Expression of the zga agglutinin gene in tobacco can enhance its anti-pest ability for peach-potato aphid (Myzus persica)

Zhou, Congcong; Qian, Zhongying; Qian, Ji; Xu, Hui; Chen, Lili; Luo, Xiuqing; Liang, Min; Tang, Kexuan; Xiao, Jianbo; Kai, Guoyin

doi:10.1007/s11738-011-0715-y

Cited by 11 publications

(6 citation statements)

References 24 publications

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“…Tobacco transformation was carried out as previously described [ 21 ]. Transgenic plants were selected on 1/2 MS medium supplemented with 100 μM kanamycin [ 21 – 22 ]. Genomic DNA was extracted from seedlings of transgenic plants using the modified CTAB method [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Overexpression of BcHsfA1 transcription factor from Brassica campestris improved heat tolerance of transgenic tobacco

et al. 2018

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Heat shock proteins (HSPs) are a type of conserved molecular chaperone. They exist extensively in plants and greatly contribute to their survival under heat stress. The transcriptional regulation factor heat shock factor (HSF) is thought to regulate the expression of Hsps. In this study, a novel gene designated BcHsfA1 was cloned and characterized from Brassica campestris. Bioinformatic analysis implied that BcHsfA1 belongs to the HsfA gene family and is most closely related to HsfA1 from other plants. Constitutive overexpression of BcHsfA1 significantly improved heat tolerance of tobacco seedlings by affecting physiological and biochemical processes. Moreover, the chlorophyll content of transgenic tobacco plants was significantly increased compared with wild type after heat stress, as were the activities of the important enzymatic antioxidants superoxide dismutase and peroxidase. BcHsfA1 overexpression also resulted in decreased malondialdehyde content and comparative electrical conductivity and increased soluble sugar content in transgenic tobacco plants than wild-type plants exposed to heat stress. Furthermore, we identified 11 candidate heat response genes that were significantly up-regulated in the transgenic lines exposed to heat stress. Together, these results suggested that BcHsfA1 is effective in improving heat tolerance of tobacco seedlings, which may be useful in the development of new heat-resisitant B. campestris strains by genetic engineering.

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Section: Methodsmentioning

confidence: 99%

Overexpression of BcHsfA1 transcription factor from Brassica campestris improved heat tolerance of transgenic tobacco

et al. 2018

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“…The PCR product was purified with a Gel Extraction Kit (CW2302, CWBIO), cloned into the pMD18-T vector (TaKaRa, Japan), and then sequenced. The pCAMBIA2300 + constructed before (Zhou et al 2011;Kai et al 2012) and pMD18T-BcHSP70 vectors were double digested with SpeI and NheI. The purified BcHSP70 coding region was used to replace the gus gene from pCAMBIA2300 + to generate the recombinant plasmid pCAMBIA2300 + -BcHSP70 (Supplement Fig.…”

Section: Gene Cloning and Construction Of Bchsp70 Overexpression Vectorsmentioning

confidence: 99%

“…Tobacco transformation was similarly performed according to our previously reported protocol (Zhou et al 2011). Selection of transgenic plants was performed on half strength Murashige and Skoog medium supplemented with 100 μM kanamycin and grown at 25°C under a photoperiod of 16 h light/8 h dark.…”

Section: Genetic Transformation and Pcr Assay Of Transgenic Plantsmentioning

confidence: 99%

“…Selection of transgenic plants was performed on half strength Murashige and Skoog medium supplemented with 100 μM kanamycin and grown at 25°C under a photoperiod of 16 h light/8 h dark. The well-rooted transgenic and control plants were then transferred to soil in a greenhouse under 16 h light and 8 h dark at 25°C (Zhou et al 2011;Kai et al 2012).…”

Section: Genetic Transformation and Pcr Assay Of Transgenic Plantsmentioning

confidence: 99%

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Overexpression of a Brassica campestris HSP70 in tobacco confers enhanced tolerance to heat stress

et al. 2015

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Heat shock proteins (HSPs) exist extensively in eukaryotes and are conserved molecular chaperones with important contribution to plant's survival under environmental stresses. Here, the cloning and characterization of one complementary DNA (cDNA) designated as BcHSP70 from young seedlings of Brassica campestris were reported in the present work. Bioinformatic analysis revealed that BcHSP70 belongs to the plant HSP gene family and had the closest relationship with HSP70-4 from Arabidopsis thaliana. Constitutive overexpression of BcHSP70 in tobacco obviously conferred tolerance to heat stress by affecting different plant physiological parameters. In our study, transgenic tobaccos exhibited higher chlorophyll content than wild-type control when exposed to heat stress. Superoxide dismutase (SOD) and peroxidase (POD) activities, which were helpful to decrease the damage to the membrane system, were significantly higher in transformants compared to wild-type lines. Meanwhile, lower comparative electrical conductivity and malondialdehyde (MDA) content and higher proline and soluble sugar accumulation were found in transgenic tobaccos than in wild-type lines. All these above results indicated that this isolated BcHSP70 cDNA owned the ability to improve the tolerance to heat stress in transgenic tobacco, which provides helpful information and good basement to culture new robust B. campestris variety resistant to high-temperature stress by molecular breeding in the future.

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“…The recombined plasmid was transformed into Agrobacterium tumefaciens GV3101 for transformation. The genetic transformation of tobacco was operated following the published method with minor adjustment (Zhou et al, ). The transgenic tobacco was cultivated and verified on MS medium fortified with 50 μg/ml kanamycin.…”

Section: Methodsmentioning

confidence: 99%

Molecular cloning and functional analyses of the salt‐ responsive gene KVHSP70 from Kosteletzkya virginica

et al. 2019

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Land degradation resulting from soil salinization is becoming a growing menace in almost all over the world. The degraded soil shows poor performance in water conductance and soil aeration, which threatens plant growth and development. Therefore, to discover the resistance genes and breed plant with stress tolerance are necessary. The modern molecular biotechnology makes the plant with enhanced adaption possible. To do this, KvHSP70 with 1950 bp open reading frame (ORF) from Kosteletzkya virginica was isolated, studied, and transformed into tobacco for functional study. Our research showed that it encodes a typical HSP70 protein with 649 amino acids and it could be induced by salt, high temperature, drought, and abscisic acid (ABA). The transgenic tobacco with it overexpression had higher chlorophyll content, less electrolyte leakage, lower water potential and increased proline content under salt stress. Meanwhile, the transgenic acquired superior antioxidant enzyme activities and decreased MDA content. All results highlight the significance of the new found gene in breeding salt resistant plants with molecular manipulation. Hence, K. virginica provides us with helpful information and gene resources to culture new variety adapted to salt‐affected soil and available for land rehabilitation perhaps in the future.

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Expression of the zga agglutinin gene in tobacco can enhance its anti-pest ability for peach-potato aphid (Myzus persica)

Cited by 11 publications

References 24 publications

Overexpression of BcHsfA1 transcription factor from Brassica campestris improved heat tolerance of transgenic tobacco

Overexpression of BcHsfA1 transcription factor from Brassica campestris improved heat tolerance of transgenic tobacco

Overexpression of a Brassica campestris HSP70 in tobacco confers enhanced tolerance to heat stress

Molecular cloning and functional analyses of the salt‐ responsive gene KVHSP70 from Kosteletzkya virginica

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