Expression of Vitreoscilla hemoglobin in Gordonia amarae enhances biosurfactant production

Doğan, İlhan; Pagilla, Krishna; Webster, D. R.; Stark, Benjamin C.

doi:10.1007/s10295-006-0097-0

Cited by 42 publications

(15 citation statements)

References 37 publications

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“…Only one study detailing the use of recombinant strains for trehalose lipid production has been reported. A recombinant Gordonia amarae was developed by insertion, stable maintenance and expression of the Vitreoscilla hemoglobin gene (vgb), resulting in enhanced production of the trehalose lipid biosurfactants in the engineered strain [42].…”

Section: Optimisation Of Productionmentioning

confidence: 99%

Production and applications of trehalose lipid biosurfactants

Franzetti

Gandolfi

Bestetti

et al. 2010

Euro J Lipid Sci & Tech

234

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Different types of trehalose containing glycolipids are known to be produced by several microorganisms belonging to the mycolates group, such as Mycobacterium, Rhodococcus, Arthrobacter, Nocardia and Gordonia. Different structures have been elucidated particularly in Rhodococcus genus. Trehalolipids have gained increased interest for their potential applications in a number of fields due to their ability to lower interfacial tension and increase pseudosolubility of hydrophobic compounds. The most widespread application is in bioremediation technologies as such compounds are known to enhance bioavailability of hydrocarbons. In comparison to other microbial glycolipids, trehalolipids have generally showed contrasting results and achievements with both cases of inhibition and enhancement of biodegradation rates. One of the important challenges regarding potential use of trehalose lipids in a variety of applications is the optimisation of their production and downstream processing. In fact, the purification of the target biological compounds by downstream processing can account for over half the production cost in many biotechnology applications. This is especially true in the case of the Rhodococcal glycolipids, which are often bound to cellular envelopes and are usually produced along with other surface active lipids. In this review, we highlight the current knowledge of trehalolipid biosurfactant applications and the latest successful strategies employed to reduce the cost of their production.

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Section: Optimisation Of Productionmentioning

confidence: 99%

Production and applications of trehalose lipid biosurfactants

Franzetti

Gandolfi

Bestetti

et al. 2010

Euro J Lipid Sci & Tech

234

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“…Other microbial responses to these two classes of stressor include the stabilization of nucleic acid structures, gene-regulating processes and membrane structures; there is a strong resemblance between the cellular responses to hydrophobic substances and hydrophilic chaotropes regardless of microbial taxon or specificity of the chemical compound (e.g. Mizushima et al, 1993;Dogan et al, 2006;Radniecki et al, 2008;Trautwein et al, 2008). Compatible solutes may also protect nucleic acid structures and those of proteins involved in nucleic acid synthesis, repair and binding (Xie and Timasheff, 1997); it has been suggested that nucleic acids represent the failure point of microbial cells under other extreme conditions (see Pitt, 1975).…”

Section: Protein Stabilizationmentioning

confidence: 99%

Hydrophobic substances induce water stress in microbial cells

Bhaganna

Volkers

Bell

et al. 2010

Microbial Biotechnology

121

185

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SummaryUbiquitous noxious hydrophobic substances, such as hydrocarbons, pesticides and diverse industrial chemicals, stress biological systems and thereby affect their ability to mediate biosphere functions like element and energy cycling vital to biosphere health. Such chemically diverse compounds may have distinct toxic activities for cellular systems; they may also share a common mechanism of stress induction mediated by their hydrophobicity. We hypothesized that the stressful effects of, and cellular adaptations to, hydrophobic stressors operate at the level of water : macromolecule interactions. Here, we present evidence that: (i) hydrocarbons reduce structural interactions within and between cellular macromolecules, (ii) organic compatible solutes – metabolites that protect against osmotic and chaotrope‐induced stresses – ameliorate this effect, (iii) toxic hydrophobic substances induce a potent form of water stress in macromolecular and cellular systems, and (iv) the stress mechanism of, and cellular responses to, hydrophobic substances are remarkably similar to those associated with chaotrope‐induced water stress. These findings suggest that it may be possible to devise new interventions for microbial processes in both natural environments and industrial reactors to expand microbial tolerance of hydrophobic substances, and hence the biotic windows for such processes.

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“…In the genus Gordonia, the most extensively studied species for the production of SACs is G. amarae (Iwahori et al 2001;Pagilla et al 2002;Dogan et al 2006). Franzettiet al (2008 reported the production of at least two types of SACs byG.…”

Section: Resultsmentioning

confidence: 99%