Expression of transferrin receptor and ferritin following ferumoxides–protamine sulfate labeling of cells: implications for cellular magnetic resonance imaging

Pawelczyk, Edyta; Arbab, Ali S.; Pandit, Sunil D.; Hu, Elbert; Frank, Joseph A.

doi:10.1002/nbm.1038

Cited by 116 publications

(115 citation statements)

References 36 publications

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“…Long term incubation up to 72 hours has also been investigated and shows no adverse effect upon mesenchymal stem cells . Similar results are found on ferumoxide-polylysine and ferumoxides-protamine sulfate complex toward mesenchymal stem cells (Arbab et al, 2003;Pawelczyk et al, 2006).…”

Section: Impact Of Magnetic Nanoparticles In Stem Cellsupporting

confidence: 77%

Magnetic Nanoparticles: Its Effect on Cellular Behaviour and Potential Applications

Liu¹,

Hsiao²

2012

Smart Nanoparticles Technology

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Section: Impact Of Magnetic Nanoparticles In Stem Cellsupporting

confidence: 77%

Magnetic Nanoparticles: Its Effect on Cellular Behaviour and Potential Applications

Liu¹,

Hsiao²

2012

Smart Nanoparticles Technology

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“…Bulte et al(10) used CD71 as a vehicle for intracellular monocrystalline iron oxide nanoparticle delivery in oligodendrocyte progenitor cells. Pawelczyk et al (33) found that Fe-Pro complex in vitro labeling resulted in a transient decrease in CD71 mRNA and protein levels of both HeLa and human mesenchymal stem cells. In contrast, in vitro Fe-Pro complex labeling of primary macrophages resulted in an increase in CD71 mRNA but not in protein levels.…”

Section: Discussionmentioning

confidence: 99%

In vivo leukocyte labeling with intravenous ferumoxides/protamine sulfate complex and in vitro characterization for cellular magnetic resonance imaging

Wu¹,

Muldoon²,

Várallyay³

et al. 2007

American Journal of Physiology-Cell Physiology

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Wu YJ, Muldoon LL, Varallyay C, Markwardt S, Jones RE, Neuwelt EA. In vivo leukocyte labeling with intravenous ferumoxides/protamine sulfate complex and in vitro characterization for cellular magnetic resonance imaging. Am J Physiol Cell Physiol 293: C1698-C1708, 2007. First published September 26, 2007; doi:10.1152/ajpcell.00215.2007.-Cellular labeling with ferumoxides (Feridex IV) superparamagnetic iron oxide nanoparticles can be used to monitor cells in vivo by MRI. The objective of this study was to use histology and MRI to evaluate an in vivo, as opposed to in vitro, technique for labeling of mononuclear leukocytes as a means of tracking inflammatory processes in the brain. Long-Evans rats were intravenously injected with 20 mg/kg ferumoxides, ferumoxtran-10, or ferumoxytol with or without protamine sulfate. Leukocytes and splenocytes were evaluated by cell sorting and iron histochemistry or were implanted into the brain for MRI. Injection of ferumoxides/ protamine sulfate complex IV resulted in iron labeling of leukocytes (ranging from 7.4 Ϯ 0.5% to 12.5 Ϯ 0.9% with average 9.2 Ϯ 0.8%) compared with ferumoxides (ranging from 3.9 Ϯ 0.4% to 6.3 Ϯ 0.5% with average 5.0 Ϯ 0.5%) or protamine sulfate alone (ranging from 0% to 0.9 Ϯ 0.7% with average 0.3 Ϯ 0.3%). Cell sorting analysis indicated that iron-labeled cells were enriched for cell types positive for the myelomonocytic marker (CD11b/c) and the B lymphocyte marker (CD45RA) and depleted in the T cell marker (CD3). Neither ferumoxtran-10 nor ferumoxytol with protamine sulfate labeled leukocytes. In vivo ferumoxides/protamine sulfate-loaded leukocytes and splenocytes were detected by MRI after intracerebral injection. Ferumoxides/protamine complex labeled CD45RA-positive and CD11b/c-positive leukocytes in vivo without immediate toxicity. The dose of feumoxides in this report is much higher than the approved human dose, so additional animal studies are required before this approach could be translated to the clinic. These results might provide useful information for monitoring leukocyte trafficking into the brain. leukocyte trafficking; brain; B lymphocyte; rat SUPERPARAMAGNETIC IRON OXIDE (SPIO) and ultra-small SPIO (USPIO) nanoparticle agents, consisting of an iron oxide core with a variable carbohydrate coating, are gaining use for MRI.

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“…It was shown that accumula- tion of ROS-generating substances (ATDR, iron-containing nanoparticles) by cells caused oxidation of these residues, formation of disulfide bonds and resulted in disturbances in protein conformation and interaction with mRNA causing increase of TFR1 expression [33]. Such phenomenon of sinchronous changes in FTH and TFR1 expression as a result of oxidative stress was observed by different groups of scientists on different cell lines [34,35].…”

Section: Discussionmentioning

confidence: 99%

Manifestation of Key Molecular Genetic Markers in Pharmacocorrection of Endogenous Iron Metabolism in MCF-7 and MCF-7/DDP Human Breast Cancer Cells

Чехун¹,

Lukianova²,

Demash³

et al. 2013

CellBio

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Effects of the nanocomposite and its components (magnetic fluid, cisplatin) on the level of endogenous iron exchange and the key links of genetic and epigenetic regulation of apoptotic program of sensitive and resistant MCF-7 cells were examined. We showed genetic and epigenetic mechanisms of action of nanocomposite of magnetic fluid and cisplatin. Nanocomposite caused elevation of number of cells in apoptosis in sensitive and especially resistant MCF-7 cells compared to cisplatin alone. It was proved that impact of nanocomposite on MCF-7/S and MCF-7/DDP cells caused more significant changes in expression of apoptosis regulators p53, Bcl-2 and Bax. We also suggested that changes in endogenous iron homeostasis and activation of free radical processes caused significant impact on apoptosis. Those changes included changes in methylation and expression of transferrin, its receptors, ferritin heavy and light chains (predominantly in resistant cell line), which caused activation of free radical synthesis and development of oxidative stress. We also showed that nanocomposite impact resulted into significant changes in expression of miRNA-34a and miRNA-200b, which regulated apoptosis, cell adhesion, invasion and activity of ferritin heavy chains gene. Thus, use of nanocomposite containing cisplatin and ferromagnetic as exogenous source of Fe ions caused changes of endogenous iron levels in sensitive and resistant cells allowing to increase specific activity of cytostatics and overcome factors, which promoted MDR development. Pharmacocorrection of endogenous iron metabolism allowed increasing antitumor activity of cisplatin and overcoming drug resistance.

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Expression of transferrin receptor and ferritin following ferumoxides–protamine sulfate labeling of cells: implications for cellular magnetic resonance imaging

Cited by 116 publications

References 36 publications

Magnetic Nanoparticles: Its Effect on Cellular Behaviour and Potential Applications

Magnetic Nanoparticles: Its Effect on Cellular Behaviour and Potential Applications

In vivo leukocyte labeling with intravenous ferumoxides/protamine sulfate complex and in vitro characterization for cellular magnetic resonance imaging

Manifestation of Key Molecular Genetic Markers in Pharmacocorrection of Endogenous Iron Metabolism in MCF-7 and MCF-7/DDP Human Breast Cancer Cells

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