Expression of TSP50, SERCA2 and IL-8 in Colorectal Adenoma and Carcinoma: Correlation to Clinicopathological Factors

Abstract

Background: Colorectal cancer (CRC) is the third most common type of cancer, it is considered a genetically heterogeneous disease with different molecular pathways being involved in its initiation and progression. Testes-specific protease 50 (TSP50) gene is a member of cancer/testis antigens that encodes for threonine protease enzyme. Overexpression of TSP50 was found to enhance the progression and invasion of breast cancer and other malignant tumors. SERCA2 is widely expressed in several body tissues; its aberrant expression has been involved in many cancers. IL-8 is an inflammatory cytokine. Alongside its role in inflammation, its expression was reported to induce the migration of tumor cells.Aim: Study the expression of TSP50, SERCA2 and IL-8 in colorectal adenoma (CRA), CRC and normal colonic tissues to compare the expression of these biomarkers in relation to clinicopathological parameters and prognostic factors.Results: TSP50, SERCA2 and IL-8 expression varied between normal colonic tissues, CRA and CRC. Significant statistical association was detected between the three biomarkers’ overexpression and degree of dysplasia in CRA. Also, significant statistical relation was found between the three biomarkers’ overexpression and presence of lympho-vascular invasion, advanced TNM staging and high intra-tumoral inflammatory infiltrate. Multivariable analysis showed that the overexpression of the three biomarkers is significantly associated with worse prognosis.Conclusion: The expression of TSP50, SERCA2 and IL-8 was different between the normal tissue and neoplastic colorectal tissue on one hand and between CRA and CRC on the other. Increased expression of these biomarkers in neoplastic epithelial cells of colorectal carcinoma is associated with adverse prognostic factors and could be considered as independent prognostic factors.

Exosomal IL-8 derived from Lung Cancer and Colon Cancer cells induced adipocyte atrophy via NF-κB signaling pathway

“…IL-8 was known to increase in CRC over that in normal epithelial cells [ 29 ]; indeed, IL-8 expression was higher in the tumor and serum of patients with cachexia than in weight-stable patients [ 30 ]. The clinical data were next plotted with a KM-plot curve, dividing the patients into low- and high- expression groups based on IL-8 transcript expression in the tumor specimens obtained from lung cancer biopsies.…”

“…To assess the effects of tumor-derived EVs in CC adipocytes lipolysis, LLC/C26 cancer cell-conditioned medium (LCM and CCM) versus LCM/CCM depleted of EVs (LCM-dep and CCM-dep) were prepared as described [ 29 , 31 ]. As shown, LCM and CCM-derived EVs evoked PGC1α and UCP1 expression together with p65 phosphorylation and nuclear translocation in 3 T3-L1 adipocytes; whereas this effect was less pronounced under LCM-dep/CCM-dep treatments (Fig.…”

Expression of CXCL8 (IL-8) in the Pathogenesis of T-Cell Acute Lymphoblastic Leukemia Patients

“…This suggests that this gene plays an important role in T-ALL blood/tissue samples, making it unique and critical in leukemia development and progression compared to other genes. In recent times, research related to the identification of some novel biomarkers has become increasingly vital for the very early detection of the stages of cancers and the evaluation of chemotherapeutic strategies in personalized medicine [ 25 , 26 ]. Thus, IL-8 is highly expressed in T-ALL patients and may prove to be a good candidate biomarker for early disease detection.…”