Expression of the β-1,3-glucanase gene bgn13.1 from Trichoderma harzianum in strawberry increases tolerance to crown rot diseases but interferes with plant growth

Mercado, José A.; Barceló, Marta; Pliego, Clara; Rey, Manuel; Caballero, José L.; Muñoz‐Blanco, Juan; Rosa, David Ruano; López‐Herrera, C. J.; Santos, Berta de los; Romero‐Muñoz, F.; Pliego‐Alfaro, Fernando

doi:10.1007/s11248-015-9895-3

Cited by 37 publications

(16 citation statements)

References 47 publications

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“…38 Expression of theb-1,3-glucanase gene from Trichoderma harzianum in Fragaria £ ananassa (strawberry) enhancesb-1,3-glucanase activity and increases tolerance to crown rot diseases. 44 Moreover, it was…”

Section: Discussionmentioning

confidence: 99%

The components of rice and watermelon root exudates and their effects on pathogenic fungus and watermelon defense

Ren

Huo

Fang

et al. 2016

Plant Signaling & Behavior

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Watermelon (Citrullus lanatus) is susceptible to wilt disease caused by the fungus Fusarium oxysporum f. sp niveum (FON). Intercropping management of watermelon/aerobic rice (Oryza sativa) alleviates watermelon wilt disease, because some unidentified component(s) in rice root exudates suppress FON sporulation and spore germination. Here, we show that the phenolic acid p-coumaric acid is present in rice root exudates only, and it inhibits FON spore germination and sporulation. We found that exogenously applied pcoumaric acid up-regulated the expression of ClPR3 in roots, as well as increased chitinase activity in leaves. Furthermore, exogenously applied p-coumaric acid increased b-1,3-glucanase activity in watermelon roots. By contrast, we found that ferulic acid was secreted by watermelon roots, but not by rice roots, and that it stimulated spore germination and sporulation of FON. Exogenous application of ferulic acid down-regulated ClPR3 expression and inhibited chitinase activity in watermelon leaves. Salicylic acid was detected in both watermelon and rice root exudates, which stimulated FON spore germination at low concentrations and suppressed spore germination at high concentrations. Exogenously applied salicylic acid did not alter ClPR3 expression, but did increase chitinase and b-1,3-glucanase activities in watermelon leaves. Together, our results show that the root exudates of phenolic acids were different between rice and watermelon, which lead to their special ecological roles on pathogenic fungus and watermelon defense.

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Section: Discussionmentioning

confidence: 99%

The components of rice and watermelon root exudates and their effects on pathogenic fungus and watermelon defense

Ren

Huo

Fang

et al. 2016

Plant Signaling & Behavior

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“…These findings were also similar to those observed in this study whereby the increased ion conductivity ( Figure 6A ) and H 2 O 2 accumulation ( Figure 6B ), the up-regulation of the HR marker genes NtHSR201 and NtHSR203 , the JA associated genes NtPR-1a/c and NtPR2 , and the ET synthesis-dependent genes NtEFE26 and NtAccdeaminase ( Figure 6D ), as well as the antimicrobial action on tobacco pathogens P. solanacearum and B. cinerea ( Figure 7 ) in ScGluD 2-expressing leaves. Similarly, Mercado et al (2015) demonstrated that transgenic strawberry plants expressing the beta-1,3-glucanase gene bgn13.1 from Trichoderma harzianum in increased tolerance to crown rot disease. Liu et al (2013) reported that the crude protein extract of transgenic tobacco lines that carrying the PpGlu gene from the fruit of Pyrus pyrifolia Nakai cv.…”

Section: Discussionmentioning

confidence: 98%

Isolation and Characterization of ScGluD2, a New Sugarcane beta-1,3-Glucanase D Family Gene Induced by Sporisorium scitamineum, ABA, H2O2, NaCl, and CdCl2 Stresses

Wang

Liu

et al. 2016

Front. Plant Sci.

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Beta-1,3-glucanases (EC 3.2.1.39), commonly known as pathogenesis-related (PR) proteins, play an important role not only in plant defense against fungal pathogens but also in plant physiological and developmental processes. However, only a limited number of sugarcane beta-1,3-glucanase genes have been isolated. In the present study, we identified and characterized a new beta-1,3-glucanase gene ScGluD2 (GenBank Acc No. KF664181) from sugarcane. An X8 domain was present at the C terminal region of ScGluD2, suggesting beta-1,3-glucan-binding function. Phylogenetic analysis showed that the predicted ScGluD2 protein was classified into subfamily D beta-1,3-glucanase. Localization of the ScGluD2 protein in the plasma membrane was determined by tagging it with green fluorescent protein. The expression of ScGluD2 was more up-regulated in sugarcane smut-resistant cultivars in the early stage (1 or 3 days) than in the susceptible ones after being challenged by the smut pathogen, revealing that ScGluD2 may be involved in defense against the invasion of Sporisorium scitamineum. Transient overexpression of ScGluD2 in Nicotiana benthamiana leaves induced a defense response and exhibited antimicrobial action on the tobacco pathogens Pseudomonas solanacearum and Botrytis cinerea, further demonstrating that ScGluD2 was related to the resistance to plant pathogens. However, the transcripts of ScGluD2 partially increased (12 h) under NaCl stress, and were steadily up-regulated from 6 to 24 h upon ABA, H2O2, and CdCl2 treatments, suggesting that ABA may be a signal molecule regulating oxidative stress and play a role in the salt and heavy metal stress-induced stimulation of ScGluD2 transcripts. Taken together, ScGluD2, a novel member of subfamily D beta-1,3-glucanase, was a stress-related gene of sugarcane involved in plant defense against smut pathogen attack and salt and heavy metal stresses.

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“…β-1 , 3-glucanase expression has been demonstrated to be directly correlated with controlling disease incidence, such as in transgenic pearl millet expressing gluc 78 [23] and transgenic canola carrying bgn 13.1 [20]; and the transgene was induced upon pathogen infection [23]. The integration of β-1 , 3-glucanase provided resistance to blast in transgenic rice [22], inhibited stem rot in transgenic conola [20] and improved tolerance to collar rot diseases in transgenic strawberry [21], suggesting efficacy of β-1 , 3-glucanase in disease control [17]. The glucanases from Trichoderma spp.…”

Section: Discussionmentioning

confidence: 99%

“…The glucanases from Trichoderma spp. usually exhibit high antifungal activity [21]; however, increased glucanase activity in transgenic strawberry, rice resulted in reduced plant vigour and yield [21, 22]. In contrast, red rot resistant transgenic sugarcane having high β-1 , 3-glucanase expression were phenotypically normal and comparable to NT sugarcane, likewise transgenic canola expressing bgn13 .…”

Section: Discussionmentioning

confidence: 99%

“…bgn 13.1 expression in Brassica napus provided enhanced glucanase activity against Sclerotinia sclerotiorum [20], and displayed tolerance against C . acutatum and Rosellinia necatrix in strawberry [21]; likewise, gluc 78 expression in rice provided resistance against Magnaporthe grisea [22], and Sclerospora graminicola in pearl millet [23]. However, there is no information in literature about expression of β-1 , 3-glucanase for resistance against fungal pathogens in sugarcane.…”

Section: Introductionmentioning

confidence: 99%

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Red rot resistant transgenic sugarcane developed through expression of β-1,3-glucanase gene

et al. 2017

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Sugarcane (Saccharum spp.) is a commercially important crop, vulnerable to fungal disease red rot caused by Colletotrichum falcatum Went. The pathogen attacks sucrose accumulating parenchyma cells of cane stalk leading to severe losses in cane yield and sugar recovery. We report development of red rot resistant transgenic sugarcane through expression of β-1,3-glucanase gene from Trichoderma spp. The transgene integration and its expression were confirmed by quantitative reverse transcription-PCR in first clonal generation raised from T0 plants revealing up to 4.4-fold higher expression, in comparison to non-transgenic sugarcane. Bioassay of transgenic plants with two virulent C. falcatum pathotypes, Cf 08 and Cf 09 causing red rot disease demonstrated that some plants were resistant to Cf 08 and moderately resistant to Cf 09. The electron micrographs of sucrose storing stalk parenchyma cells from these plants displayed characteristic sucrose-filled cells inhibiting Cf 08 hyphae and lysis of Cf 09 hyphae; in contrast, the cells of susceptible plants were sucrose depleted and prone to both the pathotypes. The transgene expression was up-regulated (up to 2.0-fold in leaves and 5.0-fold in roots) after infection, as compared to before infection in resistant plants. The transgene was successfully transmitted to second clonal generation raised from resistant transgenic plants. β-1,3-glucanase protein structural model revealed that active sites Glutamate 628 and Aspartate 569 of the catalytic domain acted as proton donor and nucleophile having role in cleaving β-1,3-glycosidic bonds and pathogen hyphal lysis.

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Expression of the β-1,3-glucanase gene bgn13.1 from Trichoderma harzianum in strawberry increases tolerance to crown rot diseases but interferes with plant growth

Cited by 37 publications

References 47 publications

The components of rice and watermelon root exudates and their effects on pathogenic fungus and watermelon defense

The components of rice and watermelon root exudates and their effects on pathogenic fungus and watermelon defense

Isolation and Characterization of ScGluD2, a New Sugarcane beta-1,3-Glucanase D Family Gene Induced by Sporisorium scitamineum, ABA, H2O2, NaCl, and CdCl2 Stresses

Red rot resistant transgenic sugarcane developed through expression of β-1,3-glucanase gene

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