Expression of VHb Improved Lipid Production in Rhodosporidium toruloides

Wang, Shuang; Kamal, Rasool; Zhang, Yue; Zhou, Renhui; Lv, Liting; Huang, Qitian; Qian, Siriguleng; Zhang, Sufang; Zhao, Zongbao K.

doi:10.3390/en13174446

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…Previous reports suggested the presence of discrepancies in cell growth and sugar consumption by the diploid and haploid strains [16,22]. To verify these observations, culture broths were analyzed for cell density (OD600) and residual glucose every 24 h. When the initial OD600 was 1.0, the diploid strain R. toruloides 4# grew faster than the haploid NP11 (Figure 1a).…”

Section: Difference In Growth Patterns Of Haploid and Diploid Strainsmentioning

confidence: 82%

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Comparative Fatty Acid Compositional Profiles of Rhodotorula toruloides Haploid and Diploid Strains under Various Storage Conditions

Zhang

Kamal

et al. 2022

Fermentation

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Microbial-based fatty acids (FAs), biofuels and oleochemicals are potential alternatives to fossil fuels and other non-renewable resources. Rhodotorula toruloides (formerly Rhodosporidium toruloides) is a basidiomycetous oleaginous yeast, and cells of the wild-type diploids can accumulate lipids to over 70 wt% on a dry cell weight basis in nutrient-limited conditions. Meanwhile, several haploid strains have been applied as hosts for producing high-value fatty acid derivatives through genetic modification and metabolic engineering. However, the differences in fatty acid compositional profiles and their stability between diploid and haploid strains remain unknown in this oleaginous yeast. Here, we grew a haploid strain R. toruloides NP11 and its parental diploid strain R. toruloides CGMCC 2.1389 (4#) under identical conditions and compared the profiles in terms of cell growth, lipid production, fatty acid compositions of lipids as well as storage stability of fatty acid methyl esters (FAMEs). It was found that lipids from R. toruloides composed of fatty acids in terms of chain length ranged from short-chain FAs (C6–C9) to very long-chain FAs (VLCFAs, C20–C24) and some odd-chain FAs (C15 and C17), while long-chain fatty acids (C14–C18) were the most abundant ones. In addition, NP11 produced a little more (1 wt%) VLCFAs than that of the diploid strain 4#. Moreover, no major changes were found for FAMEs being held under varied storage conditions, suggesting that FAMEs samples were stable and robust for fatty acid compositional analysis of microbial lipids. This work revealed the fatty acid profiles of lipids from R. toruloides haploid and diploid strains, and their stability under various storage conditions. The information is valuable for reliable assessment of fatty acid compositions of lipids from oleaginous yeasts and related microbial cell factories.

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Section: Difference In Growth Patterns Of Haploid and Diploid Strainsmentioning

confidence: 82%

“…The haploid strain NP11 can accumulate over 50 wt% lipid contents of its dry cell mass [16,23], while the diploid strain could produce more than 70 wt% of lipid contents of its dry cell mass in shake flask cultures using glucose as a carbon substrate [10,15,22].…”

Section: Lipid Accumulation Potential Of Haploid and Diploid Strainsmentioning

confidence: 99%

Comparative Fatty Acid Compositional Profiles of Rhodotorula toruloides Haploid and Diploid Strains under Various Storage Conditions

Zhang

Kamal

et al. 2022

Fermentation

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“…toruloides by overexpressing SCD1 in the overexpressing ACC1 and DAG1 strain. In another work, lipid production was increased by 116% by introducing Vitreoscilla hemoglobin (VHb) to promote oxygen supply and fulfill the high oxygen demand during lipid production . Furthermore, the engineered strain grew faster than the parental strain under conditions of high cell density.…”

Section: Metabolic Engineering Applicationsmentioning

confidence: 99%

Development and Perspective of Rhodotorula toruloides as an Efficient Cell Factory

Shi

2023

J. Agric. Food Chem.

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Rhodotorula toruloides is receiving significant attention as a novel cell factory because of its high production of lipids and carotenoids, fast growth and high cell density, as well as the ability to utilize a wide variety of substrates. These attractive traits of R. toruloides make it possible to become a low-cost producer that can be engineered for the production of various fuels and chemicals. However, the lack of understanding and genetic engineering tools impedes its metabolic engineering applications. A number of research efforts have been devoted to filling these gaps. This review focuses on recent developments in genetic engineering tools, advances in systems biology for improved understandings, and emerging engineered strains for metabolic engineering applications. Finally, future trends and barriers in developing R. toruloides as a cell factory are also discussed.

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“…Production of many metabolites are increased by the expression of VHb (Gao, Deng, Guan, Liao & Cai, 2018;Giray, 2020;Kahraman, Aytan, Giray & Özcan, 2013;Kurt, Aytan, Ozer, Ates & Geckil, 2009;Li et al, 2016b;S. Wang et al, 2020;X.…”

Section: The Vitreoscilla Hemoglobin (Vhb) Did Not Induce Vitamin a P...mentioning

confidence: 99%

Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis

Giray¹,

Pekdemir

2022

Turkish JAF Sci.Tech.

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Vitamin A is an essential micronutrient and has important functions such as vision, growth, reproduction embryogenesis, cellular differentiation, and proliferation, immune function and epithelial protector in the organism. Biotechnological production of vitamins is increasing due to their advantages and significant advances. The vitreoscilla hemoglobin (VHb) gene is extremely effective in binding oxygen and conducting it under hypoxic conditions. In this study, the production of vitamin A in E. herbicola (wild type) and its recombinant strains was investigated in LB medium and M9 medium (containing high concentrations (1%) of different carbon sources). The maximum production of vitamin A of the recombinant strain with the hemoglobin gene (vgb+) was observed in including glucose and sucrose M9 medium and their total product levels in vgb+ recombinant strain were 0.14 µg/ml and 0.1 µg/ml, respectively. The vitamin A production in the M9 medium with glucose and sucrose were 2-fold and 1.4- fold higher than that of the wild strain, respectively. The extracellular product level (0.07 µg/ml) in LB was 7-fold higher than wild strain at 48 h. These results reveal that the expression of VHb in E. herbicola in the both LB and M9 medium (containing 1% glucose and 1% sucrose, specially) increase the vitamin A production.

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Expression of VHb Improved Lipid Production in Rhodosporidium toruloides

Cited by 6 publications

References 38 publications

Comparative Fatty Acid Compositional Profiles of Rhodotorula toruloides Haploid and Diploid Strains under Various Storage Conditions

Comparative Fatty Acid Compositional Profiles of Rhodotorula toruloides Haploid and Diploid Strains under Various Storage Conditions

Development and Perspective of Rhodotorula toruloides as an Efficient Cell Factory

Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis

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