Expression of the PVYO coat protein (CP) under the control of the PVX CP gene leader sequence: protection under greenhouse and field conditions against PVYO and PVYN infection in three potato cultivars

Hefferon, Kathleen; Khalilian, Houri; AbouHaidar, Mounir G.

doi:10.1007/s001220050412

Cited by 19 publications

(9 citation statements)

References 23 publications

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“…Most reports evaluating field experiments with transgenic potato plants have dealt with the effects of specific agronomic traits such as increased resistance against insects, viruses and herbicides and chemical composition (Nelson et al, 1988;Delannay et al, 1989;De Greef et al, 1989;Lawson et al, 1990: Jongedijk et al, 1992Van der Vlugt & Goldbach, 1993;Kuipers et al, 1994;Allefs et al, 1995;Borovkov et al, 1996;Hefferon et al, 1997). However, few studies have assessed the quantitative performance of independently transformed plants under various environmental conditions (Dale and McPartlan, 1992;Conner et al, 1994).…”

Section: Performance Of Transgenic Plants Of Potatomentioning

confidence: 97%

Performance of transgenic plants of potato (Solanum tuberosumcv. Laila) grownin vitroin greenhouse and in a field trial

Bones

Honne²,

Nielsen

et al. 1997

Acta Agriculturae Scandinavica, Section B - Soil & Plant Sc

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Performance of transgenic plants of potato (Solatium tuberosum cv. Laila) grown inEffects of transformation, regeneration, and integration of marker genes, on the performance of transgenic potato (Solarium tuberosum) plants were analysed by examination of the regenerated clones at different cultivation stages. Twenty out of 55 clones resistant to kanamycin in vitro were further analysed under greenhouse conditions and, finally, the performance of 4 clones was examined in the field. An enzyme-linked immunosorbent assay (ELISA) was developed to quantify the expression of the marker gene encoded ß-galactosidase. In the field trial, a significant correlation was found between the expression of ß-galactosidase and percent of dry matter of the tubers (r= -0.908, P = 0.033). Furthermore, the variation in infection of haulm by Phytophthora infestans was significant among clones (P = 0.001) and accounted for the variation in tuber weight seen among clones (R 2 = 0.95). A good correlation of the relative tuber yields of r = 0.997 (P = 0.00025) between the greenhouse and the field suggests that greenhouse experiments can be designed which enable the prediction of field performance of transgenic clones.

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Section: Performance Of Transgenic Plants Of Potatomentioning

confidence: 97%

Performance of transgenic plants of potato (Solanum tuberosumcv. Laila) grownin vitroin greenhouse and in a field trial

Bones

Honne²,

Nielsen

et al. 1997

Acta Agriculturae Scandinavica, Section B - Soil & Plant Sc

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“…As most reports of Shepody transformation focus on evaluating the gene of interest rather than transformation improvement (see Chang et al, 1993;Hefferon et al, 1997;Zhang et al, 2001), we selected a transformation method (De Block, 1988) and began optimizing the protocol for Shepody. To this end, we chose the explant-PGR combination that gave the highest regeneration frequency and evaluated the transformation efficiency.…”

Section: Introductionmentioning

confidence: 99%

Transformation and Plant Regeneration from Leaf Explants of Solanum tuberosum L. cv. ‘Shepody’

Gustafson

Mallubhotla

MacDonnell³

et al. 2006

Plant Cell Tiss Organ Cult

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As part of a large-scale genomics project focused on understanding and improving the Shepody potato, we have increased the regeneration and transformation rates for this cultivar. Using combinations of auxins and trans-zeatin, leaf and stem explants were evaluated for callus induction and shoot formation. Several plant growth regulator combinations resulted in higher plant regeneration rates over a previous method. Using the best combination of auxin and cytokinin in combination with Agrobacterium-mediated transformation, we regenerated independent putative transformants from 59.5% of the total explants plated. We ran PCR on a sample of the plants to confirm transformation and 47.1% were nptII positive; giving a confirmed transformation rate of 28.0%.

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“…Coat protein (CP) genes have been shown to confer partial or complete resistance as was observed for TSV in tobacco [23], cucumber mosaic virus in cucumber [14], and potato virus Y in potato plants [7].…”

Section: Introductionmentioning

confidence: 99%

Coat protein-mediated transgenic resistance of peanut (Arachis hypogaea L.) to peanut stem necrosis disease through Agrobacterium-mediated genetic transformation

et al. 2013

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The absence of resistance genes against biotic stresses like Tobacco streak virus (TSV) within compatible peanut germplasm necessitates the deployment of genetic engineering strategy to develop transgenic resistance. Transgenic resistance in peanut (Arachis hypogaea L.) to peanut stem necrosis disease caused by TSV was obtained by transferring coat protein (CP) gene of TSV through Agrobacterium-mediated transformation of de-embryonated cotyledons and immature leaves of peanut cultivars Kadiri 6 (K6) and Kadiri 134 (K134). Integration of the transgene in T 1 , T 2 and T 3 generations were confirmed by PCR with gene-specific primers. On the basis of segregation analysis of the PCR amplicons, homozygosity was confirmed in progeny from five transgenic lines. Six transgenic plants from three different single copy transgenic lines homozygous for the transgene were selected for challenge inoculation in T 3 generations. The transgenic lines remained symptomless throughout and showed traces or no systemic accumulation of virus indicating the tolerance/resistance to the TSV infection. CP gene expression was observed in transgenic lines by RT-PCR, real-time PCR and ELISA. The findings provide an effective strategy for developing peanut with resistance to peanut stem necrosis disease.

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Expression of the PVYO coat protein (CP) under the control of the PVX CP gene leader sequence: protection under greenhouse and field conditions against PVYO and PVYN infection in three potato cultivars

Cited by 19 publications

References 23 publications

Performance of transgenic plants of potato (Solanum tuberosumcv. Laila) grownin vitroin greenhouse and in a field trial

Performance of transgenic plants of potato (Solanum tuberosumcv. Laila) grownin vitroin greenhouse and in a field trial

Transformation and Plant Regeneration from Leaf Explants of Solanum tuberosum L. cv. ‘Shepody’

Coat protein-mediated transgenic resistance of peanut (Arachis hypogaea L.) to peanut stem necrosis disease through Agrobacterium-mediated genetic transformation

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