Expression of type IV collagen α1(IV)–α6(IV) polypeptides in normal and developing human kidney and in renal cell carcinomas and oncocytomas

Lohi, Jouni; Korhonen, Matti; Leivo, Ilmo; Kangas, Lauri; Tani, Taneli; Kalluri, Raghu; Miner, Jeffrey H.; Lehto, V.‐P.; Virtanen, Ismo

doi:10.1002/(sici)1097-0215(19970703)72:1<43::aid-ijc6>3.0.co;2-4

Cited by 41 publications

(23 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These 2 ␣-chains are considered as universal basement membrane components and exist throughout the body (29). In contrast, type IV collagen ␣3 and ␣4 chains are more restricted and tissue specific in their distribution and are found, for example, in fetal kidney glomeruli and also in adult kidneys but are restricted to basement membranes of glomerular Bowman's capsule and some of the distal tubules and collecting ducts (30). These general observations are consistent with our present findings on the presence and distribution of these ␣-chains in human labial salivary glands.…”

Section: Discussionsupporting

confidence: 91%

Abnormal basement membrane type IV collagen α‐chain composition in labial salivary glands in Sjögren's syndrome

Poduval¹,

Sillat²,

Virtanen³

et al. 2009

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. Sjögren's syndrome (SS) is characterized by atrophy and malfunction of the acinar cells. The aim of this study was to investigate whether type IV collagen ␣-chain composition of acinar cell compartments could be abnormal in diseased glands.Methods. Messenger RNA (mRNA) from human submandibular gland (HSG) cells, cultured with or without growth factor-depleted Matrigel, was analyzed using quantitative reverse transcription-polymerase chain reaction (RT-PCR). Labial salivary glands were analyzed using quantitative RT-PCR and immunohistochemistry.Results. HSG cells of both the ductal and acinar phenotypes synthesized all ␣-chain mRNA, in particular those of the ␣1 and ␣2 chains. Labial salivary glands (LSGs) contained ␣1/2 chains but also contained mRNA of all the other ␣-chains, although the mRNA copy numbers for the ␣3 and ␣4 chains were low, and the corresponding proteins were absent. Type IV collagen ␣1/2-chains were observed in all tubuloalveolar basement membranes. In healthy glands, ␣5 and ␣6 chains were continuous around ducts but discontinuous around acini. In SS glands, these chains were absent or patchy around the ducts and absent around the acini.Conclusion. Ductal and acinar epithelial cells are able to locally produce mRNA for all 6 different ␣-chains. Type IV collagen ␣1/2-chains seem to form the backbone in the tubuloalveolar basement membrane in salivary glands. Type IV collagen ␣3 and ␣4 chain mRNA were found in cultured salivary epithelial cells and LSG explants but were not translated to the corresponding ␣-chains in LSGs. Both ␣5 and ␣6 mRNA were observed in salivary epithelial cells and glands. In healthy glands, immunolabeling always disclosed corresponding ␣-chains around ducts, but their synthesis and/or degradation seemed to be locally regulated around acinar cells.

show abstract

Section: Discussionsupporting

confidence: 91%

Abnormal basement membrane type IV collagen α‐chain composition in labial salivary glands in Sjögren's syndrome

Poduval¹,

Sillat²,

Virtanen³

et al. 2009

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…This study investigated for the first time the immunohistochemical distribution of six collagen IV α chains in the BMs of benign and malignant odontogenic tumors based on their immunoreactivity with α(IV) chain-specific monoclonal antibodies. The expression of α(IV) chains has been reported in lung cancer [2,20], breast cancer [10,21], basal cell carcinoma [36], prostate carcinoma [4], renal cell carcinoma [14], and colorectal carcinoma [10].…”

Section: Discussionmentioning

confidence: 99%

Differential expression of collagen IV α1 to α6 chains in basement membranes of benign and malignant odontogenic tumors

et al. 2002

View full text Add to dashboard Cite

Type IV collagen, the major component of basement membrane (BM), demonstrates a stage- and position-specific distribution of its isoforms during tooth development. To determine its localization in BM of odontogenic neoplasms, immunohistochemistry using six anti-alpha(IV) chain-specific monoclonal antibodies was performed. Results disclosed that BM demonstrated an irregular alpha(IV) chain profile in malignant odontogenic tumors as compared to benign odontogenic neoplasms. No alpha3(IV) chains were detected. Expression of alpha1(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) chains was stronger in desmoplastic ( n=3) than in ordinary (n=5) ameloblastomas. The adenomatoid odontogenic tumor ( n=2) distinctly expressed these chains in BM of cribriform areas and hyaline materials (which was also alpha4(IV)-positive), but weakly around epithelial whorls/rosettes/nests and mineralized foci. These five chains also stained BM and tumor cells of ameloblastic fibroma ( n=3) and ameloblastic fibro-odontosarcoma (n=1), but not the inductive hard tissues. Ameloblastic carcinoma ( n=2) showed specific alpha1(IV)/alpha2(IV) chain loss, while primary intraosseous carcinoma ( n=1) demonstrated a discontinuous alpha1(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) staining pattern. The present results suggest that modification and remodeling of BM collagen IValpha chains occur during odontogenic neoplasms' progression.

show abstract

“…␣3-␣6(IV) chains have a more restricted tissue distribution. ␣3(IV) and ␣4(IV) are co-localized in glomerular BMs, in some tubular BMs, in kidney BMs, in alveolar BMs, and in the synaptic BM at the neuromuscular junction (Mariyama et al 1994;Lohi et al 1997). ␣5 and ␣6(IV) chains are also co-expressed in skin BMs, in some renal tubular BMs, in smooth muscle cells, and in adipocytes (Ninomiya et al 1995).…”

mentioning

confidence: 99%

Modified Basement Membrane Composition During Bronchopulmonary Tumor Progression

Catusse

Polette

Coraux

et al. 2000

J Histochem Cytochem.

View full text Add to dashboard Cite

S U M M A R Y During tumor progression, the extracellular matrix (ECM) and particularly the basement membrane (BM) appear to be dynamic structures that are not only degraded but also deposited around tumor clusters. In this study we examined by immunohistochemistry the localization of three chains of Type IV collagen ( ␣1, ␣3 and ␣5), Type VII collagen, and laminin 5 at different stages of bronchopulmonary cancers. In normal tissues, ␣1(IV) chain was detected in all BMs (bronchial, vascular, alveolar, and glandular), ␣5(IV) chain was present only in vascular BM, and laminin 5 and Type VII collagen were co-localized in bronchial and glandular BMs, whereas ␣3(IV) immunolabeling was totally absent from normal bronchi. In well-differentiated carcinomas, ␣3(IV) chain staining was found in some neosynthetized BMs interfacing the tumor cell and the stromal compartment, contrasting with the total absence of labeling in normal tissues. ␣1(IV) chain showed strong reactivity in all BM. Laminin 5 and Type VII collagen were also detected in neosynthetized BM. In poorly differentiated invasive cancers, ␣3(IV) chain and Type VII collagen were not found, whereas laminin 5 and ␣1(IV) chain persisted. The most important modifications in BM composition during tumor progression therefore appear to be the appearance of the ␣3 (IV) chain in well-differentiated carcinomas and its subsequent disappearance in poorly differentiated carcinomas, together with the loss of type VII collagen. ␣5(IV) chain distribution was restricted in vascular BM of well-and poorly differentiated carcinomas. These results show that the composition of BM is modified during the progression of bronchopulmonary tumor, emphasizing that the BM represents a dynamic element in tumor progression and has an important role in tumor cell invasiveness.

show abstract

Expression of type IV collagen α1(IV)–α6(IV) polypeptides in normal and developing human kidney and in renal cell carcinomas and oncocytomas

Cited by 41 publications

References 11 publications

Abnormal basement membrane type IV collagen α‐chain composition in labial salivary glands in Sjögren's syndrome

Abnormal basement membrane type IV collagen α‐chain composition in labial salivary glands in Sjögren's syndrome

Differential expression of collagen IV α1 to α6 chains in basement membranes of benign and malignant odontogenic tumors

Modified Basement Membrane Composition During Bronchopulmonary Tumor Progression

Contact Info

Product

Resources

About