Expression of α1-adrenoceptor subtypes in rat tissues: implications for α1-adrenoceptor classification

Noradrenaline contractions of human prostate mediated by α1c‐(α1c‐) adrenoceptor subtype

“…The affinity of a range of competitive antagonists has now been measured on membranes from cells transfected with the cDNA for each of the three cloned al subtypes in several studies. While prazosin has been found to be non-selective, others have shown some degree of selectivity between the subtypes (Faure et al, 1994;Forray et al, 1994b;Kenny et al, 1994a,b;Testa et al, 1994;Goetz et al, 1995). The affinity of the same antagonists has been measured here in functional studies for the al subtype mediating contraction of the prostate by calculation of pA2 values.…”

“…The responses to noradrenaline were not affected by the highest concentration Faure et al, 1994;Forray et al, 1994b;Kenny et al, 1994a,b;Testa et al, 1994;Goetz et al, 1995 (no s.e.mean for compounds with only one or two values). In each study the hamster alb, bovine aln and rat ald clones were used except for Goetz et al (1995) and Forray et al (1994b) where the three human al-subtype clones were used.…”

“…'Data from Marshall et al (1992). (Table 1) from Faure et al, 1994;Forray et al, 1994b;Kenny et al, 1994a,b;Testa et al, 1994;Goetz et al, 1995, with pA2 values for the antagonists prazosin (1), WB 4101 (2), 5-methyl urapidil (3), phentolamine (4), benoxathian (5), spiperone (6), indoramin (7) and BMY 7378 (8) against human prostate noradrenaline contractions. The solid line is a linear regression fit through all the points and the dashed line has a slope equal to unity, passing through the origin.…”

Expression of α1 adrenoceptor subtypes in rat corpus cavernosum

“…9 A variety of a1 adrenoceptor subtypes has been evidenced in rat tissues including genital organs as the vas deferens. 13 From experiments performed on human corpus cavernosum strips, it appears that at least two, maybe three adrenoceptor subtypes a1A, a1B and a1D are functionally expressed. 28 Therefore, both from an experimental point of view, proposing the rat as a useful model for study of erection, and with clinical perspectives in view of using this rat model for the study of male erectile dysfunction, we went further on in the investigation of a1 adrenoceptors equipment in the rat penis.…”

“…Furthermore a1 adrenoceptor subtypes has been evidenced in rat vas deferens. 13 A major advance in the treatment of urogenital dysfunctions is the identi®cation and pharmacological characterization of adrenoceptor subtypes equipment of the different urogenital organs. So, intracavernosal injections of a1 blocking agents which elicited tumescence or penile rigidity in man opened new perspectives in the clinical treatment of impotence.…”