Expression of urokinase-type plasminogen activator in an experimental model of hepatocarcinoma

Nieto-Rodrı́guez, Alejandro; Hernández-Pando, Rogelio; Rodríguez‐Fragoso, Lourdes

doi:10.1016/s0300-483x(00)00404-2

Cited by 8 publications

(2 citation statements)

References 24 publications

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“…Cancer cell invasion requires degradation of the surrounding extracellular matrix, and uPA is one of such mediators of degradation. In this present study, uPA was frequently overexpressed in HCC, and this finding is in agreement with those of the previous studies (25,37). Indeed, uPA has also been regarded as a biomarker for aggressive HCC (38).…”

Section: Discussionsupporting

confidence: 94%

Evaluation of Nuclear Factor-κB, Urokinase-Type Plasminogen Activator, and HBx and Their Clinicopathological Significance in Hepatocellular Carcinoma

Chan¹,

Yau²,

Jin

et al. 2004

Clinical Cancer Research

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Purpose: Nuclear factor B (NF-B) signaling pathway is an important regulating pathway in human diseases and cancers. One of its downstream target genes is urokinase plasminogen activator (uPA), which is involved in cancer invasion and metastasis. The purpose of this study was to evaluate NF-B activation, uPA up-regulation, and hepatitis B viral X protein (HBx) expression in human hepatocellular carcinoma (HCC) and to assess their clinicopathological significance.Experimental Design: We evaluated NF-B activation, expression of uPA, and presence of HBx in 32 human HCCs. Their clinicopathological significance was assessed by correlation with the clinicopathological features. Aberrant NF-B signaling pathway and uPA up-regulation mediated by HBx were also analyzed in vitro.Results: We found that NF-B activation and uPA upregulation were frequently (56% and 59%, respectively) observed in HCCs, and particularly in HBx-positive HCCs. NF-B activation and uPA overexpression were closely associated with one another (P < 0.0001). Furthermore, both activation of NF-B and up-regulation of uPA were significantly associated with a more aggressive tumor behavior in terms of venous invasion, direct liver invasion, and absence of tumor encapsulation. In vitro, NF-B activation was induced by HBx transfection in HepG2 cells through inhibitor of nuclear factor-B kinase ␤ (IKK␤). HBx also up-regulated uPA and enhanced cell invasion synergistically with IKK␤.Conclusions: The data indicate that NF-B dysregulation and uPA overexpression may lead to a more aggressive tumor behavior in HCC. In addition, our data suggest that IKK␤ plays a critical role in the HBx-activated NF-B signaling pathway.

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Section: Discussionsupporting

confidence: 94%

Evaluation of Nuclear Factor-κB, Urokinase-Type Plasminogen Activator, and HBx and Their Clinicopathological Significance in Hepatocellular Carcinoma

Chan¹,

Yau²,

Jin

et al. 2004

Clinical Cancer Research

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“…LCR1 is a Gprotein-coupled receptor, which likely influences neuronal function at the time after maturation of brain (Wong et al, 1996). Urokinase-type plasminogen activator forms part of an important enzymatic system that degraded the extracellular matrix in process of invasion and metastasis, thereby having a strong clinical value in predicting prognosis and survival of cancer patients (Schmitt et al, 2000;Nieto-Rodriquez et al, 2001). Neutrophil gelatinase-associated lipocalin appears to be induced in epithelial cells under special conditions such as malignancy and inflammation (Nielsen et al, 1996;Cowland and Borregaard, 1997).…”

Section: Molecular Profiling Of Anticancer Effects Of 8-cladenosine Amentioning

confidence: 99%

Genome-wide expression profiling of 8-chloroadenosine- and 8-chloro-cAMP-treated human neuroblastoma cells using radioactive human cDNA microarray

Park

Choe²,

Choo³

et al. 2002

Exp Mol Med

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Previous reports raised question as to whether 8-chloro-cyclic adenosine 3,5-monophosphate (8-ClcAMP) is a prodrug for its metabolite, 8-Cl-adenosine which exerts growth inhibition in a broad spectrum of cancer cells. The present study was carried out to clarify overall cellular affects of 8-Cl-cAMP and 8-Cl-adenosine on SK-N-DZ human neuroblastoma cells by systematically characterizing gene expression using radioactive human cDNA microarray.

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Messenger RNA Profiles in Liver Injury and Stress: A Comparison of Lethal and Nonlethal Rat Models

Tygstrup

Bangert

Ott

et al. 2002

Biochemical and Biophysical Research Communications

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Expression of urokinase-type plasminogen activator in an experimental model of hepatocarcinoma

Cited by 8 publications

References 24 publications

Evaluation of Nuclear Factor-κB, Urokinase-Type Plasminogen Activator, and HBx and Their Clinicopathological Significance in Hepatocellular Carcinoma

Evaluation of Nuclear Factor-κB, Urokinase-Type Plasminogen Activator, and HBx and Their Clinicopathological Significance in Hepatocellular Carcinoma

Genome-wide expression profiling of 8-chloroadenosine- and 8-chloro-cAMP-treated human neuroblastoma cells using radioactive human cDNA microarray

Messenger RNA Profiles in Liver Injury and Stress: A Comparison of Lethal and Nonlethal Rat Models

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