Expression of the renin gene in extra-renal tissues of the rat

Samani, Nilesh J.; Swales, J. D.; Brammar, William J.

doi:10.1042/bj2530907

Cited by 99 publications

(44 citation statements)

References 32 publications

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“…However the presence of renin mRNA in vascular tissue is still a matter of debate. One report indicated the expression of the renin gene in aortic tissue (21), while this was not confirmed in other studies (203,204).…”

Section: Vasculaturementioning

confidence: 80%

“…In addition, the synthesis of these proteins should be regulated independently of the circulating RAS. These criteria are met in several tissues, e.g., brain, adrenal gland, heart, and vessels, where the mRNA for renin, AOGEN, ACE, and ANG II receptors have been shown to be regulated by physiological or pharmacological eff ectors (18)(19)(20)(21)(22)(23)(24)(25)(26)(27) . The relevance of these systems is confirmed by the fact that chronic treatment with CEI failed to correlate with pretreatment plasma renin activity but did correlate with sustained inhibition of ACE-activity in the kidney (28).…”

Section: Local Renin-angiotensin Systemsmentioning

confidence: 99%

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The Role of the Renin-Angiotensin System in Cardiovascular Disease.

et al. 1994

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Recent findings have increased our understanding of the role of the renin-angiotensin system in cardiovascular diseases. This was possible by the progress made in molecular biology and the import of this methodology into cardiovascular research by several groups in the past few years. Studies by these groups accumulated evidence especially for the existence and physiological importance of organ-based renin-angiotensin systems and their involvement in the pathogenesis of hypertension and the accompanying disorders of the heart, the kidney, and the vessels. This Review will focus mainly on the relevance of the local renin-angiotensin systems for cardiovascular diseases and the value of transgenic animals as models to analyse these systems (Hypertens Res 1994; 17: 1-16).

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Section: Vasculaturementioning

confidence: 80%

Section: Local Renin-angiotensin Systemsmentioning

confidence: 99%

The Role of the Renin-Angiotensin System in Cardiovascular Disease.

et al. 1994

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“…Recent work has, however, demonstrated the presence of renin mRNA in the heart and brain of rats and mice (19,20). Using RNase-protection analyses, a triplet sequence mis-match in exon two (14,15) (21), and renin mRNA has recently been detected by a nuclease-protection assay in rat liver (22). Although the physiological role of renin in the liver is unknown, the findings that angiotensin II can interact with high affinity receptors in rat liver chromatin (23) (25).…”

Section: Discussionmentioning

confidence: 99%

Differential extra-renal expression of the mouse renin genes

Miller¹,

Carter²,

Brooks³

et al. 1989

Nucl Acids Res

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We have used RNase-protection analyses to study renin gene expression in one-and two-gene mouse strains. The RNase-protection assay is capable of discriminating between the transcripts from the different renin genes. In a two-gene strain containing Ren-1D and Ren-2, we demonstrate transcriptional activity from Ren-ID in kidney, submandibular gland (SMG), testes, liver, brain and heart. Ren-2 is clearly expressed in kidney, SMG and testes. Similar analyses of one gene strains (containing show expression in kidney, SMG, testes, brain and heart. We cannot detect renin mRNA in the liver of these mice.Ren-1C and Ren-1D thus display quite different tissue-specificities. In order to determine whether the different tissue-specificities of the highly homologous Ren-lC and Ren-1D genes are due to different trans-acting factors in the different mouse strains or to different cis-acting DNA elements inherent to the genes, we introduced a Ren-11D transgene (Ren-l*) into a background strain containing only the Ren-1C gene. The transgene exhibits the same tissue-specificity as the Ren-1D gene of two-gene strains suggesting the presence of different cis-acting DNA elements in Ren-1C and Ren-1D. INTRODUCTIONThe aspartyl protease renin catalyses the initial and rate-limiting step in the conversion of angiotensinogen to the vasoactive hormone angiotensin II and thus plays a key role in the regulation of blood pressure (1). The primary site of renin biosynthesis is the juxtaglomerular apparatus of the kidney.

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“…As renin mRNA level in vasculature was reported 12 to be very low, we used the polymerase chain reaction method to assess renin mRNA levels in kidney and aorta. The precise method and the validity for the measurement of renin mRNA were described in detail elsewhere.…”

Section: Renin Mrna Measurement By Potymerase Chain Reaction Methodsmentioning

confidence: 99%

“…Swales et al 6 ' 7 demonstrated arterial wall uptake of renal renin, and we have reported 8 the importance of vascular renin of renal origin in maintaining hypertension of spontaneously hypertensive rats, suggesting the participation of plasma-borne components in local angiotensin production. On the other hand, renin synthesis in vascular smooth muscle cells 9 and endothelial cells 10 ' 11 in culture and the expression of the messenger RNA (mRNA) for renin 12 and angiotensinogen 1314 in vascular tissues have suggested a potential role of locally produced components in local angiotensin production. Another important issue is the regulation of local angiotensin generation, about which our understanding is limited.…”

mentioning

confidence: 99%

Regulation of vascular angiotensin release.

et al. 1993

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To investigate the regulatory mechanism of the vascular renin-angiotensin system, we perfused isolated rat hind legs with plasma-free buffer and quantified angiotensin peptides in the perfusate. Angiotensin release from hind legs was increased in rats pretreated with losartan (DuP 753) and rats fed a low sodium diet with subsequent furosemide and was decreased in nephrectomized rats and rats given dexamethasone, ethynylestradiol, and triiodothyronine. Using these models, we have attempted to identify which step or component of angiotensin metabolism determines angiotensin release level. Changes caused by these manipulations in plasma renin concentration and basal angiotensin release from hind legs were almost parallel, whereas plasma angiotensinogen concentration and the angiotensin release changed in opposite directions. Infusion of renin in hind legs caused a marked increase in angiotensin release and continued even 1 hour after cessation of renin infusion. Infusion of angiotensinogen did not alter the angiotensin release. Angiotensin clearance and angiotensin I conversion were not affected by either nephrectomy or losartan pretreatment. Aortic renin messenger RNA level was extremely low and not increased by nephrectomy or losartan pretreatment, although kidney renin messenger RNA level was increased by losartan pretreatment These results provide evidence that plasma renin of kidney origin is the major source of vascular functional renin and plays the determining role in the regulation of vascular angiotensin release. Plasma-derived or locally produced angiotensinogen, locally produced renin, converting enzyme, and angiotensin clearance are not considered to be the primary determinant in the regulation of vascular angiotensin release in these acute and subacute experimental models.

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Expression of the renin gene in extra-renal tissues of the rat

Cited by 99 publications

References 32 publications

The Role of the Renin-Angiotensin System in Cardiovascular Disease.

The Role of the Renin-Angiotensin System in Cardiovascular Disease.

Differential extra-renal expression of the mouse renin genes

Regulation of vascular angiotensin release.

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