Expression of Vasoactive Intestinal Peptide Receptor Messenger RNA in the Hypothalamus and Pituitary Throughout the Turkey Reproductive Cycle1

Chaiseha, Yupaporn; Youngren, Orlan M.; Halawani, Mohamed E. El

doi:10.1095/biolreprod.103.022715

Cited by 29 publications

(11 citation statements)

References 59 publications

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“…VIP mRNA and immunoreactive cell bodies have been observed in the chicken arcuate nucleus but it is not known whether VIP and AgRP mRNAs are co‐expressed. The arcuate nucleus is also a site of expression of VIP receptor (VIPR1) mRNA in the turkey ( Meleagris gallopavo ) and was particularly highly expressed in this nucleus during incubation compared to other reproductive states . It will therefore be important to determine whether VIPR1 or VIPR2 mRNAs are expressed by AgRP/NPY neurones in the chicken.…”

Section: Discussionmentioning

confidence: 99%

Hypothalamic Agouti‐Related Peptide mRNA is Elevated During Natural and Stress‐Induced Anorexia

Dunn

Wilson

D’Eath

et al. 2015

J Neuroendocrinology

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This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.As part of their natural lives, animals can undergo periods of voluntarily reduced food intake and body weight (i.e. animal anorexias) that are beneficial for survival or breeding, such as during territorial behaviour, hibernation, migration and incubation of eggs. For incubation, a change in the defended level of body weight or 'sliding set point' appears to be involved, although the neural mechanisms reponsible for this are unknown. We investigated how neuropeptide gene expression in the arcuate nucleus of the domestic chicken responded to a 60-70% voluntary reduction in food intake measured both after incubation and after an environmental stressor involving transfer to unfamiliar housing. We hypothesised that gene expression would not change in these circumstances because the reduced food intake and body weight represented a defended level in birds with free access to food. Unexpectedly, we observed increased gene expression of the orexigenic peptide agouti-related peptide (AgRP) in both incubating and transferred animals compared to controls. Also pro-opiomelanocortin (POMC) mRNA was higher in incubating hens and significantly increased 6 days after exposure to the stressor. Conversely expression of neuropeptide Y and cocaine-and amphetamine-regulated transcript gene was unchanged in both experimental situations. We conclude that AgRP expression remains sensitive to the level of energy stores during natural anorexias, which is of adaptive advantage, although its normal orexigenic effects are over-ridden by inhibitory signals. In the case of stress-induced anorexia, increased POMC may contribute to this inhibitory role, whereas, for incubation, reduced feeding may also be associated with increased expression in the hypothalamus of the anorexigenic peptide vasoactive intestinal peptide.

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Section: Discussionmentioning

confidence: 99%

Hypothalamic Agouti‐Related Peptide mRNA is Elevated During Natural and Stress‐Induced Anorexia

Dunn

Wilson

D’Eath

et al. 2015

J Neuroendocrinology

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“…The VIPR1 gene in the pituitary is involved in the regulation of reproductive activity in avian species [67]. The expression of VIP receptor mRNA in the pituitary is markedly reduced in nonphotostimulated and photorefractory turkey hens compared with laying and incubating hens [68]. In the ovaries of Huoyan geese during the egg-laying period (April–August), the rise of VIPR2 expression might be a result of the long photoperiod.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling Identifies Differentially Expressed Genes in Huoyan Goose Ovaries between the Laying Period and Ceased Period

Liu

Cao

et al. 2014

PLoS ONE

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The Huoyan goose is famous for its high egg-laying performance and is listed as a nationally protected domestic animal by the Chinese government. To elucidate the key regulatory genes involved in Huoyan goose egg laying, RNA from ovarian tissue during the ceased and laying periods was sequenced using the Illumina HiSeq 2000 sequencing platform. More than 12 million reads were produced in ceased and laying libraries that included 11,896,423 and 12,534,799 clean reads, respectively. More than 20% of the reads were matched to the reference genome, and 23% of the reads were matched to reference genes. Genes with a false discovery rate (FDR) ≤0.001 and log2ratio ≧1 or ≤−1 were characterized as differentially expressed, and 344 up-regulated and 344 down-regulated genes were classified into functional categories. Twelve genes that are mainly involved in pathways for reproduction regulation, such as steroid hormone biosynthesis, GnRH signaling pathways, oocyte meiosis, progesterone-mediated oocyte maturation, steroid biosynthesis, calcium signaling pathways, and G-protein coupled receptor signaling pathway were selected for validation by a quantitative real-time polymerase chain reaction (qRT-PCR) analysis, the qRT-PCR results are consistent with the general expression patterns of those genes from the Illumina sequencing. These data provide comprehensive gene expression information at the transcriptional level that might increase our understanding of the Huoyan goose's reproductive biology.

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“…As a receptor of VIP, VIPR is activated by VIP to give rise to secretion and release of PRL (el Halawani et al, 1990). The VIPR gene has proved to be involved in the regulation of broodiness in avian, as evidenced by the endocrine mechanism of broodiness and expression in vivo and in vitro (Rozenboim and el Halawani, 1993;Kansaku et al, 2001;Chaiseha et al, 2004). The VIPR-1 gene was expressed in the hypothalamus and pituitary, primarily in the latter, and only the differential mRNA expression of the VIPR-1 gene in the pituitary was associated with reproductive changes (You et al, 2001), suggesting that the VIPR-1 gene is an important candidate gene for egg-laying quails.…”

Section: Introductionmentioning

confidence: 99%

Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:The laying quail is a worldwide breed which exhibits high economic value. In our current study, the vasoactive intestinal peptide receptor-1 (VIPR-1) was selected as the candidate gene for identifying traits of egg production. A single nucleotide polymorphism (SNP) detection was performed in 443 individual quails, including 196 quails from the H line, 202 quails from the L line, and 45 wild quails. The SNPs were genotyped using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Two mutations (G373T, A313G) were detected in all the tested quail populations. The associated analysis showed that the SNP genotypes of the VIPR-1 gene were significantly linked with the egg weight of G373T and A313G in 398 quails. The quails with the genotype GG always exhibited the largest egg weight for the two mutations in the H and L lines. Linkage disequilibrium (LD) analysis indicated that G373T and A313G loci showed the weakest LD. Seven main diplotypes from the four main reconstructed haplotypes were observed, indicating a significant association of diplotypes with egg weight. Quails with the h1h2 (GGGT) diplotype always exhibited the smallest egg weight and largest egg number at 20 weeks of age. The overall results suggest that the alterations in quails may be linked with potential major loci or genes affecting reproductive traits.

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Expression of Vasoactive Intestinal Peptide Receptor Messenger RNA in the Hypothalamus and Pituitary Throughout the Turkey Reproductive Cycle1

Cited by 29 publications

References 59 publications

Hypothalamic Agouti‐Related Peptide mRNA is Elevated During Natural and Stress‐Induced Anorexia

Hypothalamic Agouti‐Related Peptide mRNA is Elevated During Natural and Stress‐Induced Anorexia

Transcriptome Profiling Identifies Differentially Expressed Genes in Huoyan Goose Ovaries between the Laying Period and Ceased Period

Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails

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