Expression of transforming growth factors beta-1, beta 2 and beta 3 in human bladder carcinomas

Abstract

Summary We previously detected elevated transforming growth factor beta-1 (TGF-11) serum levels in patients with invasive bladder carcinomas. In this study, we therefore investigated whether elevated serum levels correlate with enhanced TGF-1 expression in human bladder tumours. mRNA levels of TGF-p1, -12 and -133 were reduced in bladder tumour tissue to 86%, 68% and 56%, respectively, of the levels in normal urothelium. On the other hand, TGF-1l protein levels were found to be higher in superficial tumours (T7-T,) (mean level of 0.153 ng mg-1) and in invasive TJ/3 tumours (mean level of 0.104 ng mg-') compared with normal urothelium (mean level of 0.065 ng mg-').Invasive T4 tumours, however, contained only low amounts of TGF-1l (mean level of 0.02 ng mg-').

Androgen receptor protein is down-regulated by basic fibroblast growth factor in prostate cancer cells

“…Total RNA was extracted from cell pellets using a guanidinium thiocyanate-acid phenol-chlorophorm (pH 4.0) extraction technique (Eder et al, 1997). Reverse transcription was performed with 500 ng of RNA in 40 µl containing finnzyme buffer [20 mM potassium phosphate, pH 7.2, 0.2 mM dithiothreitol (DTT), 0.2% Triton X-100, 5% glycerol], 0.5 mM dNTPs, 200 pmol N 6 -primers, 0.15% β-mercaptoethanol, 0.1 mg ml -1 bovine serum albumin, 39 U ribonuclease inhibitor and 10 U finnzyme AMV reverse transcriptase in a thermocycler.…”

Dose and temporal effects on gene expression profiles of urothelial cells from rats exposed to diuron

“…Tgfb3 was down-regulated in the 500 and 2500 ppm dose groups. Low levels of the Tgfb3 transcript have previously been associated with human urinary bladder tumors, suggesting a possible relationship between the lack of the tumor suppressor activity of Tgfb and urothelial neoplasia (Eder et al, 1997). Of note, these genes were not modulated after 20 weeks of diuron exposure suggesting these changes in gene expression may represent an initial perturbation of extracellular matrix homeostasis.…”

The Early Detection and Diagnosis of Bladder Cancer: A Critical Review of the Options

“…This may imply that TGF–β 1 has an inhibitory role on the cell cycle, and that low levels in more invasive tumours suggest that a lack of suppression may promote tumour progression. In another study, Eder et al [85]also showed elevated levels of this tumour marker in the urine samples of patients with bladder cancer; however, in this instance, there was no correlation with elevated TGF–β 1 levels and tumour stage.…”