genes coding for BHP2_GC, HSP-70 and septin decline 26,45 (and unpublished).…”

Accumulation of cadmium and zinc in the marine sponge Suberites domuncula and its potential consequences on single-strand breaks and on expression of heat-shock protein:a natural field study

“…Interesting is the finding that in C. elegans the expression of the constitutive HSP70 is very low under nonstressed conditions but increases strongly after incubation in the presence of heat and cadmium (Guven & De Pomerai 1995); this finding suggests that more work is required to understand which heat-shock protein of the M, species 70 000 responds to a given stressor (Guven & De Pomerai 1995). In earlier experiments we demonstrated that sponges also react to heat (Koziol et al 1996), different nonphysiological physical conditions (Koziol et al 1996(Koziol et al , 1997, and xenobiotics (Miiller et al 1995) with an increased expression of the gene(s) coding for HSP70. Therefore, we analyzed whether HSP70 is also a suitable biomarker for increased cadmium load of Suberites domuncula in the environment.…”