Expression of xyloglucanase sp-Xeg gene from Penicillium canescens accelerates growth and rooting in transgenic aspen plants

Vidyagina, Elena O.; Kovalitskaya, Yu. A.; Loginov, Dmitry S.; Королева, О. В.; Shestibratov, Konstantin A.

doi:10.1134/s0003683814070060

Cited by 7 publications

(37 citation statements)

References 14 publications

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“…Since xyloglucan is involved in changing the cell shape in growth and differentiation zones [30], the expression of the xyloglucanase gene could also alter the wall extensibility in the leaf cells, which changed the shape of the cells and subsequently the leaf shape. Moreover, the observed in our study tendency of increasing the number of leaves in transgenic plants may have a compensatory effect on the growth characteristics of these plants [15]. xylan.…”

Section: Discussionsupporting

confidence: 47%

“…Xyloglucanase promotes cleavage of xyloglucan, thereby affecting the carbohydrate composition. A decrease in the content of pentosans (the main component of hemicellulose) was observed in all greenhouse plants [15]. In plants under semi-natural conditions, there was also a decrease in the content of pentosans in all transgenic lines in the youngest parts of the stem in comparison with control (Table 4).…”

Section: Carbohydrate Composition Of Xylemmentioning

confidence: 85%

“…In the initial study of the isoprene synthase gene ISPS 29 transgenic Grey poplar (Populus•× canescens) lines were generated [21], but only one line was used in the further semi-natural studies [22]. Therefore, following the common practice in the analysis of transgenic woody plants, six from 25 transgenic greenhouse lines generated in our previous study [15] were selected for this study under semi-natural conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The increase of height is considered as the main indicator of rapid growth in many similar studies related to selection for the faster growing plants [11,12,18,24]. Measurements of their heights demonstrated that not all of the previously identified promising lines [15] have maintained a higher plant height. Convergence of phenotypic traits between transgenic and control lines was observed.…”

Section: Discussionmentioning

confidence: 99%

“…Earlier, we noted that in a greenhouse environment, some transgenic lines showed an increase in the growth rate [15]. Transgenic and control plants of aspen were transferred from the greenhouse conditions to an open air and grown under semi-natural conditions up to the age of 18 months.…”

Section: Growth Ratementioning

confidence: 99%

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Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

Vidyagina

Subbotina²,

Белый³

et al. 2020

Preprint

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Background: Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to by a number of problems. Therefore, additional research is needed to alleviate them. Results: Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt. Conclusion: A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more markedly were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness and a decrease in the rate of decomposition of wood were observed.

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Section: Discussionsupporting

confidence: 47%

Section: Carbohydrate Composition Of Xylemmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Growth Ratementioning

confidence: 99%

See 3 more Smart Citations

Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

Vidyagina

Subbotina²,

Белый³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

et al. 2020

View full text Add to dashboard Cite

Background Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to a number of problems. Therefore, additional research is needed to alleviate them. Results Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt. Conclusion A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.

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Effects of the Аspen Short-Rotation Plantation on the C and N Biological Cycles in Boreal Forests: The Model Experiment

Komarov¹,

Komarov²

2015

Math.Biol.Bioinf.

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Аннотация. В статье проанализированы результаты модельного эксперимента по оценке экологических эффектов создания лесных плантаций на основе трансгенных форм осины. Система имитационных моделей EFIMOD, объединяющая балансовый и популяционный подходы, использована для анализа трансформации биологического круговорота углерода и азота при смене естественных лесных экосистем осиновыми плантациями (в т.ч. на основе деревьев с измененными параметрами роста и повышенным содержанием азота в органах). Модельный эксперимент выполнен на примере почвенно-климатических условий ельников северо-запада Ленинградской области. Моделировался рост плантаций осины с коротким оборотом рубки (30 лет) на месте вырубленных еловых лесов. Полученные результаты свидетельствуют о сокращении не только запасов органического вещества в лесных почвах, но и азота, несмотря на использование азотных минеральных удобрений. Эти потери объясняются выносом элементов почвенного питания быстро растущими древостоями как трансгенных, так и нетрансгенных форм осины, который не компенсируется возвратом элементов с ежегодным растительным опадом.Ключевые слова: моделирование биологического круговорота, система моделей EFIMOD, плантации трансгенных форм осины, имитационные сценарии, запасы углерода и азота.

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Expression of xyloglucanase sp-Xeg gene from Penicillium canescens accelerates growth and rooting in transgenic aspen plants

Cited by 7 publications

References 14 publications

Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

Effects of the Аspen Short-Rotation Plantation on the C and N Biological Cycles in Boreal Forests: The Model Experiment

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