Expression of the α4 Integrin Subunit Gene Promoter Is Modulated by the Transcription Factor Pax-6 in Corneal Epithelial Cells

Zaniolo, Karine; Leclerc, Suzanne; Cvekl, Aleš; Vallières, Luc; Bazin, Richard; Larouche, Kathy; Guérin, Sylvain L.

doi:10.1167/iovs.03-0908

Cited by 24 publications

(17 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It seems likely that these genes are either playing direct roles in the modulation of cell adhesion or control the expression of such genes (see Collinson et al, 2004). Notably, Pax6 has been shown to modulate the expression levels of the cell adhesion molecules α4 (Zaniolo et al, 2004) and α5 (Duncan et al, 2000) integrin in eye development while N-cadherin (van Raamsdonk and Tilghman, 2000), Ndst-1 (Pan et al, 2006) and β-catenin (Smith et al, 2005) all have known roles in cell adhesion and its resulting signal processing. Although chick, human, mouse and rat, all form the lens vesicle from invagination of the lens placode, lens formation in Xenopus (Ishibashi and Yasuda, 2001) and zebrafish proceeds via delamination of the lens placodal cells (see Soules and Link, 2005).…”

Section: Proliferation and Movement Of Lens Progenitor Cellsmentioning

confidence: 99%

Genetic and epigenetic mechanisms of gene regulation during lens development

Cvekl

Duncan

2007

Progress in Retinal and Eye Research

Self Cite

141

124

View full text Add to dashboard Cite

Recent studies demonstrated a number of links between chromatin structure, gene expression, extracellular signaling and cellular differentiation during lens development. Lens progenitor cells originate from a pool of common progenitor cells, the pre-placodal region (PPR) which is formed due to a complex exchange of extracellular signals between the neural plate, naïve ectoderm and mesendoderm. A specific commitment to the lens program over alternate choices such as the formation of olfactory epithelium or the anterior pituitary is manifested by the formation of a thickened surface ectoderm, the lens placode. Mouse lens progenitor cells are characterized by the expression of a complement of lens lineage-specific transcription factors including Pax6, Six3 and Sox2, controlled by FGF and BMP signaling, followed later by c-Maf, Mab21like1, Prox1 and FoxE3. Proliferation of lens progenitors together with their morphogenetic movements results in the formation of the lens vesicle. This transient structure, comprised of lens precursor cells, is polarized with its anterior cells retaining their epithelial morphology and proliferative capacity, whereas the posterior lens precursor cells initiate terminal differentiation forming the primary lens fibers. Lens differentiation is marked by expression and accumulation of crystallins and other structural proteins. The transcriptional control of crystallin genes is characterized by the reiterative use of transcription factors required for the establishment of lens precursors in combination with more ubiquitously expressed factors (e.g. AP-1, AP-2α, CREB and USF) and recruitment of histone acetyltransferases (HATs) CBP and p300, and chromatin remodeling complexes SWI/SNF and ISWI. These studies have poised the study of lens development at the forefront of efforts to understand the connections between development, cell signaling, gene transcription and chromatin remodeling.

show abstract

Section: Proliferation and Movement Of Lens Progenitor Cellsmentioning

confidence: 99%

Genetic and epigenetic mechanisms of gene regulation during lens development

Cvekl

Duncan

2007

Progress in Retinal and Eye Research

Self Cite

141

124

View full text Add to dashboard Cite

show abstract

“…Mammalian Pax6 genes encode predominantly two forms of the Pax6 protein (see Fig. 1A), Pax6 (p46) and Pax6(5a) (p48) (Carriere et al, 1993;Richardson et al, 1995;Zaniolo et al, 2004). Additional shorter forms, p43, p33 and p32, are less abundant, mostly localized in the cytoplasm and have not been functionally characterized (Carriere et al, 1993;Jaworski et al, 1998).…”

Section: Biochemistry Of Pax6: Binding To Dna and Transcriptional Regmentioning

confidence: 99%

“…Corneal epithelium in Pax6 heterozygous is thinner owing to a reduction in the number of cell layers and exhibiting abnormal adhesive properties (Davis et al, 2003). Pax6 is also expressed in conjunctival epithelium (Koroma et al, 1997), in lacrimal gland (Makarenkova et al, 2000), in corneal stem cells (Collinson et al, 2004) and in wounded corneal epithelium (Sivak et al, 2004;Zaniolo et al, 2004).…”

Section: A B Cmentioning

confidence: 99%

“…They are likely required for tissue maintenance in adults (Zhang et al, 2001), for formation of adult corneal stem cells (Collinson et al, 2004) and for response to tissue injury (Sivak et al, 2004;Zaniolo et al, 2004). The Pax genes (Pax1 to Pax9) encode a 128 amino acid DNA-binding domain, the paired domain (PD) (see Gruss and Walther, 1992).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of gene expression by Pax6 in ocular cells: a case of tissue-preferred expression of crystallins in lens

Cvekl¹,

Yang²,

Chauhan³

et al. 2004

Int. J. Dev. Biol.

Self Cite

View full text Add to dashboard Cite

“…Overexpression of Pax6 in transfected HeLa cells induces changes in cellular shape and migration, prompts massive expression of neuronal atubulin, and establishes a postmitotic phenotype (Cartier et al, 2006). Moreover, it was shown that Pax6 regulates the transcription of cadherin 4 (Liu et al, 2001), L1-CAM (Chalepakis et al, 1994;Meech et al, 1999), NCAM (Holst et al, 1997) and a4integrin (Zaniolo et al, 2004). Pax6 target genes in mouse lens have been identified by microarray screening and include JAM-1, L1-CAM, NCAM-140, and neogenin (Chauhan et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Retinal patterning by Pax6‐dependent cell adhesion molecules

Rungger‐Brändle¹,

Ripperger

Steiner

et al. 2010

Developmental Neurobiology

View full text Add to dashboard Cite

Long-standing evidence gained from Pax6 mutant embryos pointed to an involvement of Pax6-dependent cell adhesion molecules in patterning the central nervous system and, in particular, the retina. However, direct evidence for such pathways remained elusive. We here present direct evidence that knockdown of Pax6 expression by morpholino antisense molecules in Xenopus embryos and knockdown of maternal N-cadherin (mNcad), N-cadherin (Ncad) and neural cell adhesion molecule (NCAM) produce similar phenotypes. Eye formation is reduced and retinal lamination is heavily disorganized. In Pax6 knockdown embryos, the levels of mRNAs coding for these cell adhesion molecules are markedly reduced. Overexpression of Pax6 efficiently rescues the phenotype of Pax6 knockdown embryos and restores expression of these putative target genes. Rescue of Pax6-deficiency by the putative target gene mNcad moderately rescues eye formation. The promoters of the genes coding for cell adhesion molecules contain several putative Pax6 binding sites, as determined by computer analysis. Chromatin immunoprecipitation shows that, in embryonic heads, Pax6 binds to promoter regions containing such predicted binding sites. Thus, several cell adhesion molecules are direct target genes of Pax6 and cooperate in retinal patterning.

show abstract

Expression of the α4 Integrin Subunit Gene Promoter Is Modulated by the Transcription Factor Pax-6 in Corneal Epithelial Cells

Abstract: These results provide evidence that the integrin subunit alpha4 and Pax-6 are coexpressed in RCECs and raise the possibility that Pax-6 directly regulates the expression of the alpha4 gene during corneal wound healing.

Cited by 24 publications

References 72 publications

Genetic and epigenetic mechanisms of gene regulation during lens development

Genetic and epigenetic mechanisms of gene regulation during lens development

Regulation of gene expression by Pax6 in ocular cells: a case of tissue-preferred expression of crystallins in lens

Retinal patterning by Pax6‐dependent cell adhesion molecules

Contact Info

Product

Resources

About