Expression of VEGF and CD-31 in traumatic ulcer of diabetic Wistar rats after application of Citrus limon peel essential oil

Ayuningtyas, Nurina Febriyanti; Hendarti, Hening Tuti; Soebadi, Bagus; Surboyo, Meircurius Dwi Condro; Hadi, Priyo; Ganesha, Raziv; Ernawati, Diah Savitri; Marsetyo, Riyan Iman

doi:10.1016/j.jobcr.2023.03.009

Cited by 6 publications

(6 citation statements)

References 18 publications

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“…Collagen I immunohistochemical staining results revealed that BP@CAu significantly increased collagen I deposition compared to the other four groups (Figure C), demonstrating that BP@CAu could promote collagen deposition and accelerate wound healing. Wound healing depends on the ability of new vessels to generate, and we detected the neovascularization marker CD31 to analysis angiogenesis . The immunohistochemical staining of CD31 revealed that the BP@CAu group had the highest levels of CD31 among the five groups (Figure C).…”

Section: Resultsmentioning

confidence: 99%

“…Wound healing depends on the ability of new vessels to generate, and we detected the neovascularization marker CD31 to analysis angiogenesis. 61 The immunohistochemical staining of CD31 revealed that the BP@CAu group had the highest levels of CD31 among the five groups (Figure 8C). The above results showed that BP@CAu promoted collagen deposition and neovascularization, and effectively promoted wound healing.…”

Section: Antioxidant Capacity Of Bp@cau Hydrogelsmentioning

confidence: 94%

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Dynamic Microenvironment-Adaptable Hydrogel with Photothermal Performance and ROS Scavenging for Management of Diabetic Ulcer

Jia,

Li,

Zheng

et al. 2023

ACS Appl. Mater. Interfaces

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Persistent bacterial infections and excessive oxidative stress prevent the healing of diabetic ulcers, leading to an increased disability rate. Current treatments fail to kill bacteria while simultaneously relieving oxidative stress. Herein, a dynamic microenvironment-adaptable hydrogel (BP@CAu) with photothermal performance and reactive oxygen species scavenging is presented for diabetic ulcer healing. This hydrogel prepared using a dynamic borate-ester could respond to acidity in the infection microenvironment for a controllable drug release. An excellent photothermal conversion effect was integrated in the hydrogel, which exhibited strong antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. The hydrogel attenuated intracellular oxidative stress and inflammation and promoted cell migration. In a full-thickness skin defect model of diabetic rats, the BP@CAu hydrogel contributed to the fastest wound closure, with ideal reepithelialization, granulation tissue formation, and regeneration of blood vessels. Further mechanistic studies revealed that the hydrogel relieved oxidative stress and downregulated the expression of inflammatory cytokines, resulting in dramatic therapeutic effects on diabetic wounds. Therefore, this study provides a synergistic therapeutic strategy for efficient photothermal performance and reactive oxygen species scavenging in diabetic ulcers.

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Section: Resultsmentioning

confidence: 99%

Section: Antioxidant Capacity Of Bp@cau Hydrogelsmentioning

confidence: 94%

Dynamic Microenvironment-Adaptable Hydrogel with Photothermal Performance and ROS Scavenging for Management of Diabetic Ulcer

Jia,

Li,

Zheng

et al. 2023

ACS Appl. Mater. Interfaces

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“…Table 2 shows the characteristics of the rats used in the articles reviewed. There are two types of rats used in these studies related to oral mucosal ulcer models: Wistar rats in 8 articles [15,16,18,19,22,24,27,28] and Sprague-Dawley rats in 6 articles [17,20,21,23,25,26]. The number of rat samples used varied in each article, but most articles (10 articles) did not mention the method of determining the number, while others mentioned using Animal Research Guidelines: Reporting In vivo Experiments (ARRIVE) in 2 articles and using Lemeshow's formula in 2 articles [19,23,26,28].…”

Section: Resultsmentioning

confidence: 99%

“…Most rats were housed at room temperature of 21-27 °C with a 12-hour light-dark cycle and had access to commercial rat food and water in 12 articles, and 2 articles did not mention the details. Induction methods used to obtain ulcer models were acetic acid in 7 articles [15,17,21,23,[25][26][27], biopsy punch in 2 articles [16,19], scalpel blade in 3 articles [18,22,28], thermal in 1 article [24], and phenol in 1 article [20]. The induction method using acetic acid was done in several ways: apply 15 ml of 50% acetic acid on cotton tips or round filter paper with a diameter of 3-9 mm, and then place them on the buccal and labial mucosa for 30-60 seconds, as listed in five articles [15,17,23,25,27].…”

Section: Resultsmentioning

confidence: 99%

“…A 2-3 mm-diameter burnisher tip was heated and applied without pressure for 60 seconds on the labial mucosa. The ulcer formed in a diameter of 4-5 mm within 24 h [24]. The detailed induction procedure using phenol was not described in the article, but it was mentioned that it was carried out on the buccal mucosa, and an almost uniform round ulcer was formed after 144 h [20].…”

Section: Resultsmentioning

confidence: 99%

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Oral Mucosal Ulcer Induction Methods in Rats: A Systematic Review

ERIS NURUL RAHMADHINI,

WAHYUNI

2023

Int J App Pharm

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Oral mucosal ulcers are a prevalent condition, but there are still limited drugs available to treat them. Varieties of induction techniques to obtain oral mucosal ulcer models in rats have frequently been used. This systematic review aimed to describe different approaches and to recommend the most effective method for oral mucosal ulcer induction methods in rats for anti-oral mucosal ulcer drug discovery. The PRISMA guidelines were used in the framework regarding this systematic review. The electronic databases PubMed, Science Direct, SCOPUS, and EBSCOhost-CINAHL Plus were used for article searching using specific keywords. The Risk of Bias Tool from Syrcle was used to undertake the evaluation of bias risk. Based on the analysis of 14 articles, the following findings were gathered: Wistar rats were frequently used mouse strains at an average of 8 w old and weighed between 120 and 300 g. Induction methods used to obtain ulcer models were acetic acid, biopsy punch, scalpel blade, thermal, and phenol. Acetic acid induction was the most commonly used compared to the other induction techniques. The ulcers were obtained by acetic acid identical to those that occur on the human oral mucosa and available at a reasonable price. However, the ulcer formation takes longer compared with biopsy punch and scalpel blade induction. The systematic review found that there are various methods for inducing oral ulcers in rats, with acetic acid being the recommended method to produce a suitable mucosal ulcer model in rats.

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5-Fluorouracil and Anti-EGFR antibody scaffold chitosan-stabilized Pickering emulsion: Formulations, physical characterization, in-vitro studies in NCL-H226 cells, and in-vivo investigations in Wistar rats for the augmented therapeutic effects against squamous cell carcinoma

Bhattacharya,

Shinde,

Page

et al. 2023

International Journal of Biological Macromolecules

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Expression of VEGF and CD-31 in traumatic ulcer of diabetic Wistar rats after application of Citrus limon peel essential oil

Cited by 6 publications

References 18 publications

Dynamic Microenvironment-Adaptable Hydrogel with Photothermal Performance and ROS Scavenging for Management of Diabetic Ulcer

Dynamic Microenvironment-Adaptable Hydrogel with Photothermal Performance and ROS Scavenging for Management of Diabetic Ulcer

Oral Mucosal Ulcer Induction Methods in Rats: A Systematic Review

5-Fluorouracil and Anti-EGFR antibody scaffold chitosan-stabilized Pickering emulsion: Formulations, physical characterization, in-vitro studies in NCL-H226 cells, and in-vivo investigations in Wistar rats for the augmented therapeutic effects against squamous cell carcinoma

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