Expression of zinc finger protein 105 in the testis and its role in male fertility

Zhou, Huaxin; Liu, LanHsin; Zhang, Heng; Lei, Zhenmin; Lan, Zi-Jian

doi:10.1002/mrd.21171

Cited by 9 publications

(15 citation statements)

References 33 publications

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“…Recently, studies demonstrated that several zinc finger proteins including ZNF230 (13), ZNF105 (14) and ZNF300 (15) were expressed specifically in testis and closely related to spermatogenesis and male reproduction. ZNF185 also belongs to the zinc finger protein family.…”

Section: Discussionmentioning

confidence: 99%

“…Zinc finger protein is one kind of transcription factor, which is widely existed in organisms, and plays an important role in cell proliferation, cell differentiation and cell apoptosis (9)(10)(11)(12). At present, several studies demonstrated that zinc finger protein was expressed specifically in testis, such as ZNF230 (13), ZNF105 (14) and ZNF300 (15), and closely associated with spermatogenesis and male reproduction. ZNF185, also known as ZFP185, is belonging to the ZNF family and identified by Heiss for the first time in 1997 (16).…”

Section: Introductionmentioning

confidence: 99%

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Expression pattern of Zinc finger protein 185 in mouse testis and its role in regulation of testosterone secretion

You

Fan

et al. 2017

Molecular Medicine Reports

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Zinc finger protein 185 (ZNF185) belongs to the ZNF family and is involved in cell proliferation and differentiation. To the best of our knowledge, the association between ZNF185 and male reproduction is unknown. In the present study, the expression and localization of ZNF185 in mouse testis, as well as its role in testosterone secretion, cell cycle progression and apoptosis of mouse Leydig cells were investigated. The results of the immunofluorescence analysis indicated that ZNF185 was highly expressed in Leydig cells of the mouse testis, and primarily localized in the cytoplasm. The results of quantitative polymerase chain reaction and western blot analyses further validated that ZNF185 expression was significantly higher in Leydig cells and sperm compared with that in Sertoli cells. Subsequently, the expression pattern of ZNF185 in mouse testis was determined at different developmental stages. The results demonstrated that the expression of ZNF185 was highest in the testis of 10‑week‑old mice and lowest in 2‑week‑old mice. Furthermore, the role of ZNF185 in Leydig cells of the mouse testis was investigated. Different concentrations of luteinizing hormone (LH) were used to stimulate the Leydig cells and subsequently the expression of ZNF185, and testosterone concentration was detected. The results revealed that LH upregulated the expression of ZNF185 and testosterone secretion, and ZNF185 expression was significantly positively correlated with testosterone secretion. To further validate whether ZNF185 was involved in testosterone secretion, lentiviral‑mediated RNA interference was used to knock down ZNF185 expression in Leydig cells. The results demonstrated that ZNF185 expression and testosterone secretion of Leydig cells were decreased significantly. In addition, the results demonstrated that the knockdown of ZNF185 expression did not significantly affect cell cycle progression or apoptosis. Taken together, the results of the present study revealed that ZNF185 was highly expressed in Leydig cells of the testis and involved in the secretion of testosterone. These results have contributed to the elucidation of the mechanism underlying male reproduction and may provide a novel target for the treatment of infertility, and the development of a contraceptive vaccine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression pattern of Zinc finger protein 185 in mouse testis and its role in regulation of testosterone secretion

You

Fan

et al. 2017

Molecular Medicine Reports

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“…Zinc finger proteins (ZNFs) consist of a series of molecules that have important biological functions . Recently, several studies have reported different zinc finger proteins, including ZNF185, ZNF230, ZNF105, and ZNF300, as being involved in spermatogenesis and male reproduction . Notably, ZNF185 belongs to the ZNF family and was identified by Heiss in 1997.…”

Section: Introductionmentioning

confidence: 99%

“…[4][5][6][7] Recently, several studies have reported different zinc finger proteins, including ZNF185, ZNF230, ZNF105, and ZNF300, as being involved in spermatogenesis and male reproduction. [8][9][10][11] Notably, ZNF185 belongs to the ZNF family and was identified by Heiss in 1997. It is located on the DXS52 region of Shutong Fan and Yuhan Zhao are co-first authors.…”

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confidence: 99%

ZNF185‐derived peptide induces fertility suppression in mice

Fan

Zhao

Pan

et al. 2018

Journal of Peptide Science

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Zinc finger protein 185 (ZNF185) belongs to the ZNF family and is involved in male reproduction. However, it is unclear whether ZNF185 may be a target candidate for contraceptive vaccines. In this study, antigenic peptides derived from ZNF185 were prepared, and their immune contraceptive effects were investigated using mice. Results from enzyme‐linked immunosorbent assay (ELISAs) showed that peptide immunization induced an antibody titre increase that reached a peak in week 12. Peptide‐3 and peptide‐4 were then chosen for subsequent experiments. The results of the fertility assays showed that peptide immunization inhibited the mating and fertility rates of the mice, whereas there were no obvious changes in the number of pups per litter. Subsequently, epididymal sperm was analysed. The results demonstrated that the sperm count and sperm motility were significantly decreased in the peptide group, while the amount of abnormal sperm was significantly increased in the peptide‐3 group. The male reproductive organs were also evaluated. There were no obvious differences in testis or epididymal weights, in the diameters of the seminiferous tubules, or in the thicknesses of the seminiferous epithelium between the peptide group and the phosphate buffer saline (PBS) group. In addition, histological analysis indicated that there were no obvious pathologic changes in testis and epididymal histology in the peptide group; however, the number of spermatozoa present in the epididymal lumen of the peptide group was significantly decreased when compared with the PBS group. Our study demonstrates for the first time that peptides derived from ZNF185 may induce fertility suppression in mice without damaging reproductive organs. These peptides have the potential to be used as a male contraceptive vaccine.

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“…Several Cys2/His2 zinc finger proteins are also expressed at specific stages during mouse spermatogenesis. For example, deficiency of ZFP105 in pachytene spermatocytes resulted in undifferentiated spermatogenic cells and decreased male fertility (Zhou et al 2010). Ovol2/MOVO may play an important role in the XY body during spermatogenesis, possibly in XY body formation and meiotic sex chromosome inactivation (Chizaki et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Abnormal spermatogenesis and male infertility in testicular zinc finger protein Zfp318‐knockout mice

et al. 2016

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Zfp318, a mouse gene with a Cys2/His2 zinc finger motif, is mainly expressed in germ cells in the testis. It encodes two alternative transcripts, which regulate androgen receptor-mediated transcriptional activation or repression by overexpression of them. However, the role of Zfp318 is still obscure in vivo, especially in spermatogenesis. To elucidate the role of Zfp318 during gamete production, we established a knockout mouse line. Zfp318-null male mice exhibited infertility, whereas Zfp318-null female mice displayed normal fertility. ZFP318 was expressed during multiple stages of spermatogenesis, from spermatocytes to round spermatids. The nuclei of secondary spermatocytes showed high levels of expression. Histological analysis and quantitative analysis of DNA content showed decreased numbers of both spermatids in the seminiferous tubules and mature spermatozoa in the epididymides of Zfp318-null mice. These results suggest that Zfp318 is expressed as a functional protein in testicular germ cells and plays an important role in meiosis during spermatogenesis.

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Expression of zinc finger protein 105 in the testis and its role in male fertility

Cited by 9 publications

References 33 publications

Expression pattern of Zinc finger protein 185 in mouse testis and its role in regulation of testosterone secretion

Expression pattern of Zinc finger protein 185 in mouse testis and its role in regulation of testosterone secretion

ZNF185‐derived peptide induces fertility suppression in mice

Abnormal spermatogenesis and male infertility in testicular zinc finger protein Zfp318‐knockout mice

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