Expression of the semicarbazide-sensitive amine oxidase in articular cartilage: its role in terminal differentiation of chondrocytes in rat and human

Abstract

SSAO was expressed as an active form in rat and human cartilage. The results suggest the involvement of SSAO in rat chondrocyte terminal differentiation via a modulation of the glucose transport. In man, the increased SSAO activity detected in osteoarthritic patients may trigger hypertrophy and cartilage degeneration.

Analgesic and Anti-Inflammatory Effects of the Novel Semicarbazide-Sensitive Amine-Oxidase Inhibitor SzV-1287 in Chronic Arthritis Models of the Mouse

“…This in vivo observations were supported by the decreased metachromatic cartilage matrix production detected after LJP-1207, but not SzV-1287 treatment, in chondrocyte cultures. The cartilage damaging action of LJP-1207 in the chronically inflamed joint is not likely to be due to its SSAO inhibitory effect, since a very recently published paper reports that SSAO inhibition delayed the chondrocyte differentiation without any alteration of cell viability in vitro 38; this might be related to some other mechanisms due to its different structure. The unique features of SzV-1287 compared to other SSAO inhibitors are its COX inhibitory action, without ulcerogenic effects25, and potent dual antagonistic property at the TRPV1/TRPA1 ion channels localised on peptidergic sensory nerves39, inflammatory cells40, and chondrocytes4142.…”

New actions of an old friend: perivascular adipose tissue's adrenergic mechanisms

“…The semicarbazide sensitive amine oxidase (SSAO) has high activity in adipose tissue and thus must be considered as a potential amine metabolizer in PVAT. SSAO is involved in adipogenesis (Mercier et al, ), cell differentiation (Filip et al, ) and glucose transport (El Hadri et al, ). Elliott et al () investigated amine metabolism in rat isolated mesenteric arteries and found that metabolism of the substrates tyramine and benzylamine was carried out by SSAO and to a lesser extent MAO‐A.…”

Recent Insights into the Contribution of the Changing Hypertrophic Chondrocyte Phenotype in the Development and Progression of Osteoarthritis

“…The semicarbazide-sensitive amine oxidase (SSAO) found in the hypertrophic chondrocytes of the growth plate is known to be involved in leukocyte extravasation from the blood to the inflammation site. Additionally, it may be associated with the differentiation of chondrocytes toward a hypertrophic phenotype (Filip et al, 2016 ). In line with this, inhibition of SSAO reduced Mmp13, Alpl , and Opn expression, potentially by modulation of glucose transport in rat hypertrophic articular chondrocytes (Filip et al, 2016 ).…”

“…Additionally, it may be associated with the differentiation of chondrocytes toward a hypertrophic phenotype (Filip et al, 2016 ). In line with this, inhibition of SSAO reduced Mmp13, Alpl , and Opn expression, potentially by modulation of glucose transport in rat hypertrophic articular chondrocytes (Filip et al, 2016 ). In humans, similar expression patterns were seen for SSAO in healthy and OA articular cartilage, supporting the idea that SSAO plays a role in the process of hypertrophic differentiation of the articular chondrocyte (Filip et al, 2016 ).…”