Expression of the seed-specific metallothionein mt4a in plant vegetative tissues increases Cu and Zn tolerance

Rodríguez‐Llorente, Ignacio D.; Pérez‐Palacios, Patricia; Doukkali, Bouchra; Caviedes, Miguel A.; Pajuelo, Eloísa

doi:10.1016/j.plantsci.2010.01.011

Cited by 47 publications

(27 citation statements)

References 26 publications

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“…The present study confirmed that transgenic tobacco plants expressing EhMT1 exhibit enhanced Cu tolerance and significantly improved growth of seedlings in the presence of excess Cu. Similar results have been reported previously: overexpression of CcMT1 from Cajanus cajan increased Cu and Cd tolerance in E. coli and Arabidopsis [38]; expression of the seed-specific mt4a gene in Arabidopsis vegetative tissues increased Cu and Zn tolerance [39]; and overexpression of BrMT1 from B. rapa in both the chloroplasts and cytosol resulted in enhanced Cd resistance in transgenic Arabidopsis [24].…”

Section: Discussionsupporting

confidence: 89%

Overexpression of Elsholtzia haichowensis metallothionein 1 (EhMT1) in tobacco plants enhances copper tolerance and accumulation in root cytoplasm and decreases hydrogen peroxide production

Xia

Yuan

et al. 2012

Journal of Hazardous Materials

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Section: Discussionsupporting

confidence: 89%

Overexpression of Elsholtzia haichowensis metallothionein 1 (EhMT1) in tobacco plants enhances copper tolerance and accumulation in root cytoplasm and decreases hydrogen peroxide production

Xia

Yuan

et al. 2012

Journal of Hazardous Materials

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“…Metallothioneins are cysteine-rich low molecular weight proteins found in plants, animals, and fungi, which are involved in metal detoxification and homeostasis in plants (Cobbett and Goldsbrough 2002). The expression of these gene products by plants is promoted by Cr and other metals (Labra et al 2006;Rodríguez-Llorente et al 2010), but their exact function is still unknown. PCs are glutathione oligomers synthesised in response to metals and they are able to form stable complexes in vivo with several metals (Leita et al 1991;Gupta et al 1995;IglesiaTuriño et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure and subcellular distribution of Cr in Iris pseudacorus L. using TEM and X-ray microanalysis

2011

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Chromium pollution of freshwater is hazardous for humans and other organisms, and places a limitation on the use of polluted water sources. Phytoremediation, the use of plants to remove pollutants from the environment, is a cost-effective, environmentally friendly approach for water decontamination. To improve the efficiency of the process, it is essential to increase the current knowledge about Cr accumulation in macrophytes. Plants of Iris pseudacorus L. were treated with Cr(III) at 0.75 mM for 5 weeks to investigate Cr localization by means of transmission electron microscopy and energy dispersive X-ray analysis. Chromium induced severe ultrastructural alterations in the rhizodermis (cell wall disorganisation, thickening, plasmolysis, and electron-dense inclusions) and rhizome parenchyma (reduced cell size, cell wall detachment, vacuolation, and opaque granules). The highest Cr contents were found in the cell walls of the cortex in the roots and in the cytoplasm and intercellular spaces of the rhizome. The Cr concentration in root tissues was in the order cortex>rhizodermis>stele, whereas in the rhizome, Cr was evenly distributed. It is proposed that root and rhizome have distinct functions in the response of I. pseudacorus to Cr. The rhizodermis limits Cr uptake by means of Si deposition and cell wall thickening. The rhizome cortex generates vacuoles and granules where Cr co-occurs with S, indicating Cr sequestration by metal-binding proteins.

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“…In Arabidopsis (Arabidopsis thaliana), constitutive expression of the seed-specific MT4 throughout the plant resulted in the accumulation of Cu but not Zn. However, the presence of MT4 in leaves and roots resulted in tolerance to both Cu and Zn when the metal ions were applied in excess (Rodríguez-Llorente et al, 2010). Simultaneous silencing of three Arabidopsis MT1 genes to transcript levels equivalent to 5% to 10% of the wild-type level induced cadmium (Cd) sensitivity (Zimeri et al, 2005).…”

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confidence: 99%

Barley Metallothioneins: MT3 and MT4 Are Localized in the Grain Aleurone Layer and Show Differential Zinc Binding

et al. 2012

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Metallothioneins (MTs) are low-molecular-weight, cysteine-rich proteins believed to play a role in cytosolic zinc (Zn) and copper (Cu) homeostasis. However, evidence for the functional properties of MTs has been hampered by methodological problems in the isolation and characterization of the proteins. Here, we document that barley (Hordeum vulgare) MT3 and MT4 proteins exist in planta and that they differ in tissue localization as well as in metal coordination chemistry. Combined transcriptional and histological analyses showed temporal and spatial correlations between transcript levels and protein abundance during grain development. MT3 was present in tissues of both maternal and filial origin throughout grain filling. In contrast, MT4 was confined to the embryo and aleurone layer, where it appeared during tissue specialization and remained until maturity. Using state-of-the-art speciation analysis by size-exclusion chromatography inductively coupled plasma mass spectrometry and electrospray ionization time-of-flight mass spectrometry on recombinant MT3 and MT4, their specificity and capacity for metal ion binding were quantified, showing a strong preferential Zn binding relative to Cu and cadmium (Cd) in MT4, which was not the case for MT3. When complementary DNAs from barley MTs were expressed in Cu-or Cd-sensitive yeast mutants, MT3 provided a much stronger complementation than did MT4. We conclude that MT3 may play a housekeeping role in metal homeostasis, while MT4 may function in Zn storage in developing and mature grains. The localization of MT4 and its discrimination against Cd make it an ideal candidate for future biofortification strategies directed toward increasing food and feed Zn concentrations.

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Expression of the seed-specific metallothionein mt4a in plant vegetative tissues increases Cu and Zn tolerance

Cited by 47 publications

References 26 publications

Overexpression of Elsholtzia haichowensis metallothionein 1 (EhMT1) in tobacco plants enhances copper tolerance and accumulation in root cytoplasm and decreases hydrogen peroxide production

Overexpression of Elsholtzia haichowensis metallothionein 1 (EhMT1) in tobacco plants enhances copper tolerance and accumulation in root cytoplasm and decreases hydrogen peroxide production

Ultrastructure and subcellular distribution of Cr in Iris pseudacorus L. using TEM and X-ray microanalysis

Barley Metallothioneins: MT3 and MT4 Are Localized in the Grain Aleurone Layer and Show Differential Zinc Binding

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