Expression of α-taxilin in hepatocellular carcinoma correlates with growth activity and malignant potential of the tumor

Abstract

Abstract. The membrane traffic system has been recognized to be involved in carcinogenesis and tumor progression in several types of tumors. ·-taxilin is a newly identified membrane traffic-related molecule, and its up-regulation has been reported in embryonic and malignant tissues of neural origin. In the present study, we analyzed the expression of ·-taxilin in relation to clinicopathological features of hepatocellular carcinomas (HCC) and proliferative activity of the tumor determined by proliferating cell nuclear antigen labeling index (PCNA-LI). Twenty-nine surgically resected nodules of HCC (8 well-, 11 moderately-, and 10 poorlydifferentiated) were studied. Fifteen cases showed 'strong staining', while 14 cases showed 'weak staining' for ·-taxilin. A significantly higher expression of ·-taxilin was observed in less-differentiated (p=0.005), and more invasive (p=0.016) HCCs. The 'strong staining' group showed significantly higher PCNA-LI than the 'weak staining' group (the medians of PCNA-LI were 59.4% vs. 14.4%, p<0.0001). We also evaluated the expression of ·-taxilin in hepatoma cell lines (PLC/PRF/5, Hep G2 and HuH-6) in association with cell proliferation. The expression levels of ·-taxilin protein were correlated with their growth rates. In conclusion, the expression of the ·-taxilin protein was related with an increased proliferative activity and a less-differentiated histological grade of HCC. ·-taxilin may be involved in cell proliferation of HCC, and its expression can be a marker of malignant potential of HCC.

Efficacy and safety of pyrotinib combined with albumin‐bound paclitaxel as first‐line treatment for HER2‐positive metastatic breast cancer in patients previously treated with adjuvant and/or neoadjuvant trastuzumab therapy: The stage 1 results of a single‐arm, phase 2 prospective clinical trial

“…33,34 Alpha-taxilin (TXLNA) has been reported in renal cell cancer, hepatocellular cancer, and pancreatic cancer. [35][36][37] To our knowledge, no study has found an association between TXLNA and breast cancer. Nectin-4 (PVRL4) is expressed in cancer tissues such as breast cancer.…”

“…Studies have found that CEA levels were affected by drugs such as trastuzumab and paclitaxel 33,34 . Alpha‐taxilin (TXLNA) has been reported in renal cell cancer, hepatocellular cancer, and pancreatic cancer 35–37 . To our knowledge, no study has found an association between TXLNA and breast cancer.…”

Expression of TXLNA in brain gliomas and its clinical significance: a bioinformatics analysis

“…TXLNA, for example, is a cancer suppressor gene in pancreatic cancer, yet it is a promoter of tumors in hepatocellular carcinoma and renal cell carcinoma, thus inducing their emergence and development. It was proposed by Natsuko Ohtomo et al that the TXLNA expression is linked to the augmented proliferation and poorly differentiated histological evaluation of hepatocellular carcinoma [ 7 ]. Tomoko Mashidori 1 et al found that the metastatic and invasive capacity of renal cell carcinoma was revealed to be augmented by a high expression of TXLNA [ 11 ].…”

“…As we all know, vesicular transport is involved in multiple processes of eukaryotic cell proliferation, and its function also suggests that this gene may be involved in tumor expression [ 6 ]. More than that, a few Studies have established TXLNA’s existence and manifestation in hepatocellular carcinoma, renal cell carcinoma, and pancreatic tumor cells [ 7 , 8 ]. In addition, Sueli M et al conducted real-time fluorescence quantitative PCR analysis of TXLNA genes using different grade astrocytic tumor samples in an experiment, confirming that their expression was higher compared to non-tumor CNS tissues [ 9 ].…”

Metallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma

“…Taxilin is involved in Ca 2+ -dependent exocytosis in neuroendocrine cells 63 . Its expression is correlated with growth activity and malignant potential of hepatocellular carcinoma 64 . It was previously shown that inhibiting lysosomal exocytosis reversed invasiveness and chemoresistance in aggressive sarcoma cells 65 , which highlights the possible role of taxilin as a key-player in MT3-mediated CDDP trafficking and resistance in Nbl cells.…”