Expression of two aldolase A mRNA species in different human and rat tissues

Schweighoffer, Fabien; Mennecier, F.; Maire, Pascal; Bachner, Lucien; Kahn, Axel

doi:10.1042/bst0140718

Cited by 5 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These genes are not highly expressed until late in embryogenesis (i.e. right before birth), after which the genes are highly up-regulated (20 -30-fold) during the first weeks of postnatal development (6). Consistently, their cognate enzymes are abundant in adult cardiac and skeletal muscle.…”

mentioning

confidence: 71%

Structure and Expression of the Murine Muscle Adenylosuccinate Synthetase Gene

Lewis

Guicherit

Datta

et al. 1996

Journal of Biological Chemistry

View full text Add to dashboard Cite

A muscle-specific isoform of adenylosuccinate synthetase (AdSS1, EC 6.3.4.4) is one of three enzymes that constitute the purine nucleotide cycle, a muscle-specific metabolic cycle. Previously, we showed that the muscle Adss1 gene was highly expressed in both skeletal muscle and heart of the adult mouse. Here we have shown that the Adss1 gene is initially activated early in embryonic development in skeletal muscle and heart precursors and is subsequently up-regulated perinatally. The earliest detectable gene expression corresponds with the establishment of the first myogenic and cardiac lineages. To allow identification of the genetic signals controlling this developmental pattern of expression, the Adss1 gene was cloned and its structure determined. Transgenic analysis has shown that 1.9 kilobase pairs of 5 flank can activate expression in skeletal muscle progenitors and direct enhanced expression to adult cardiac muscle. Sequence analysis of the promoter and 5 flanking region revealed the presence of numerous potential muscle-specific cis-regulatory elements.Studies aimed at understanding muscle gene regulation have traditionally focused on three classes of tissue-specific genes, those encoding myogenic determination factors, those encoding contractile proteins, and those encoding enzymes of energy metabolism (1, 2). The myogenic transcription factors are instrumental in all phases of myogenesis (commitment, differentiation, and maturation) and as such are expressed in a very tightly controlled temporal fashion throughout skeletal muscle and cardiac development (3, 4). Members of the second class, the contractile proteins, are considered the building blocks of muscle and cardiac fibers and accordingly do not accumulate until the onset of myotube formation during the differentiation process (5). Unlike the myogenic factors, these structural proteins (specifically the actins and myosins) progressively accumulate throughout the prenatal and postnatal phases of development, some actually reaching levels as high as 30% of adult levels at birth. Several embryonic and/or fetal isoforms disappear around the time of birth, as the levels of the adult isoforms increase during the initial period of postnatal development. The third class of muscle-specific genes encodes enzymes of muscle energy metabolism. These genes are not highly expressed until late in embryogenesis (i.e. right before birth), after which the genes are highly up-regulated (20 -30-fold) during the first weeks of postnatal development (6). Consistently, their cognate enzymes are abundant in adult cardiac and skeletal muscle. For several of these cardiac and skeletal muscle metabolic genes, it has been demonstrated that musclespecific transcripts can be detected at low levels early in development (7-12). In fact, earliest detection of muscle-specific transcripts for the ␤-enolase gene corresponds with the presence of the first muscle progenitors and the primordial cardiac tube (8). This suggests that the increased expression of the muscle metabolic genes du...

show abstract

mentioning

confidence: 71%

Structure and Expression of the Murine Muscle Adenylosuccinate Synthetase Gene

Lewis

Guicherit

Datta

et al. 1996

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…In rabbit, a qualitative modification in the pattern of aldolase A transcripts was also observed (11). Through the use of alternative promoters, aldolase A gene gives rise to multiple transcripts of different sizes (alternative first exons have different lengths) (12,16,19,38). The changes observed by Williams et al (11), before the identification of the different promoters, probably reflected a decline in M-mRNAs, the remaining transcripts corresponding to longer mRNAs controlled by the other ubiquitous promoter.…”

Section: Denervation Of Adult Transgenic Mice Induces An Early Shut-dmentioning

confidence: 99%

“…Aldolase A expression is achieved through the use of alternative promoters that have distinct tissue and developmental specificities (12)(13)(14)(15)(16)(17). The muscle-specific promoter, pM, is highly activated during postnatal muscle maturation, and in adults, pM-derived transcripts accumulate in fast twitch glycolytic muscles and to a much lower level in slow twitch muscles (14,18,19). We have previously shown that the proximal regulatory sequences (bp Ϫ310 to ϩ45 relative to the transcription start site) of the human aldolase A muscle-specific promoter are sufficient to target the expression of a chloramphenicol acetyltransferase reporter gene (pM310CAT transgene) to fast twitch muscles of transgenic mice, in a pattern mimicking its preferential expression in 2B fibers (20).…”

mentioning

confidence: 99%

Proximal Sequences of the Aldolase A Fast Muscle-specific Promoter Direct Nerve- and Activity-dependent Expression in Transgenic Mice

Spitz¹,

Vasconcelos²,

Châtelet³

et al. 1998

Journal of Biological Chemistry

View full text Add to dashboard Cite

Muscle activity is known to modulate the muscle fiber phenotype. Changes in muscle activity (normal or experimentally induced) lead to modifications of the expression status of several muscle-specific genes. However, the transcription regulatory elements involved in the adaptative response are mainly unknown. The aldolase A muscle-specific promoter, pM, is expressed in adult fast twitch muscle with a preferential expression in fast glycolytic-2B fibers. Its activity is induced during postnatal muscle maturation, suggesting a role of nerve and/or muscle activity. Indeed, denervation of gastrocnemius in newborn mice prevented the activation of the promoter in this muscle, despite the nerve-independent formation of 2B fibers. Although the nerve was necessary for pM onset during development, denervating the gastrocnemius in adults had only mild effects on pM activity. By contrast, a transgene including the pM proximal regulatory sequences that are sufficient to reproduce the 2B fiber-specific expression of the endogenous promoter was shown to be highly sensitive to both neonatal and adult denervation. Transgenes containing muscle-specific pM proximal promoter elements were used to delineate the regulatory elements involved in this response to innervation and changes in the contractile activity pattern. Nerve-and activity-dependent elements could be localized in the 130-base pair-long proximal promoter region of the human aldolase A gene.

show abstract

“…Differential screening of the library cDNA probes, obtained in our laboratory and recognizing a 3' non-coding region of rat aldolase A mRNA [6] and a coding region of rat aldolase B mRNA [7], were used to screen differentially the rat brain cDNA library.…”

Section: Preparation Of U Cdna Rat Bruin Librarymentioning

confidence: 99%

Molecular cloning and expression of rat aldolase C messenger RNA during development and hepatocarcinogenesis

et al. 1987

View full text Add to dashboard Cite

A rat brain cDNA library was screened at low stringency with an aldolase B cDNA probe corresponding to the coding sequence of the mRNA, then at high stringency with a 3' non-coding aldolase A cDNA probe. One clone, which hybridized only under the first conditions, was further characterized and used to screen the library again.Two overlapping clones, complementary to aldolase C mRNA, were obtained. They cover the 113 cdrboxyterminal coding residues and the 3' non-coding region up to the poly(A) tail. Their nucleotide sequence was determined. In the coding region the overall homology with aldolase A was 67% at the nucleotide level and 76% at the protein level. With aldolase B these values were 63% and 65% respectively. The 3' non-coding region was 380 bases long and did not exhibit any homology with the untranslated 3' extension of aldolase A and B mRNAs.Southern blot analysis indicates that probably a single aldolase C gene exists per haploid genome. Aldolase C mRNA was detected at low concentration in practically all the foetal tissues and its expression markedly and rapidly decreased after birth. In brain the concentration of aldolase C mRNA remained high and stable even after birth. Aldolase C mRNA is approximately 50-fold more abundant in brain than in foetal tissues, which are the richest in messenger RNA.In the course of azo-dye hepatocarcinogenesis the aldolase C gene is re-expressed early, with a maximum at the 4th week of carcinogenic diet, which probably corresponds to the maximal proliferation of the oval cells.

show abstract

Expression of two aldolase A mRNA species in different human and rat tissues

Cited by 5 publications

References 4 publications

Structure and Expression of the Murine Muscle Adenylosuccinate Synthetase Gene

Structure and Expression of the Murine Muscle Adenylosuccinate Synthetase Gene

Proximal Sequences of the Aldolase A Fast Muscle-specific Promoter Direct Nerve- and Activity-dependent Expression in Transgenic Mice

Molecular cloning and expression of rat aldolase C messenger RNA during development and hepatocarcinogenesis

Contact Info

Product

Resources

About