Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD

Ricciardolo, Fabio Luigi Massimo; Sabatini, Federica; Sorbello, Valentina; Benedetto, Sabrina; Defilippi, Ilaria; Petecchia, Loredana; Usai, Cesare; Gnemmi, Isabella; Balbi, Bruno; Rose, Virginia De; Hacken, Nick H. T. ten; Postma, Dirkje S.; Timens, Wim; Stefano, Antonino Di

doi:10.1136/thoraxjnl-2012-202741

Cited by 30 publications

(47 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering the needs to identify biomarkers to leishmaniasis that can be used to diagnose disease, predict clinical outcome, monitor therapeutic responses and immunity [33,40], we have examined the tissue expression of some inflammatory and stress markers during cutaneous murine leishmaniosis. The predictive or diagnostic values of proteins markers have been suggested for many diseases, for example, hypoxia-inducible factor (HIF-1a) for rectal cancer [43], heme oxygenase (HO-1) for malaria [4] and bladder cancer [41], vascular endothelial growth factor (VEGF) for Sjögren's syndrome [60] and CD31/VEGF for asthma [54]. Thus, similar knowledge could help to determine the relevance of different host mediators in relation to leishmaniasis control or exacerbation and their predictive value.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical evidence of stress and inflammatory markers in mouse models of cutaneous leishmaniosis

Araújo

Giorgio

2015

Arch Dermatol Res

View full text Add to dashboard Cite

Leishmanioses are chronic parasitic diseases and host responses are associated with pro- or anti-inflammatory cytokines involved, respectively, in the control or exacerbation of infection. The relevance of other inflammatory mediators and stress markers has not been widely studied and there is a need to search for biomarkers to leishmaniasis. In this work, the stress and inflammatory molecules p38 mitogen-activated protein kinase, cyclooxygenase-2, migration inhibitory factor, macrophage inflammatory protein 2, heat shock protein 70 kDa, vascular endothelial factor (VEGF), hypoxia-inducible factors (HIF-1α and HIF-2α), heme oxygenase and galectin-3 expression were assessed immunohistochemically in self-controlled lesions in C57BL/6 mice and severe lesions in Balb/c mice infected with Leishmania amazonensis. The results indicated that the majority of molecules were expressed in the cutaneous lesions of both C57BL/6 and Balb/c mice during various phases of infection, suggesting no obvious correlation between the stress and inflammatory molecule expression and the control/exacerbation of leishmanial lesions. However, the cytokine VEGF was only detected in C57BL/6 footpad lesions and small lesions in Balb/c mice treated with antimonial pentavalent. These findings suggest that VEGF expression could be a predictive factor for murine leishmanial control, a hypothesis that should be tested in human leishmaniosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Immunohistochemical evidence of stress and inflammatory markers in mouse models of cutaneous leishmaniosis

Araújo

Giorgio

2015

Arch Dermatol Res

View full text Add to dashboard Cite

show abstract

“…17 Notch3 is reported to play an important role in regulating the alveolar epithelium. 21,22 VEGFA inhibits cell apoptosis, stimulates endothelial cell migration and proliferation, and is widely expressed in highly vascularized organs such as lungs. 20 Vascular endothelial growth factor-A (VEGFA) is a potent multifunctional cytokine that has several effects on angiogenesis and is associated with COPD.…”

Section: Introductionmentioning

confidence: 99%

miRNA‐206 regulates human pulmonary microvascular endothelial cell apoptosis via targeting in chronic obstructive pulmonary disease

Ying

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

Chronic obstructive pulmonary disease (COPD) is a leading cause of death due to tis high morbidity and mortality. microRNAs have emerged as new biomarkers for the prognosis and diagnosis of patients with COPD. In this study, we aimed to investigate the expression of microRNA‐206 (miR‐206) in lung tissues from COPD patients and to explore the regulatory role of miR‐206 in the human pulmonary microvascular endothelial cells (HPMECs). Our results showed that cigarette smoke extract (CSE) promoted cell apoptosis, increased caspase‐3 activity, and upregulated the expression of miR‐206 in HPMECs, which was significantly reversed by the miR‐206 knockdown. Transfection with miR‐206 mimics led to cell apoptosis and was closely related to changes in the protein expression levels of caspase‐3, caspase‐9, and Bcl‐2 in HPMECs. Further bioinformatics prediction analysis revealed that the 3′‐untranslated region (3′UTR) of Notch3 and vascular endothelial growth factor‐A (VEGFA) harbored miR‐206‐binding sites, and overexpression of miR‐206 repressed the luciferase activity of the vectors containing Notch3 and VEGFA 3′UTR. Overexpression of either Notch3 or VEGFA attenuated miR‐206‐induced cell apoptosis in HPMECs. More importantly, miR‐206 expression was upregulated in the lung tissues from COPD patients and was positively corrected with forced expiratory volume 1% predicted in COPD patients, while Notch3 and VEGFA mRNA levels were downregulated and were negatively correlated with the expression level of miR‐206 in the lung tissues from COPD patients. In conclusion, our results showed that miR‐206 was upregulated in COPD patients and CSE‐treated HPMECs, promoted cell apoptosis via directly targeting Notch3 and VEGFA in HPMECs.

show abstract

“…This terrific study was the winner of our gold medal in this category. Vascular remodelling has been shown in asthma and COPD, and was associated with increased expression of bradykinin receptors12 while tetrahydrobiopterin (BH4)—a cofactor of nitric oxide synthase—was identified as a potential pathway promoting vascular remodelling in IPF 13. The cytokines activin A and IL-25 were identified as potential mediators of allergen-induced airway remodelling using mouse models 14 15.…”

Section: Basic Sciencementioning

confidence: 99%

Year in review 2013: basic science and epidemiology

Cullinan¹,

Lloyd

2014

Thorax

View full text Add to dashboard Cite

Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD

Cited by 30 publications

References 34 publications

Immunohistochemical evidence of stress and inflammatory markers in mouse models of cutaneous leishmaniosis

Immunohistochemical evidence of stress and inflammatory markers in mouse models of cutaneous leishmaniosis

miRNA‐206 regulates human pulmonary microvascular endothelial cell apoptosis via targeting in chronic obstructive pulmonary disease

Year in review 2013: basic science and epidemiology

Contact Info

Product

Resources

About