Expression of wild rice Porteresia coarctata PcNHX1 antiporter gene (PcNHX1) in tobacco controlled by PcNHX1 promoter (PcNHX1p) confers Na+-specific hypocotyl elongation and stem-specific Na+ accumulation in transgenic tobacco

Jegadeeson, Vidya; Kumari, Kumkum; Pulipati, Shalini; Parida, Ajay; Venkataraman, Gayatri

doi:10.1016/j.plaphy.2019.03.014

Cited by 26 publications

(18 citation statements)

References 33 publications

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“…Similarly, the expression of the wheat gene TaNHX3 increased within 24 h in roots or leaves and decreased gradually within 48 h, and transgene analysis confirmed that TaNHX3 has important functions in regulating the plant tolerance to salt stress (Lu et al, 2014). The expression of the Porteresia coarctata gene PcNHX1 in roots increased gradually within 24 h of salt treatment and then decreased gradually from 36 to 48 h and transgenic tobacco PcNHX1 seedlings show growth advantage in increasing NaCl (Jegadeeson et al, 2019). Interestingly, during root treatment (Figure 8), the expression of GhNHX_5, GhNHX_17, and GhNHX_20 decreased rapidly from 0 to 3 h under high-salt conditions (200 mM NaCl) and increased with increasing salt treatment time in contrast to the relatively low expression at 0 h under low-salt conditions, which may be related to regulation of the salt tolerance of upland cotton TM-1 with different concentrations of salt treatment.…”

Section: Expression Analysis Of Ghnhx Genes Under Multiple Stressesmentioning

confidence: 79%

“…NHX genes are involved in the salt stress response, cell expansion, membrane vesicle trafficking, and pH homeostasis (Dragwidge et al, 2019;Jegadeeson et al, 2019). Developmental disorders and cell division abnormalities in embryo and root tissues were observed after double knockout of AtNHX5 and AtNHX6 in A. thaliana (Dragwidge et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Expression Analysis of the NHX (Sodium/Hydrogen Antiporter) Gene Family in Cotton

Wei

et al. 2020

Front. Genet.

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The sodium/hydrogen antiporter (NHX) gene family with the Na + /H + exchange protein domain is a transporter of sodium and hydrogen ions and plays an important role in the response of plants to salt stress. Studying the response of cotton to salt stress through comprehensive identification and analysis of NHX genes in several species and their roles in salt tolerance mechanisms is of great significance. In this study, 23, 24, 12, and 12 NHX genes were identified from Gossypium hirsutum (Gh), G. barbadense, G. arboreum and G. raimondii, respectively. Phylogenetic analysis showed that these genes were mainly divided into three clades with significant subcellular localization, namely, endosome (Endo-class), plasma membrane (PM-class) and vacuole (Vac-class). By analyzing the structure of NHX genes and proteins, each branch of the NHX gene family was found to be structurally conserved, and collinearity analysis showed that NHX genes were mainly expressed through whole genome and segmental duplication. The non-synonymous (Ka)/synonymous (Ks) values showed that the NHX gene family experienced strong purifying selection during long-term evolution. Cis-acting element analysis showed that the NHX gene family may be related to the regulation of abscisic acid (ABA) and methyl jasmonate (MeJA) hormones. Additionally, transcriptomic data analysis and qRT-PCR showed that GhNHXs exhibited different expression patterns in each tissue and under different salinities. These results provide an important reference for us to further understand and analyze the molecular regulation mechanism of cotton NHX genes.

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Section: Expression Analysis Of Ghnhx Genes Under Multiple Stressesmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the NHX (Sodium/Hydrogen Antiporter) Gene Family in Cotton

Wei

et al. 2020

Front. Genet.

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“…Interestingly, more TaNHX3 was detected in salt-tolerant cultivar “Ji7369” compared with salt-sensitive cultivars “Shimai12” and “Ji-Shi-3” [59]. Additionally, in Porteresia coarctata , the mRNA levels of PcNHX1 in roots increased gradually up to 24 h and subsequently reduced to half of the initial level from 36 to 48 h after salt treatment and also upon salt withdrawal [61]. These results further confirmed that Vac-class NHXs play critical roles in the salt tolerance of plants.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Na+/H+ Antiporter (NHX) Genes in Sugar Beet (Beta vulgaris L.) and Their Regulated Expression under Salt Stress

Wang

2019

Genes

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Salinity is one of the major environment factors that limits the growth of plants and the productivity of crops worldwide. It has been shown that Na+ transporters play a central role in salt tolerance and development of plants. The objective of this study was to identify Na+/H+ antiporter (NHX) genes and investigate their expression patterns in sugar beet (Beta vulgaris L.) subjected to various concentrations of NaCl. A total of five putative NHX genes were identified and distributed on four chromosomes in sugar beet. Phylogenetic analysis revealed that these BvNHX genes are grouped into three major classes, viz Vac- (BvNHX1, -2 and -3), Endo- (BvNHX4), and PM-class NHX (BvNHX5/BvSOS1), and within each class the exon/intron structures are conserved. The amiloride-binding site is found in TM3 at N-terminus of Vac-class NHX proteins. Protein-protein interaction (PPI) prediction suggested that only BvNHX5 putatively interacts with calcineurin B-like proteins (CBL) and CBL-interacting protein kinases (CIPK), implying it might be the primary NHX involved in CBL-CIPK pathway under saline condition. It was also found that BvNHX5 contains one abscisic acid (ABA)-responsive element (ABRE), suggesting that BvNHX5 might be involved in ABA signal responsiveness. Additionally, the qRT-PCR analysis showed that all the BvNHX genes in both roots and leaves are significantly up-regulated by salt, and the transcription levels under high salinity are significantly higher than those under either low or moderate salinity. Taken together, this work gives a detailed overview of the BvNHX genes and their expression patterns under salt stress. Our findings also provide useful information for elucidating the molecular mechanisms of Na+ homeostasis and further functional identification of the BvNHX genes in sugar beet.

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“…Based on presence of the "ATDS" motif, a broader cation substrate specificity for Oryza NHX1 proteins is predicted. Thus, OsNHX1 shows broad substrate preference and suppresses Na + , Li + , and K + sensitivity of the yeast nhx1 mutant while O. coarctata (OcNHX1) suppresses Na + , but enhances Li + sensitivity of a yeast nhx1 mutant (Fukuda et al, 2004;Jegadeeson et al, 2019). Within the E 5 XXN 6 D 7 motif, E 5 is highly conserved in type I CPAs, with N 6 and D 7 being vital for ion binding and translocation.…”

Section: Discussionmentioning

confidence: 99%

Distinct Evolutionary Origins of Intron Retention Splicing Events in NHX1 Antiporter Transcripts Relate to Sequence Specific Distinctions in Oryza Species

Sellamuthu

Jegadeeson

et al. 2020

Front. Plant Sci.

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The genome of Asian cultivated rice (Oryza sativa L.) shows the presence of six organelle-specific and one plasma membrane (OsNHX1-7) NHX-type cation proton antiporters. Of these, vacuolar-localized OsNHX1 is extensively characterized. The genus Oryza consists of 27 species and 11 genome-types, with cultivated rice, diploid O. sativa, having an AA-type genome. Oryza NHX1 orthologous regions (gene organization, 5 upstream cis elements, amino acid residues/motifs) from closely related Oryza AA genomes cluster distinctly from NHX1 regions from more ancestral Oryza BB, FF and KKLL genomes. These sequence-specific distinctions also extend to two separate intron retention (IR) events involving Oryza NHX1 transcripts that occur at the 5 and 3 ends of the NHX1 transcripts. We demonstrate that the IR event involving the 5 UTR is present only in more recently evolved Oryza AA genomes while the IR event governing retention of the 13th intron of Oryza NHX1 (terminal intron) is more ancient in origin, also occurring in halophytic wild rice, Oryza coarctata (KKLL). We also report presence of a retro-copy of the OcNHX1 cDNA in the genome of O. coarctata (rOcNHX1). Preferential species and tissue specific up-or down-regulation of the correctly spliced NHX1 transcript/5 UTR/13th intron-retaining splice variants under salinity was observed. The implications of IR on NHX1 mRNA stability and ORF diversity in Oryza spp. is discussed.

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Expression of wild rice Porteresia coarctata PcNHX1 antiporter gene (PcNHX1) in tobacco controlled by PcNHX1 promoter (PcNHX1p) confers Na+-specific hypocotyl elongation and stem-specific Na+ accumulation in transgenic tobacco

Cited by 26 publications

References 33 publications

Genome-Wide Identification and Expression Analysis of the NHX (Sodium/Hydrogen Antiporter) Gene Family in Cotton

Genome-Wide Identification and Expression Analysis of the NHX (Sodium/Hydrogen Antiporter) Gene Family in Cotton

Genome-Wide Identification of Na+/H+ Antiporter (NHX) Genes in Sugar Beet (Beta vulgaris L.) and Their Regulated Expression under Salt Stress

Distinct Evolutionary Origins of Intron Retention Splicing Events in NHX1 Antiporter Transcripts Relate to Sequence Specific Distinctions in Oryza Species

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