Expression of the rpl23, rpl2 and rps19 genes in spinach chloroplasts

Thomas, Frank J.; Massenet, O.; Dorne, Anne-Marie; Briat, Jean‐François; Mache, Régis

doi:10.1093/nar/16.6.2461

Cited by 54 publications

(32 citation statements)

References 15 publications

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“…2, lane 3). These are the transcripts of the rpl23 and rpsl9 genes which are clustered on the plastid DNA in an arrangement similar to the S-10 operon of E. coli [34,35].…”

Section: Resultsmentioning

confidence: 99%

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The components of the plastid ribosome are not accumulated synchronously during the early development of spinach plants

Bisanz-Seyer

Seyer

et al. 1989

Plant Mol Biol

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The expression of components of the 70S plastid ribosome has been determined during the first 13 days of spinach plant development. Total cellular RNA and proteins were used to determine the relative steady-state levels of mRNA for ribosomal proteins (r-proteins) by dot blot hybridization and the relative amounts of proteins by immunodetection with specific antibodies. The 16S rRNA as well as mRNAs for 9 out of 11 proteins studied, including those for the 32 kDa polypeptide of photosystem II and the large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase (Rubisco) show a marked increase at the beginning of the germination (day 5). At this time the plastid DNA content increases from 4% to 6% of total DNA content and so the plastome copy number can only in part account for the important increase in mRNA steady-state levels. Interestingly the transcripts of the rpl23 and rps19 genes show a different accumulation pattern, indicating either a differential gene transcription and/or an increased stability of the transcripts. In the western blot analysis a group of r-proteins can be detected in dry seeds or after 24 hours of imbibition while a second group of proteins accumulates after 3 to 5 days of development. The differential accumulation pattern of r-proteins and mRNA for r-proteins indicates that post-transcriptional control plays an important role in plastid r-protein synthesis.

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“…2, lane 3). These are the transcripts of the rpl23 and rpsl9 genes which are clustered on the plastid DNA in an arrangement similar to the S-10 operon of E. coli [34,35].…”

Section: Resultsmentioning

confidence: 99%

“…3B). These are the transcripts of the rp123 and rpsl9 genes, which are clustered on the plastid DNA in a S10-1ike operon [22,34,35]. The whole cluster comprises the genes rp123, rpil2, rpsl9, rp122, rps3, rpll6, rpll4 and rps8 (genes in bold type have been studied in this paper).…”

Section: Changes In the Steady-state Levels Of Mrna For 70s R-proteinmentioning

confidence: 99%

The components of the plastid ribosome are not accumulated synchronously during the early development of spinach plants

Bisanz-Seyer

Seyer

et al. 1989

Plant Mol Biol

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“…For instance, rpl23, which encodes a protein component of the large ribosomal subunit, is present in Gossypium barbadense and many other plant species, while it is a pseudo-gene in Spinacia oleraceae and has been substituted by a nuclear functional gene (Thomas et al, 1988;Bubuneko et al, 1994;Yamaguchi and Subramanian, 2000). The infA gene, which encodes an initiation factor protein, is present as a pseudo-gene in Gossypium barbadense , which is consistent with its presence in Nicotiana tabacum (Shinozaki et al, 1986) and Atropa belladonna (Schmitz-Linneweber et al, 2002).…”

Section: Pseudo-and True Genesmentioning

confidence: 99%

Complete Nucleotide Sequence of the Cotton (Gossypium barbadense L.) Chloroplast Genome with a Comparative Analysis of Sequences among 9 Dicot Plants

2006

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Recently, the complete chloroplast genome sequences of many important crop plants were determined, and this can be considered a major step forward toward exploiting the usefulness of chloroplast genetic engineering technology. Economically, cotton is one of the most important crop plants for many countries. To further our understanding of this important crop, we determined the complete nucleotide sequence of the chloroplast genome from cotton ( Gossypium barbadense L.). The chloroplast genome of cotton is 160,317 base pairs (bp) in length, and is composed of a large single copy (LSC) of 88,841 bp, a small single copy (SSC) of 20,294 bp, and two identical inverted repeat (IR) regions of 25,591 bp each. The genome contains 114 unique genes, of which 17 genes are duplicated in the IRs. In addition, many open reading frames (ORFs) and hypothetical chloroplast reading frames ( ycf s) with unknown functions were deduced. Compared to the chloroplast genomes from 8 other dicot plants, the cotton chloroplast genome showed a high degree of similarity of the overall structure, gene organization, and gene content. Furthermore, the sequences of the genes showed high degrees of identity at the DNA and amino acid levels. The cotton chloroplast genome was somewhat longer than the chloroplast genomes of most of the other dicot plants compared here. However, this elongation of the cotton chloroplast genome was found to be due mainly to expansions of the intergenic regions and introns (noncoding DNA). Moreover, these expansions occurred predominantly in the LSC and SSC regions.

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“…Nuclear and mitochondrial probes were from maize. The following DNA fragments were used as probes: the 750 bp XbaI-PstI intragenic fragment of the psbA gene (26); the 550 bp SaIl-EcoRV fragment containing the rpsl9 gene (27); the 1200 bp EcoRI-PstI intragenic fragment of the rbcL gene (30); the 1200 bp EcoRI-BamHI intragenic fragment of the rrn 16S gene (1); the 1200 bp EcoRI-HindIII intragenic fragment of the atp6 gene (8); the 2200 bp XbaI fragment containing the atp9 gene (9); the intragenic 680 bp HindIII-EcoRI fragment of the cob gene(6); the 1000 bp EcoRI intragenic fragment ofthe rbcS gene. Labeling ofinserts was done by the random hexamer priming method to a specific activity ranging from 1 to 5 x 109 cpm/,ug.…”

Section: Chloroplast Dna Preparationmentioning

confidence: 99%

Expression of Chloroplast and Mitochondrial Genes during Microsporogenesis in Maize

Monéger

Mandaron²,

Niogret³

et al. 1992

Plant Physiol.

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Mitochondrial and plastid gene expression has been examined during maize (Zea mays) microsporogenesis. Accumulation of transcripts was found for three mitochondrial genes studied (cob, atp6, and atp9) at the mid-term of pollen development. In contrast, these mitochondrial transcripts were undetectable in mature pollen. Southern and DNA gel blot experiments showed that the copy number of mitochondrial genes was amplified in microspores at stages preceding the accumulation of these transcripts. Plastid transcripts of the photosynthetic psbA and rbcL genes could not be detected after the two mitoses, whereas precursors of the 16S rRNA are detected at low levels.Cytoplasmic maternal inheritance and CMS2 are two biological traits showing the importance of organelles during microsporogenesis. In the case of maize, genetic and cytological evidence has been given for the lack of paternal inheritance of plastid and mitochondrial DNA (2, 4, and references therein). Different authors have tried to explain the mechanisms implied in maternal inheritance (5) and different hypotheses have been brought forward. One of these hypotheses suggests (7) destruction of the paternal plastid genome during microsporogenesis in barley to account for maternal inheritance. Knowledge about the persistence and integrity of organelle DNA during pollen formation would therefore help to evaluate this hypothesis. Recently, pollen DNA from Medicago sativa or Antirrhinum majus, known to transmit organelle genes biparentally and maternally, respectively, was probed with a plastid-specific DNA fragment (5). It was shown that the presence or absence of plastid DNA correlated with the type of inheritance. It would be interesting to know whether this result might be generalized and valid in other plant species.In the case of CMS, blocks occur in critical steps in the development of the male flower. CMS has been extensively studied in maize and it is now accepted that the CMS phenotype is correlated with recombination events in the mito-

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Expression of the rpl23, rpl2 and rps19 genes in spinach chloroplasts

Cited by 54 publications

References 15 publications

The components of the plastid ribosome are not accumulated synchronously during the early development of spinach plants

The components of the plastid ribosome are not accumulated synchronously during the early development of spinach plants

Complete Nucleotide Sequence of the Cotton (Gossypium barbadense L.) Chloroplast Genome with a Comparative Analysis of Sequences among 9 Dicot Plants

Expression of Chloroplast and Mitochondrial Genes during Microsporogenesis in Maize

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