Expression of UV-, blue-, long-wavelength-sensitive opsins and melatonin in extraretinal photoreceptors of the optic lobes of hawkmoths

Lampel, Jochen; Briscoe, Adriana D.; Wasserthal, Lutz T.

doi:10.1007/s00441-004-1069-1

Cited by 36 publications

(26 citation statements)

References 75 publications

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“…45. Some of our results (up-regulation of the visual perception GO category) may represent the contribution of small amounts of peripheral visual tissue adhering to the brains after dissection, but all samples were handled identically and there is evidence for brain expression of some of the genes in this GO category in insects (opsins and arrestins) (46). Alarm Pheromone Experiment.…”

Section: Methodsmentioning

confidence: 89%

Honey bee aggression supports a link between gene regulation and behavioral evolution

Alaux

Sinha²,

Hasadsri

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

246

387

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A prominent theory states that animal phenotypes arise by evolutionary changes in gene regulation, but the extent to which this theory holds true for behavioral evolution is not known. Because ''nature and nurture'' are now understood to involve hereditary and environmental influences on gene expression, we studied whether environmental influences on a behavioral phenotype, i.e., aggression, could have evolved into inherited differences via changes in gene expression. Here, with microarray analysis of honey bees, we show that aggression-related genes with inherited patterns of brain expression are also environmentally regulated. There were expression differences in the brain for hundreds of genes between the highly aggressive Africanized honey bee compared with European honey bee (EHB) subspecies. Similar results were obtained for EHB in response to exposure to alarm pheromone (which provokes aggression) and when comparing old and young bees (aggressive tendencies increase with age). There was significant overlap of the gene lists generated from these three microarray experiments. Moreover, there was statistical enrichment of several of the same cis regulatory motifs in promoters of genes on all three gene lists. Aggression shows a remarkably robust brain molecular signature regardless of whether it occurs because of inherited, age-related, or environmental (social) factors. It appears that one element in the evolution of different degrees of aggressive behavior in honey bees involved changes in regulation of genes that mediate the response to alarm pheromone.

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Section: Methodsmentioning

confidence: 89%

Honey bee aggression supports a link between gene regulation and behavioral evolution

Alaux

Sinha²,

Hasadsri

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

246

387

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show abstract

“…The adult accessory medulla is connected to the adult remnants of the larval photoreceptors, the stemmata (Lampel et al, 2005), pointing towards the larval optic neuropils as developmental origin of the accessory medulla. This larval origin was claimed for Papilio (Ichikawa, 1994); an idea originally brought up for the accessory lamina and medulla in neuropteroids (Ehnborn, 1948).…”

Section: Discussionmentioning

confidence: 99%

3D-reconstructions and virtual 4D-visualization to study metamorphic brain development in the sphinx moth Manduca sexta

Huetteroth

2010

Front. Syst. Neurosci.

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During metamorphosis, the transition from the larva to the adult, the insect brain undergoes considerable remodeling: new neurons are integrated while larval neurons are remodeled or eliminated. One well acknowledged model to study metamorphic brain development is the sphinx moth Manduca sexta. To further understand mechanisms involved in the metamorphic transition of the brain we generated a 3D standard brain based on selected brain areas of adult females and 3D reconstructed the same areas during defined stages of pupal development. Selected brain areas include for example mushroom bodies, central complex, antennal- and optic lobes. With this approach we eventually want to quantify developmental changes in neuropilar architecture, but also quantify changes in the neuronal complement and monitor the development of selected neuronal populations. Furthermore, we used a modeling software (Cinema 4D) to create a virtual 4D brain, morphing through its developmental stages. Thus the didactical advantages of 3D visualization are expanded to better comprehend complex processes of neuropil formation and remodeling during development. To obtain datasets of the M. sexta brain areas, we stained whole brains with an antiserum against the synaptic vesicle protein synapsin. Such labeled brains were then scanned with a confocal laser scanning microscope and selected neuropils were reconstructed with the 3D software AMIRA 4.1.

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“…An anti-blue opsin antibody was generated in rats against the H. erato peptide RYRAELQKRLPWMGVREAD and also immunoaffinity purified (Life Technologies, Grand Island, NY, USA). The rabbit anti-pan-UV antibody was generated against a P. glaucus peptide, CISHPKYRQELQKRMP (Lampel et al, 2005), which has a sequence similar to H. erato UVRh1 and UVRh2. In H. erato, this antibody strongly labels R1 and R2 cells that do not stain for anti-UVRh1 or anti-blue antibodies.…”

Section: Cryosectioning and Immunohistochemistrymentioning

confidence: 99%

Sexual dimorphism in the compound eye of Heliconius erato: a nymphalid butterfly with at least five spectral classes of photoreceptor

McCulloch

Osorio

Briscoe

2016

Journal of Experimental Biology

Self Cite

122

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Most butterfly families expand the number of spectrally distinct photoreceptors in their compound eye by opsin gene duplications together with lateral filter pigments; however, most nymphalid genera have limited diversity, with only three or four spectral types of photoreceptor. Here, we examined the spatial pattern of opsin expression and photoreceptor spectral sensitivities in Heliconius erato, a nymphalid with duplicate ultraviolet opsin genes, UVRh1 and UVRh2. We found that the H. erato compound eye is sexually dimorphic. Females express the two UV opsin proteins in separate photoreceptors, but males do not express UVRh1. Intracellular recordings confirmed that females have three short wavelengthsensitive photoreceptors (λ max =356, ∼390 and 470 nm), while males have two (λ max =390 and ∼470 nm). We also found two long wavelength-sensitive photoreceptors (green, λ max ∼555 nm, and red, λ max ∼600 nm), which express the same LW opsin. The red cell's shifted sensitivity is probably due to perirhabdomal filtering pigments. Sexual dimorphism of the UV-absorbing rhodopsins may reflect the females' need to discriminate conspecifics from co-mimics. Red-green color vision may be used to detect differences in red coloration on Heliconius wings, or for host-plant identification. Among nymphalids so far investigated, only H. erato is known to possess five spectral classes of photoreceptor; sexual dimorphism of the eye via suppression of one class of opsin (here UVRh1 in males) has not -to our knowledge -been reported in any animal.

show abstract

Expression of UV-, blue-, long-wavelength-sensitive opsins and melatonin in extraretinal photoreceptors of the optic lobes of hawkmoths

Cited by 36 publications

References 75 publications

Honey bee aggression supports a link between gene regulation and behavioral evolution

Honey bee aggression supports a link between gene regulation and behavioral evolution

3D-reconstructions and virtual 4D-visualization to study metamorphic brain development in the sphinx moth Manduca sexta

Sexual dimorphism in the compound eye of Heliconius erato: a nymphalid butterfly with at least five spectral classes of photoreceptor

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