Expression of tomato SlTIP2;2 enhances the tolerance to salt stress in the transgenic Arabidopsis and interacts with target proteins

Xin, Shichao; Yu, Guoliang; Sun, Linlin; Qiang, Xiaojing; Xu, Na; Cheng, Xuesen

doi:10.1007/s10265-014-0658-7

Cited by 39 publications

(22 citation statements)

References 48 publications

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“…The changes in osmotic potential are generally related to the influx or efflux of Na and K ions in the root tips. The overexpression of the SlTIP2;2 gene in the transgenic Arabidopsis significantly decreases the K efflux and Na influx in the root tips under salt stress [22]. AtLOS5 overexpression in transgenic maize enhances the net Na efflux in the roots but decreases the net K efflux [23].…”

Section: Discussionmentioning

confidence: 99%

Ectopic Expression of a Thellungiella salsuginea Aquaporin Gene, TsPIP1;1, Increased the Salt Tolerance of Rice

Wei

Qiang

Han

et al. 2018

IJMS

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Aquaporins play important regulatory roles in the transport of water and small molecules in plants. In this study, a Thellungiella salsuginea TsPIP1;1 aquaporin was transformed into Kitaake rice, and three transgenic lines were evaluated by profiling the changes of the physiological metabolism, osmotic potential, and differentially expressed genes under salt stress. The TsPIP1;1 protein contains six transmembrane domains and is localized in the cytoplasm membrane. Overexpression of the TsPIP1;1 gene not only increased the accumulation of prolines, soluble sugars and chlorophyll, but also lowered the osmotic potential and malondialdehyde content in rice under salt stress, and alleviated the amount of salt damage done to rice organs by regulating the distribution of Na/K ions, thereby promoting photosynthetic rates. Transcriptome sequencing confirmed that the differentially expressed genes that are up-regulated in rice positively respond to salt stimulus, the photosynthetic metabolic process, and the accumulation profiles of small molecules and Na/K ions. The co-expressed Rubisco and LHCA4 genes in rice were remarkably up-regulated under salt stress. This data suggests that overexpression of the TsPIP1;1 gene is involved in the regulation of water transport, the accumulation of Na/K ions, and the translocation of photosynthetic metabolites, thus conferring enhanced salt tolerance to rice.

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Section: Discussionmentioning

confidence: 99%

Ectopic Expression of a Thellungiella salsuginea Aquaporin Gene, TsPIP1;1, Increased the Salt Tolerance of Rice

Wei

Qiang

Han

et al. 2018

IJMS

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“…Tonoplast intrinsic proteins, as a major and plant-specific subfamily of AQPs, can regulate water exchange between the cytoplasm and vacuole and control cell water homeostasis involved in cellular storage, osmotic adjustment and turgor control, cell signaling and digestion, and abiotic stress tolerance (Peng et al, 2007; Wang et al, 2011; Xin et al, 2014; Song et al, 2016). Therefore, plants or crops may be conferred with increased tolerance toward different abiotic stresses through a suitable TIP -transgenic approach (Srivastava et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…For example, ectopic overexpression of TsTIP1;2 from the halophyte Thellungiella salsuginea in Arabidopsis notably increased tolerance for drought, salt, and oxidative stresses (Wang et al, 2014). Transgenic Arabidopsis plants expressing SlTIP2;2 from Solanum lycopersicum exhibited enhanced salt stress tolerance by regulating ion (including Na + and K + ) homeostasis and antioxidant enzyme activities under salt treatment (Xin et al, 2014). A high water channel activity was displayed by PgTIP1- heterologous expression in Xenopus laevis oocytes and in yeast, and a significant role in growth and development under favorable conditions and enhanced salt and drought tolerance, together with upregulated expression of the stress-related genes, was demonstrated in PgTIP1- transgenic Arabidopsis plants (Lin et al, 2007; Peng et al, 2007; Li and Cai, 2015).…”

Section: Discussionmentioning

confidence: 99%

Heterologous Expression of Panax ginseng PgTIP1 Confers Enhanced Salt Tolerance of Soybean Cotyledon Hairy Roots, Composite, and Whole Plants

Che

et al. 2017

Front. Plant Sci.

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The Panax ginseng TIP gene PgTIP1 was previously demonstrated to have high water channel activity by its heterologous expression in Xenopus laevis oocytes and in yeast; it also plays a significant role in growth of PgTIP1-transgenic Arabidopsis plants under favorable conditions and has enhanced tolerance toward salt and drought treatment. In this work, we first investigated the physiological effects of heterologous PgTIP1 expression in soybean cotyledon hairy roots or composite plants mediated by Agrobacterium rhizogenes toward enhanced salt tolerance. The PgTIP1-transgenic soybean plants mediated by the pollen tube pathway, represented by the lines N and J11, were analyzed at the physiological and molecular levels for enhanced salt tolerance. The results showed that in terms of root-specific heterologous expression, the PgTIP1-transformed soybean cotyledon hairy roots or composite plants displayed superior salt tolerance compared to the empty vector-transformed ones according to the mitigatory effects of hairy root growth reduction, drop in leaf RWC, and rise in REL under salt stress. Additionally, declines in K+ content, increases in Na+ content and Na+/K+ ratios in the hairy roots, stems, or leaves were effectively alleviated by PgTIP1-transformation, particularly the stems and leaves of composite soybean plants. At the whole plant level, PgTIP1-trasgenic soybean lines were found to possess stronger root vigor, reduced root and leaf cell membrane damage, increased SOD, POD, CAT, and APX activities, steadily increased leaf Tr, RWC, and Pn values, and smaller declines in chlorophyll and carotenoid content when exposed to salt stress compared to wild type. Moreover, the distribution patterns of Na+, K+, and Cl- in the roots, stems, and leaves of salt-stressed transgenic plants were readjusted, in that the absorbed Na+ and Cl- were mainly restricted to the roots to reduce their transport to the shoots, and the transport of root-absorbed K+ to the shoots was simultaneously promoted. PgTIP1 transformation into soybean plants enhanced the expression of some stress-related genes (GmPOD, GmAPX1, GmSOS1, and GmCLC1) in the roots and leaves under salt treatment. This indicates that the causes of enhanced salt tolerance of heterologous PgTIP1-transformed soybean are associated with the positive regulation on water relations, ion homeostasis, and ROS scavenging under salt stress both at root-specific and whole plant levels.

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“…Xin et al (2014) showed that transgenic Arabidopsis plants expressing SlTIP2;2 possessed enhanced tolerance of salinity. In addition, yeast two-hybrid and BiFC analysis in protoplasts determined the physical binding between SlTIP2;2 from tomato ( Solanum lycopersicum ) and the UDP-galactose transporter (Xin et al, 2014). …”

Section: Protein–protein Interactionsmentioning

confidence: 99%

“…The great number of AQPs in plants, the different substrates that they transport (Li et al, 2014) and the diverse forms of molecular regulation (Yaneff et al, 2015) make the study of the physiological role of AQPs a great challenge. To this complicated landscape, we have to add the recent discovery of AQP-protein and AQP-AQP interaction, which affect the regulation of aquaporin functionality (Fetter et al, 2004; Xin et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

Mutual Interactions between Aquaporins and Membrane Components

Martínez-Ballesta

Carvajal

2016

Front. Plant Sci.

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In recent years, a number of studies have been focused on the structural evaluation of protein complexes in order to get mechanistic insights into how proteins communicate at the molecular level within the cell. Specific sites of protein-aquaporin interaction have been evaluated and new forms of regulation of aquaporins described, based on these associations. Heterotetramerizations of aquaporin isoforms are considered as novel regulatory mechanisms for plasma membrane (PIPs) and tonoplast (TIPs) proteins, influencing their intrinsic permeability and trafficking dynamics in the adaptive response to changing environmental conditions. However, protein–protein interaction is an extensive theme that is difficult to tackle and new methodologies are being used to study the physical interactions involved. Bimolecular fluorescence complementation and the identification of cross-linked peptides based on tandem mass spectra, that are complementary to other methodologies such as heterologous expression, co-precipitation assays or confocal fluorescence microscopy, are discussed in this review. The chemical composition and the physical characteristics of the lipid bilayer also influence many aspects of membrane aquaporins, including their functionality. The molecular driving forces stabilizing the positions of the lipids around aquaporins could define their activity, thereby altering the conformational properties. Therefore, an integrative approach to the relevance of the membrane-aquaporin interaction to different processes related to plant cell physiology is provided. Finally, it is described how the interactions between aquaporins and copolymer matrixes or biological compounds offer an opportunity for the functional incorporation of aquaporins into new biotechnological advances.

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Expression of tomato SlTIP2;2 enhances the tolerance to salt stress in the transgenic Arabidopsis and interacts with target proteins

Cited by 39 publications

References 48 publications

Ectopic Expression of a Thellungiella salsuginea Aquaporin Gene, TsPIP1;1, Increased the Salt Tolerance of Rice

Ectopic Expression of a Thellungiella salsuginea Aquaporin Gene, TsPIP1;1, Increased the Salt Tolerance of Rice

Heterologous Expression of Panax ginseng PgTIP1 Confers Enhanced Salt Tolerance of Soybean Cotyledon Hairy Roots, Composite, and Whole Plants

Mutual Interactions between Aquaporins and Membrane Components

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