Expression of type VI collagen mRNAs in human endometrium during the menstrual cycle and first trimester of pregnancy

Mylona, Panagiota; Kielty, Cay M.; Hoyland, Judith A.; Aplin, John D.

doi:10.1530/jrf.0.1030159

Cited by 28 publications

(8 citation statements)

References 30 publications

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“…Our observation also corroborates an earlier finding in pigs where genes for cytokeratins 8, 18 and 19 were down-regulated in the pregnant endometrium [13]. In addition, collagen VI expression decreases in human and mouse uterine tissues during the implantation [42,43]. It has been suggested that abnormally increased deposition of collagen might impair uterine function, possibly by interfering with vascularization [44] or by retarding remodeling events at implantation.…”

Section: Discussionsupporting

confidence: 91%

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Jalali

Bogacki

Dietrich

et al. 2015

Journal of Proteomics

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Section: Discussionsupporting

confidence: 91%

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Jalali

Bogacki

Dietrich

et al. 2015

Journal of Proteomics

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“…In addition, type VI collagen has been linked to the early events of chondrocyte differentiation (76), to the regulation of mesenchymal cell proliferation in vitro (77), and to ECM stabilization during development (36). It has also been hypothesized that collagen VI is important for chondrocyte proliferation and hypertrophy in cartilage (36, 78, 79). These studies in conjunction with our observation of the ubiquitous presence of type VI collagen in the growth plate (Fig.…”

Section: Discussionmentioning

confidence: 99%

Developmental and osteoarthritic changes in Col6a1‐knockout mice: Biomechanics of type VI collagen in the cartilage pericellular matrix

Alexopoulos

Youn

Bonaldo

et al. 2009

Arthritis & Rheumatism

170

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Objective. Chondrocytes, the sole cell type in articular cartilage, maintain the extracellular matrix (ECM) through a homeostatic balance of anabolic and catabolic activities that are influenced by genetic factors, soluble mediators, and biophysical factors such as mechanical stress. Chondrocytes are encapsulated by a narrow tissue region termed the "pericellular matrix" (PCM), which in normal cartilage is defined by the exclusive presence of type VI collagen. Because the PCM completely surrounds each cell, it has been hypothesized that it serves as a filter or transducer for biochemical and/or biomechanical signals from the cartilage ECM. The present study was undertaken to investigate whether lack of type VI collagen may affect the development and biomechanical function of the PCM and alter the mechanical environment of chondrocytes during joint loading.Methods. Col6a1 ؊/؊ mice, which lack type VI collagen in their organs, were generated for use in these studies. At ages 1, 3, 6, and 11 months, bone mineral density (BMD) was measured, and osteoarthritic (OA) and developmental changes in the femoral head were evaluated histomorphometrically. Mechanical properties of articular cartilage from the hip joints of 1-monthold Col6a1 ؊/؊ , Col6a1 ؉/؊ , and Col6a1 ؉/؉ mice were assessed using an electromechanical test system, and mechanical properties of the PCM were measured using the micropipette aspiration technique. Results. In Col6a1؊/؊ and Col6a1 ؉/؊ mice the PCM was structurally intact, but exhibited significantly reduced mechanical properties as compared with wildtype controls. With age, Col6a1 ؊/؊ mice showed accelerated development of OA joint degeneration, as well as other musculoskeletal abnormalities such as delayed secondary ossification and reduced BMD.Conclusion. These findings suggest that type VI collagen has an important role in regulating the physiology of the synovial joint and provide indirect evidence that alterations in the mechanical environment of chondrocytes, due to either loss of PCM properties or Col6a1 ؊/؊ -derived joint laxity, can lead to progression of OA.Articular cartilage is the tissue that lines the surfaces of diarthrodial joints and serves as the resilient, low-friction, load-bearing material for joint motion. A sparse population of cells-chondrocytes-maintains the extracellular matrix (ECM) of this tissue through a balance of anabolic and catabolic activities. The micromechanical environment of chondrocytes, in conjunction with biochemical factors (e.g., growth factors, cytokines) and genetic factors, plays an important role in cartilage homeostasis and, as a consequence, the health of the joint (1-3). Chondrocytes in articular cartilage are enclosed by a narrow region of tissue termed the "pericellular matrix" (PCM), which, together with the enclosed chondrocyte, has been termed the "chondron" (4-7). The PCM is characterized primarily as being the exclusive location of type VI collagen in normal cartilage, but proteoglycans, fibronectin, and types II and IX collagen are also pre...

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“…These matrix components may undergo compositional changes during the menstrual cycle [37, 381, especially the depletion of type VI collagen during the periimplantation period and at times of edema [39]. The prime function of type VI collagen microfibrils is to link the major elements of the extracellular matrix via their celladhesion and collagen-binding properties; thus the fact that MC tryptase or chymase effectively degrades type VI collagen [40] seems of immediate relevance to the stromal disruption and edematous changes that apparently coincide with endometrial MC degranulation.…”

Section: Fig 3 Demonstration Of Eosinophils In Human Endometrium Nmentioning

confidence: 99%

Mast Cell and Eosinophil Distribution and Activation in Human Endometrium throughout the Menstrual Cycle1

Jeziorska¹,

Salamonsen

Woolley³

1995

Biology of Reproduction

139

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Tryptase and chymase immunolocalization techniques have been used to examine the distribution, activation, and tryptase/chymase phenotype of mast cells (MCs) in 107 endometrial specimens that represented every day of the human menstrual cycle. MCs were identified in the endometrium in all stages of the menstrual cycle; similar MC numbers were observed for the functionalis, basalis, and muscularis. Extensive MC activation/degranulation, as judged by extracellular tryptase, was a common feature of the functionalis in specimens sampled just prior to and during menstruation. MC activation was also prominent in the functionalis at times coincident with recognized stromal edema. MCs of the functionalis did not contain chymase; all stained for tryptase and represent the MCT phenotype. By contrast, the basalis and muscularis showed a proportion of MCs containing both tryptase and chymase, MCTC. One important function for extracellular MC tryptase and chymase is their ability to activate precursor forms of the matrix metalloproteinases, enzymes recognized as instrumental in stromal degradation. Quantitative analysis of MC numbers, expressed relative to stromal cell numbers/mm2, indicated no major changes during the menstrual cycle, although changes in MC morphology, granule content, and activation/degranulation were recognized for specific stages. Eosinophils, detected with monoclonal antibodies EG1 and EG2, were absent from extravascular sites between Days 5 and 26 but showed local accumulations just prior to and during menstruation. Since MCs and eosinophils between them contain a variety of potent mediators, it seems likely that both cell types assume important functional roles in relation to tissue and vascular remodeling associated with endometrial physiology.

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Expression of type VI collagen mRNAs in human endometrium during the menstrual cycle and first trimester of pregnancy

Abstract: The pattern of type VI

Cited by 28 publications

References 30 publications

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Developmental and osteoarthritic changes in Col6a1‐knockout mice: Biomechanics of type VI collagen in the cartilage pericellular matrix

Mast Cell and Eosinophil Distribution and Activation in Human Endometrium throughout the Menstrual Cycle1

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