Expression of tropoelastin in human periodontal ligament fibroblasts after simulation of orthodontic force

Redlich, Meir; Roos, H.; Reichenberg, E.; Zaks, Batia; Müßig, D; Baumert, Uwe; Golan, Ilan; Palmon, Aaron

doi:10.1016/j.archoralbio.2003.08.002

Cited by 40 publications

(32 citation statements)

References 19 publications

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“…Although some laboratories have demonstrated the presence of tropoelastin mRNA in vitro in the fibroblasts of human periodontal ligament (Palmon et al 2001;Redlich et al 2004), the opposite result was recently reported by Tsuruga et al (2002a,b). These latter authors demonstrated neither tropoelastin mRNA expression in the culture of human periodontal ligament fibroblasts nor soluble tropoelastin in the cell matrix layer.…”

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confidence: 65%

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Immunohistochemical Characterization of Elastic System Fibers in Rat Molar Periodontal Ligament

Sawada

Sugawara

Asai

et al. 2006

J Histochem Cytochem.

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S U M M A R Y Among elastic system fibers, oxytalan fibers are known as a ubiquitous component of the periodontal ligament, but the localization and role of elastin-containing fibers, i.e., elastic and elaunin fibers, has yet to be clarified. In this study, we immunohistochemically investigated the localization of elastin and fibrillin, major proteins of elastin-containing fibers in the periodontal ligament of rat lower first molars. At the light microscope level, distribution of elastin-positive fibers was not uniform but often concentrated in the vicinity of blood vessels in the apical region of the ligament. In contrast, fibrillin-positive fibers were more widely distributed throughout the ligament, and the pattern of their distribution was comparable to the reported distribution of oxytalan fibers. At the ultrastructural level, assemblies or bundles of abundant fibrillin-containing microfibrils were intermingled with a small amount of elastin. This observation indicated that elastin-positive fibers observed under the light microscope were elaunin fibers. No mature elastic fibers, however, were found in the ligament. These results show that the major components of elastic system fibers in the periodontal ligament of the rat mandibular first molar were oxytalan and elaunin fibers, suggesting that the elastic system fibers play a role in the mechanical protection of the vascular system.

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confidence: 65%

“…As to the origin of elastin, in vitro studies with fibroblasts of the human periodontal ligament reported expression of a tropoelastin gene (Redlich et al 2004) and secretion of elastin (Howard et al 1998). On the other hand, no expression of tropoelastin mRNA was reported in other studies (Tsuruga et al 2002a,b).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Characterization of Elastic System Fibers in Rat Molar Periodontal Ligament

Sawada

Sugawara

Asai

et al. 2006

J Histochem Cytochem.

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“…However, there has been no previous report comparing cell responses under different centrifugal forces. In this study, a centrifugal force of 600 rpm was set as low-speed and 4,800 rpm was set as highspeed rotations based on the research of Redlich et al (21)(22)(23).…”

Section: Discussionmentioning

confidence: 99%

“…where P = kg pressure per cm 2 of cells, m = mass of medium (0.002 kg), r = radius (0.09 m), rpm = revolution /min (600 or 4,800) and A = area of contact between medium and cells (9.62 cm 2 ) (20)(21)(22)(23). The cells on the dishes were then cultured continuously up to 7 days.…”

Section: Procedures Of Treating Cells With Centrifugal Forcementioning

confidence: 99%

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

et al. 2009

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Abstract:The aim of this study was to examine the effect of centrifugal force on rat bone marrow osteoblast-like cells (RBM) in vitro. RBM were seeded and cultured for 2 days. Centrifugal forces were added to the cells in the culture dishes using a centrifuge for 20 min either at 600 rpm or 4,800 rpm. The cells without centrifugation were used as control. The expression of HSP27 mRNA of both experimental groups after addition of centrifugal force for 3 hrs was significantly up-regulated compared with the control group. In the 4,800 rpm groups, the cells showed contracted shapes compared to the controls and the 600 rpm groups after treatment with centrifugal force. The maximum height of each cell in the control group was 2.00 ± 0.33 μm. The maximum height of each cell in the 600 rpm group was 1.50 ± 0.23 μm, and in the 4,800 rpm group it was 1.13 ± 0.23 μm. The proliferation ratio in the 4,800 rpm group at day 3 was significantly higher than in the other group. The expression of type I collagen and alkaline phosphatase (ALP) mRNAs in both experimental groups increased at earlier time periods compared to the control groups. ALP activity revealed no significant difference between the experimental groups and the control group. A centrifugal force of 4,800 rpm might promote the proliferation and early differentiation of rat bone marrow cells.

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“…The study of mechanically induced transcription and expression in sutures is made complex by their compound nature, consisting of a variety of extracellular matrix zones, many different cell types, and a well-developed vasculature. Essentially all of these elements are known to respond to mechanical stimuli in isolation [2][3][4][5][6][7]. Thus, although it is no surprise to find that sutures also respond [8], it is difficult to sort out which cells are responsible.…”

Section: Introductionmentioning

confidence: 99%

Mechanical Influences on Suture Development and Patency

Herring¹

2008

Frontiers of Oral Biology

132

150

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In addition to their role in skull growth, sutures are sites of flexibility between the more rigid bones. Depending on the suture, predominant loading during life may be either tensile or compressive. Loads are transmitted across sutures via collagenous fibers and a fluid-rich extracellular matrix and can be quasi-static (growth of neighboring tissues) or intermittent (mastication). The mechanical properties of sutures, while always viscoelastic, are therefore quite different for tensile versus compressive loading. The morphology of individual sutures reflects the nature of local loading, evidently by a process of developmental adaptation. In vivo or ex vivo, sutural cells respond to tensile or cyclic loading by expressing markers of proliferation and differentiation, whereas compressive loading appears to favor osteogenesis. Braincase and facial sutures exhibit similar mechanical behavior and reactions despite their different natural environments.

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Expression of tropoelastin in human periodontal ligament fibroblasts after simulation of orthodontic force

Cited by 40 publications

References 19 publications

Immunohistochemical Characterization of Elastic System Fibers in Rat Molar Periodontal Ligament

Immunohistochemical Characterization of Elastic System Fibers in Rat Molar Periodontal Ligament

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

Mechanical Influences on Suture Development and Patency

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