1997
DOI: 10.1128/iai.65.12.5320-5325.1997
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Expression of thin aggregative fimbriae promotes interaction of Salmonella typhimurium SR-11 with mouse small intestinal epithelial cells

Abstract: The factors that mediate binding of Salmonella typhimurium to small intestinal epithelial cells have not been fully characterized. In this paper we demonstrate that elimination of production of thin aggregative fiber by a transposon insertion within the gene encoding the subunit protein of the fiber reduced binding of S. typhimurium SR-11 to a conditionally immortalized proximal small intestinal epithelial cell line established from transgenic mice. This binding defect could be overcome by transcomplementation… Show more

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Cited by 84 publications
(40 citation statements)
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“…Our experiments indicate that curli interaction with MHC-I does not in£uence phagocytic processing of curliated E. coli expressing Crl^OVA for OVA peptide presentation on K b , binding of an exogenously added OVA(257^264) to K b on the surface of pre¢xed Mx or T cell proliferation. However, this and other studies [8,44] demonstrate that curliated bacteria adhere better to eukaryotic cells, although our data suggest that this interaction is not dramatically in£uenced by MHC-I expression in the case of bacteria co-cultured with Mx. In addition, curli-pro¢cient E. coli, as well as E. coli without surface expression of curli but secreting soluble CsgA subunits were shown to induce tumor necrosis factor (TNF)-K, IL-6 and IL-8 production in human Mx [47].…”
Section: Discussioncontrasting
confidence: 90%
See 1 more Smart Citation
“…Our experiments indicate that curli interaction with MHC-I does not in£uence phagocytic processing of curliated E. coli expressing Crl^OVA for OVA peptide presentation on K b , binding of an exogenously added OVA(257^264) to K b on the surface of pre¢xed Mx or T cell proliferation. However, this and other studies [8,44] demonstrate that curliated bacteria adhere better to eukaryotic cells, although our data suggest that this interaction is not dramatically in£uenced by MHC-I expression in the case of bacteria co-cultured with Mx. In addition, curli-pro¢cient E. coli, as well as E. coli without surface expression of curli but secreting soluble CsgA subunits were shown to induce tumor necrosis factor (TNF)-K, IL-6 and IL-8 production in human Mx [47].…”
Section: Discussioncontrasting
confidence: 90%
“…Curli is thought to have a role in attachment of the bacteria to host cells. For example, it has been shown that thin, aggregative ¢mbriae mediate binding of S. typhimurium SR11 to cultured mouse small intestinal epithelial cells (H10) [44]. A binding like this might increase the infection e¤ciency and the virulence of the bacteria.…”
Section: Discussionmentioning
confidence: 99%
“…This role for SEF17 in bio¢lm formation is consistent with previous studies. In S.e., these ¢mbriae are responsible for the characteristic sti¡ colony phenotype, cell clumping, and pellicle formation on liquid medium surfaces [10,11] and cause convoluted colony morphology in Salmonella typhimurium [15]. Various pathogenic and non-pathogenic bacteria similarly use ¢mbriae to reinforce intra-bacterial cell contact including: type 4 pili of Neisseria [16,17], type 2 ¢mbriae of dental plaque-forming Actinomyces-Streptococcus coaggregates [18] and conjugative pili between bacterial mating pairs.…”
Section: Discussionmentioning
confidence: 99%
“…Various pathogenic and non-pathogenic bacteria similarly use ¢mbriae to reinforce intra-bacterial cell contact including: type 4 pili of Neisseria [16,17], type 2 ¢mbriae of dental plaque-forming Actinomyces-Streptococcus coaggregates [18] and conjugative pili between bacterial mating pairs. Like thin aggregative ¢mbriae of Salmonella, E. coli curli likely play a similar role in bio¢lm formation since curliproducing E. coli also form sti¡ colony phenotypes and cell clumping [19] or convoluted colonies [15].…”
Section: Discussionmentioning
confidence: 99%
“…Signi¢cantly, strain EAV11, a non-curliated mutant, was equally invasive. These data suggest the elaboration of curli plays no signi¢cant role in the in vivo model used, ¢ndings which contrast with those of S. typhimurium in the mouse model [21].…”
Section: Discussionmentioning
confidence: 80%