Expression of tissue transglutaminase on primary olfactory ensheathing cells cultures exposed to stress conditions

Campisi, A.; Spatuzza, Michela; Russo, Antonella; Raciti, G.; Vanella, A.; Stanzani, Stefania; Pellitteri, Rosalia

doi:10.1016/j.neures.2011.12.008

Cited by 21 publications

(71 citation statements)

References 48 publications

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“…MTT bioassay. Cell viability was monitored using MTT bioassay [74][75][76] . Results were expressed as a percentage of the control (PBS), taken as 100%, to normalize the different obtained values.…”

Section: Scientific Reports |mentioning

confidence: 99%

Synergic pro-apoptotic effects of Ferulic Acid and nanostructured lipid carrier in glioblastoma cells assessed through molecular and Delayed Luminescence studies

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Dell’Albani

Carbone

et al. 2020

Sci Rep

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Herein, we assessed the effect of Ferulic Acid (FA), a natural antioxidant with anti-cancer effect, on the human glioblastoma cells through molecular and Delayed Luminescence (DL) studies. DL, a phenomenon of ultra-week emission of optical photons, was used to monitor mitochondrial assessment. The effect of FA loaded in nanostructured lipid carriers (NLCs) was also assessed. To validate NLCs as a drug delivery system for glioblastoma treatment, particular attention was focused on their effect. We found that free FA induced a significant decrease in c-Myc and Bcl-2 expression levels accompanied by the apoptotic pathway activation. Blank NLCs, even if they did not induce cytotoxicity and caspase-3 cleavage, decreased Bcl-2, ERK1/2, c-Myc expression levels activating PARP-1 cleavage. The changes in DL intensity and kinetics highlighted a possible effect of nanoparticle matrix on mitochondria, through the involvement of the NADH pool and ROS production that, in turn, activates ERK1/2 pathways. All the effects on protein expression levels and on the activation of apoptotic pathway appeared more evident when the cells were exposed to FA loaded in NLCs. We demonstrated that the observed effects are due to a synergic pro-apoptotic influence exerted by FA, whose bioavailability increases in the glioblastoma cells, and NLCs formulation.Glioblastoma multiforme (GBM), also known as grade IV astrocytoma, represents the most prevalent and aggressive brain cancer. It is characterized by glial cells and has finger-like tentacles that infiltrate the brain, which make them very difficult to remove with surgical procedures. GBM exhibits a high level of resistance to conventional chemotherapy and radiotherapy, also due to the existence of blood-brain barrier (BBB), glioma stem cells and complex network of multiple modified signalling pathways 1 . The most frequent aberrant expression is represented by the dysregulation of extracellular signal-regulated protein kinase (ERK), which is associated with poor survival of the patients. The ERK isoforms (p42/44 or ERK1/ERK2) by interacting with specific phosphorylation substrates, play a pivotal role in the control of several cellular processes involved in proliferation, as well as activation of transcription factors, apoptosis and the control of cellular process 2,3 . In addition, the transcription factor c-Myc has been recognized as an important regulator of stem cell biology implicated with GBM malignancy and stemness 4 , as it contributes to proliferation, growth and survival of GBM stem cells 5 . GBM has been also related to the impairment of mitochondrial metabolic capacity, which leads to the alteration in energy production 6,7 and is characterized by an overexpression of Bcl-2 8 . This protein can regulate transition pores permeability of the outer mitochondrial membrane and block pro-apoptotic proteins 9 . Furthermore, it has been identified a novel www.nature.com/scientificreports www.nature.com/scientificreports/ interaction between Bcl-2 and (ADP-ribose) polymerase (PARP) 10 , t...

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Section: Scientific Reports |mentioning

confidence: 99%

Synergic pro-apoptotic effects of Ferulic Acid and nanostructured lipid carrier in glioblastoma cells assessed through molecular and Delayed Luminescence studies

Grasso

Dell’Albani

Carbone

et al. 2020

Sci Rep

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“…In preliminary experiments, a lot of the both cell cultures were exposed to different concentrations of synthesized triazole derivatives (-)5b and (-)6d [53] (0.5, 1, 5, 10, 25, 50, 100 µM) for 12, 24, 48 hr, in order to establish the optimal concentrations and their exposure times to the triazole derivates [28,62,63]. For this purpose, MTT test and morphological characterization were utilized [30].…”

Section: Treatment Of Cell Culturesmentioning

confidence: 99%

“…Cell survival analysis was performed by MTT reduction assay, evaluating mitochondrial dehydrogenase activity [28,30]. The test was conducted according to the method described by Mosmann (1983).…”

Section: Mtt Bioassaymentioning

confidence: 99%

“…Expression and localization of TG2 and caspase-3 in untreated and 5 µM triazole derivatives treated FTC-133 and 8305C culture cell lines was identified by immunocytochemical procedures [14,28,30,31]. …”

Section: Immunocytochemistrymentioning

confidence: 99%

“…In contrast, when TG2 is localized into the nuclear compartment, it phosphorylates different proteins, including retinoblastoma protein (Rb), a substrate for TG2 kinase activity [19]. Aberrant TG2 expression contributes to a variety of disease processes [20], including cancer [21][22][23][24][25], neurodegenerative diseases [26][27][28][29][30][31], autoimmune diseases, such as celiac disease [32,33], rheumatoid arthritis [34,35], tissue fibrosis [36].…”

Section: Introductionmentioning

confidence: 99%

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Expression of Tissue Transglutaminase in Human Thyroid Cancer Cell Lines: Effect of Novel Enantiopure Triazole Derivatives

Chiacchio¹

2015

Med chem

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Synthesis and Biological Activity of Triazole‐Appended N,O‐Nucleosides

et al. 2014

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1,2,3‐Triazole‐appended N,O‐nucleosides have been synthesized by an approach combining a 1,3‐dipolar cycloaddition process and an alkyne–azide click chemistry reaction. Biological assays, performed on six tumor cell lines, revealed the antiproliferative activity of the synthesized compounds. The effect was more evident in the U87MG human glioblastoma cell line. Thus, these nucleoside analogues could be promising scaffolds for the construction of new anticancer agents.

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Expression of tissue transglutaminase on primary olfactory ensheathing cells cultures exposed to stress conditions

Cited by 21 publications

References 48 publications

Synergic pro-apoptotic effects of Ferulic Acid and nanostructured lipid carrier in glioblastoma cells assessed through molecular and Delayed Luminescence studies

Synergic pro-apoptotic effects of Ferulic Acid and nanostructured lipid carrier in glioblastoma cells assessed through molecular and Delayed Luminescence studies

Expression of Tissue Transglutaminase in Human Thyroid Cancer Cell Lines: Effect of Novel Enantiopure Triazole Derivatives

Synthesis and Biological Activity of Triazole‐Appended N,O‐Nucleosides

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