Expression of three forms of nitric oxide synthase in peripheral nerve regeneration

González‐Hernández, Tomás; Rustioni, Aldo

doi:10.1002/(sici)1097-4547(19990115)55:2<198::aid-jnr7>3.0.co;2-m

Cited by 62 publications

(33 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have shown that the three NOS isoforms are overexpressed in different cell types 2 days after lesion, reaching their greatest expression during the second week [36]. However, we did not detect the higher expression of nNOS protein compared to normal control at 2 days after SNC.…”

Section: Discussioncontrasting

confidence: 95%

“…Accumulating evidence indicated that the three NOS isoforms were induced after peripheral nerve lesion [6,36,55,56]. Deficiency of nNOS led after sciatic nerve transection to a substantial loss of dorsal root ganglia neurons and spinal cord neurons, whereas a lack of eNOS or iNOS was well tolerated without consequences for neuronal survival [4][5][6][7].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Spatiotemporal Expression of PSD-95 and nNOS After Rat Sciatic Nerve Injury

et al. 2007

View full text Add to dashboard Cite

Neuronal nitric oxide synthase (nNOS) has been implicated to influence peripheral nerve lesion and regeneration. Post-synaptic density-95 (PSD-95) is one of nNOS-anchoring proteins and plays an important role in specifying the sites of reaction of NO in nervous system. Here we established a rat sciatic nerve crush (SNC) model to examine the spatiotemporal expression of PSD-95 and nNOS. At gene levels, PSD-95 mRNA diminished shortly after crush, and significantly elevated from 2 days to 2 weeks, whereas nNOS decreased progressively post-operation, reached the valley at 1 day, and markedly up-regulated from 1 to 2 weeks after SNC. The expression of both molecules returned to the control level at 4 weeks post-injury. At protein levels, PSD-95 and nNOS underwent the similar changes as their gene expression except for a time lag during up-regulating. At their peak expression, PSD-95 co-labeled with nNOS in Schwann cells (SCs) of sciatic nerve within 0.5 mm from the lesion site, but had few colocalization in axons. In addition, the interaction between PSD-95 and nNOS enhanced significantly at 2 weeks after SNC. These results suggest a correlation of PSD-95 up-regulation with nNOS in reactive SCs of crushed sciatic nerve, which may lead to understanding the function of PSD-95 during peripheral nerve regeneration.

show abstract

Section: Discussioncontrasting

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Spatiotemporal Expression of PSD-95 and nNOS After Rat Sciatic Nerve Injury

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Scale bar=100 μm Kikuchi R et al 22 be inflammatory cells were observed in the stump and were iNOS-positive. An iNOS-positive reaction was previously shown to be induced around the ligated region in response to compressive stimulation by sciatic nerve ligation 12) , suggesting that, in the present study, transection induced inflammatory cytokines, causing an inflammatory reaction. In contrast, no eNOS was expressed in the stump, indicating that it was not involved in the nerve regeneration process.…”

Section: Discussionsupporting

confidence: 63%

Nitric Oxide Synthase (NOS) Isoform Expression after Peripheral Nerve Transection in Mice

Kikuchi

Ambe

Kon³

et al. 2018

Bull. Tokyo Dent. Coll.

View full text Add to dashboard Cite

Localization of the nitric oxide (NO)-producing enzyme, nitric oxide synthase (NOS), and its functions are currently being investigated in several tissues and organs. It has been suggested that NO is involved in nerve cell death and the development of neurodegenerative disease. The purpose of this study was to immunohistochemically investigate expression of NOS to clarify its function in the degeneration and regeneration of transected mouse sciatic nerve. Scattered neuronal NOS (nNOS)-positive Schwann cells observed on the central side of the stump on day 1 after transection showed an increase in number on day 7. None were observed at the stump on day 14, however. Expression of nNOS was observed in axons extending from the stump. The number of nNOS-positive axons increased on day 21. Inducible NOS was expressed in inflammatory cells at the stump on day 1. This positive reaction subsequently weakened by day 7, however. Endothelial NOS was expressed in blood vessels at the stump on day 7, but decreased thereafter. The results of the present study suggest that NO is involved in the proliferation and migration of Schwann cells, as well as in axon regeneration at an early stage following nerve transection.

show abstract

“…For example, nNOS is the dominant NO supplier to promote neuronal survival and recovery of DRG after peripheral nerve damage (Keilhoff et al 2003). It is also possible that NO is involved in several mechanisms related either to nerve regeneration or to the retrograde degeneration because peripheral axotomy leads to a significant increase in the small-and medium-sized NADPH-d/NOS-positive neurons in axotomized sensory ganglia (Wang et al 1996, Gonzales-Hernandez & Rustioni 1999, and to its de novo induction in MTN neurons (Varathan et al 2001). It seems likely that NADPH-d activity is plastic and can be up-regulated by a peripheral nerve injury (Vizzard et al 1995).…”

Section: Discussionmentioning

confidence: 99%

Localization of nitric oxide synthase in rat trigeminal primary afferent neurons using NADPH-diaphorase histochemistry

Stoyanova

Lazarov

2005

J Mol Hist

View full text Add to dashboard Cite

Nitric oxide (NO) is a ubiquitous gaseous neurotransmitter that has been ascribed to a large number of physiological roles in sensory neurons. It is produced by the enzyme nitric oxide synthase (NOS). To identify the NOS-containing structures of rat trigeminal primary afferent neurons, located in the trigeminal ganglion (TrG) and mesencephalic trigeminal nucleus (MTN), histochemistry to its selective marker nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) was applied in this study. In the TrG approximately half of the neuronal population was NADPH-d reactive. Strongly positive were neurons mainly of small-to-medium size. Neuronal profiles of large diameter were less intensely stained. In addition, NADPH-d-positive nerve fibers were dispersed throughout the ganglion. Nitrergic neurons were located in the caudal part and mesencephalic-pontine junction of the MTN. Most of them were large-sized pseudounipolar cells. In a more rostral aspect, the reactive psedounipolar MTN profiles gradually decreased in number and intensity of staining. There, only a fine meshwork of stained thin fibers and perisomatic terminal arborizations, and also some isolated perikarya of NADPH-d stained multipolar MTN neurons, were observed. The predominant NADPH-d localization in smaller in size TrG neurons, which are considered nociceptive, suggests that NO may play a role in the pain transmission in the rat trigeminal afferent pathways. In addition, the wide distribution of NADPH-d activity in large pseudounipolar and certain multipolar MTN neurons provides substantial evidence that NO may also participate in mediating proprioceptive information from the orofacial region. The differential expression patterns of nitrergic fibers in the TrG and MTN suggest that trigeminal sensory information processing is controlled by nitrergic input through different mechanisms.

show abstract

Expression of three forms of nitric oxide synthase in peripheral nerve regeneration

Cited by 62 publications

References 69 publications

Spatiotemporal Expression of PSD-95 and nNOS After Rat Sciatic Nerve Injury

Spatiotemporal Expression of PSD-95 and nNOS After Rat Sciatic Nerve Injury

Nitric Oxide Synthase (NOS) Isoform Expression after Peripheral Nerve Transection in Mice

Localization of nitric oxide synthase in rat trigeminal primary afferent neurons using NADPH-diaphorase histochemistry

Contact Info

Product

Resources

About