Expression of ZNF396 in basal cell carcinoma

SHanK-associated rH domain-interacting protein (SHarPin) is a component of the linear ubiquitin chain assembly complex that can enhance the nF-κB and JnK signaling pathways, acting as a tumor-associated protein in a variety of cancer types. The present study investigated the role of SHarPin in cutaneous basal cell carcinoma (Bcc). Human Bcc (n=26) and normal skin (n=5) tissues, and Bcc (Te354.T) and normal skin (HacaT) cell lines were used to evaluate SHarPin expression level using immunohistochemistry and western blotting, respectively. a lentivirus carrying SHarPin-targeting or negative control short hairpin rna was infected into Te354.T cells, and the infected stable cells were assayed to analyze tumor cell proliferation, cell cycle, apoptosis, migration and invasion by cell counting Kit-8 and 5-ethynyl-2'-deoxyuridine incorporation assays, flow cytometry and Transwell assays. Western blotting was performed to assess the protein expression levels of gene signaling in SHarPin-silenced Bcc cells. SHarPin protein expression levels were downregulated or absent in Bcc cancer nests and precancerous lesions compared with normal skin samples. in addition, SHarPin expression levels were lower in Te354.T cells compared with HacaT cells. SHarPin shrna enhanced tumor cell proliferation and the S phase of the cell cycle, whereas Bcc cell apoptotic rates, and migratory and invasive abilities were not significantly altered. The expression levels of cyclin d1, cyclin-dependent kinase 4, phosphorylated-c-Jun and GLI family zinc finger 2 proteins were increased, whereas Patched 1 (PTcH1) and PTcH2 were decreased in the SHarPin-shrna-infected Bcc cells. Therefore, the present results suggested that SHarPin may act as a tumor suppressor during Bcc development. SHARPIN regulates cell proliferation of cutaneous basal cell carcinoma via inactivation of the transcriptional factors GLI2 and c-JUN

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“…Cell lines and culture. The human Bcc Te354.T cell line (40,41) was purchased from the american Tissue culture collection (cat. no.…”

Section: Methodsmentioning

confidence: 99%

SHARPIN regulates cell proliferation of cutaneous basal cell carcinoma via inactivation of the transcriptional factors GLI2 and c‑JUN

et al. 2020

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“…Tissues were immunostained with antibodies using the ImmPRESS polymerized reporter enzyme staining system (Vector Laboratories, Burlingame, CA, USA), as previously reported (Bai et al, 2014) and CSAII Biotin-free Tyramide Signal Amplification System (Dako, Japan).…”

Section: Methodsmentioning

confidence: 99%

A unique transgenic mouse model exhibiting a myeloproliferative disease-like phenotype

et al. 2019

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Transmembrane protein 207 (TMEM207) is an important molecule involved in invasiveness of gastric signet ring cell carcinoma. To understand the pathobiological effects of TMEM207, we generated thirteen transgenic mouse lines, designated C57BL/6-Tg (ITF-TMEM207), where mouse TMEM207 is expressed heterotrophically, regulated by the proximal promoter of the murine intestinal trefoil factor (ITF) gene (also known as Tff3). A C57BL/6-Tg (ITF-TMEM207) mouse line unexpectedly exhibited a high incidence of a spontaneous condition resembling myeloproliferative disease-like phenotype. Increased numbers of CD117+ cells and appearance of dysplastic myeloid cells in bone marrow were observed. These histopathological features suggested human myeloproliferative disease or its precursor manifestations, and were found in almost all mice within 1 year. TMEM207 immunoreactivity was identified in megakaryocytes and erythroblasts of the transgenic mice. The ITF-TMEM207 construct was inserted into Atg4b on murine chromosome 1. Myeloproliferative disease was not observed in other C57BL/6-Tg (ITF-TMEM207) transgenic mouse lines. However, although several other genetically manipulated animal models of myeloproliferative disease and Atg4b knockout mice exist, this mouse line harboring a mutated Atg4b gene, and with overexpression of TMEM207 protein, has not been reported as a model of myeloproliferative disease to date. The present study demonstrated that the C57BL/6-Tg (ITF-TMEM207) mouse may be a valuable model for improved understanding of human myeloproliferative disease.

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“…03-610102, Waltham, MA), respectively. Tissues were immunostained with antibodies using the ImmPRESS™ polymerized reporter enzyme staining system (Vector Laboratories, Burlingame, CA), as previously reported ( Bai et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Transgenic mouse model of cutaneous adnexal tumors

Kito

Saigo

Kurabayashi³

et al. 2014

Disease Models &Amp; Mechanisms

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TMEM207 was first characterized as being an important molecule for the invasion activity of gastric signet-ring cell carcinoma cells. In order to unravel the pathological properties of TMEM207, we generated several transgenic mouse lines, designated C57BL/6-Tg (ITF-TMEM207), in which murine TMEM207 was ectopically expressed under a truncated (by ~200 bp) proximal promoter of the murine intestinal trefoil factor (ITF) gene (also known as Tff3). Unexpectedly, a C57BL/6-Tg (ITF-TMEM207) mouse line exhibited a high incidence of spontaneous intradermal tumors with histopathological features that resembled those of various human cutaneous adnexal tumors. These tumors were found in ~14% female and 13% of male 6- to 12-month-old mice. TMEM207 immunoreactivity was found in hair follicle bulge cells in non-tumorous skin, as well as in cutaneous adnexal tumors of the transgenic mouse. The ITF-TMEM207 construct in this line appeared to be inserted to a major satellite repeat sequence at chromosome 2, in which no definite coding molecule was found. In addition, we also observed cutaneous adnexal tumors in three other C57BL/6-Tg (ITF-TMEM207) transgenic mouse lines. We believe that the C57BL/6-Tg (ITF-TMEM207) mouse might be a useful model to understand human cutaneous adnexal tumors.

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Expression of ZNF396 in basal cell carcinoma

Cited by 5 publications

References 20 publications

SHARPIN regulates cell proliferation of cutaneous basal cell carcinoma via inactivation of the transcriptional factors GLI2 and c‑JUN

SHARPIN regulates cell proliferation of cutaneous basal cell carcinoma via inactivation of the transcriptional factors GLI2 and c‑JUN

A unique transgenic mouse model exhibiting a myeloproliferative disease-like phenotype

Transgenic mouse model of cutaneous adnexal tumors

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