Expression of the rtp gene of Bacillus subtilis is required for replication fork arrest at the chromosome terminus

Smith, Marc D.; Wake, R.G.

doi:10.1016/0378-1119(89)90479-4

Cited by 9 publications

(2 citation statements)

References 12 publications

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“…This forked DNA, which appears transiently around the time of termination of a cycle of replication, is dependent upon expression of the rtp gene. Thus, in the absence of rtp, band I is not detected (Smith and Wake, 1988;Smith and Wake, 1989).…”

Section: Construction Of Isogenic Dnab37 Mutant Strairis Tacking or Omentioning

confidence: 99%

A checkpoint involving RTP, the replication terminator protein, arrests replication downstream of the origin during the Stringent Response in Bacillus subtilis

Levine¹,

Autret²,

Sj³

1995

Molecular Microbiology

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Regulation of DNA replication in Bacillus subtilis involves a post-initiation mechanism which is subject to control by the Stringent System, an essential regulatory network, mediated by the alarmone, ppGpp. In detailed studies using DNA-DNA hybridization procedures, we have now shown that, following the induction of the Stringent Response, replication is blocked downstream of the origin, on the left, close to the hut marker (-175 kb) and on the right, beyond the soft10 marker (+199 kb). In addition, we provide evidence that inhibition of replication under these conditions requires the replication terminator protein (RTP). In a mutant lacking RTP, a protein normally involved in termination of chromosomal replication through recognition of specific terminator sequences, replication continues past the sites normally blocked by the Stringent Response. These data strengthen the argument that this second level of control of DNA replication occurs at specific sites, the Strigent Terminus (STer) sites, either side of orlC. Such sites are presumably related to the sequence involved in RTP recognition at the terminus, terC. We propose that the binding of RTP must be modulated, perhaps through the action of ppGpp, to recognize post-initiation control sequences during the Stringent Response, in order to block replisome movement. This, therefore, acts as a checkpoint in chromosome elongation.

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Section: Construction Of Isogenic Dnab37 Mutant Strairis Tacking or Omentioning

confidence: 99%

A checkpoint involving RTP, the replication terminator protein, arrests replication downstream of the origin during the Stringent Response in Bacillus subtilis

Levine¹,

Autret²,

Sj³

1995

Molecular Microbiology

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“…Replication of the circular chromosome of B. subtilis occurs bidirectionally from oriC, with replication forks migrating until they fuse in a region approximately 180 from the origin. The arrest of replication forks and the coordinated termination of DNA replication is dependent upon the binding of the replication terminator protein (RTP) to its cognate DNA-binding sites known as terminator sites (Ter sites; Smith & Wake, 1988, 1989. Each Ter site comprises two pseudosymmetric binding sites for RTP: a low-affinity A site and a high-affinity B site.…”

Section: Introductionmentioning

confidence: 99%

Crystallization and preliminary X-ray diffraction analysis of theBacillus subtilisreplication termination protein in complex with the 37-base-pair TerI-binding site

et al. 2006

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The replication terminator protein (RTP) of Bacillus subtilis binds to specific DNA sequences that halt the progression of the replisome in a polar manner. These terminator complexes flank a defined region of the chromosome into which they allow replication forks to enter but not exit. Forcing the fusion of replication forks in a specific zone is thought to allow the coordination of postreplicative processes. The functional terminator complex comprises two homodimers each of 29 kDa bound to overlapping binding sites. A preparation of RTP and a 37-base-pair TerI sequence (comprising two binding sites for RTP) has been purified and crystallized. A data set to 3.9 Å resolution with 97.0% completeness and an R sym of 12% was collected from a single flash-cooled crystal using synchrotron radiation. The diffraction data are consistent with space group P622, with unit-cell parameters a = b = 118.8, c = 142.6 Å .

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Definition and polarity of action of DNA replication terminators in Bacillus subtilis

Smith

Wake

1992

Journal of Molecular Biology

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Expression of the rtp gene of Bacillus subtilis is required for replication fork arrest at the chromosome terminus

Cited by 9 publications

References 12 publications

A checkpoint involving RTP, the replication terminator protein, arrests replication downstream of the origin during the Stringent Response in Bacillus subtilis

A checkpoint involving RTP, the replication terminator protein, arrests replication downstream of the origin during the Stringent Response in Bacillus subtilis

Crystallization and preliminary X-ray diffraction analysis of theBacillus subtilisreplication termination protein in complex with the 37-base-pair TerI-binding site

Definition and polarity of action of DNA replication terminators in Bacillus subtilis

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