Expression of β-amyloid precursor protein (APP) in human dorsal root ganglia

Naves, F.J.; Calzada, Begoña Calvo; Cabal, Álvaro Arias; Alonso-Cortina, V.; Soto, Miguel; Fernández-Sánchez, Maria Teresa; Vega, J.A.

doi:10.1016/0304-3940(94)90563-0

Cited by 12 publications

(3 citation statements)

References 22 publications

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“…A/β is processed in the Golgi complex and extracellular accumulation of A/β could impair the neighboring neurons, leading to their death (LaFerla et al, 1996). One of the derivatives of A/β is the abnormal metabolism of Alzheimer precursor protein A4 (APP, also named amyloid precursor protein) (Naves et al, 1994). APP is an integral membrane protein that is present on the plasma membrane of both neurons and glial cells in central nervous system (CNS).…”

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confidence: 99%

“…The extensive extracellular domain of APP is released from cells by proteolytic cleavage, forming various soluble fragments. There are at least five isoforms of APP (APP563, APP695, APP714, APP751, and APP770) derived by alternative splicing from a single gene; they all contain the β‐amyloid peptide amino acid sequence and three isoforms of them (APP563, APP751, and APP770) also have a Kunitz‐type serine protease inhibitor domain (Naves et al, 1994). The physiological functions of the secreted forms of APP have not been well characterized.…”

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Potential roles of Alzheimer precursor protein A4 and β‐amyloid in survival and function of aged spinal motor neurons after axonal injury

Xie

Chai

et al. 2003

J of Neuroscience Research

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To study the potential role of Alzheimer precursor protein A4 (APP) and beta-amyloid (A/beta) on aging motor neuron survival, expression of APP, A/beta, and choline acetyltransferase (ChaT) were investigated in aged rats after either distal axotomy or root avulsion injury. Approximately 45% in number of total aged spinal motor neuron were normally APP-positive. A/beta-positive neurites were observed normally in the spinal ventral horn of aged rats. After distal axotomy, without apparent neurodegeneration such as cell loss and decreased ChaT-immunoreactivity, increased levels of APP expression were observed in the spinal cords of aged rats post-injury. In contrast, after avulsion, expression of APP and A/beta were downregulated in the spinal ventral horn of aged rats, and marked loss of spinal motor neurons and downregulated ChaT expression were observed. Our data indicate that APP and A/beta might play beneficial roles in neuronal survival of aged spinal motor neurons after axonal injury.

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confidence: 99%

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confidence: 99%

Potential roles of Alzheimer precursor protein A4 and β‐amyloid in survival and function of aged spinal motor neurons after axonal injury

Xie

Chai

et al. 2003

J of Neuroscience Research

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“…The DCt was calculated by normalizing the mean Ct of each sample to the mean Ct of the reference gene measured in the same experimental conditions. For the quantification of each gene we considered a homogenous cell line (cultured Schwann cells) that constitutively expresses both NF1 and APP genes [18,19] as the positive sample (calibrator sample). The DDCt of each sample was then calculated by subtracting calibrator DCt to sample DCt.…”

Section: Measurement Of Nf1 and App Levels By Quantitative Real Time Pcrmentioning

confidence: 99%

Neurofibromin and Amyloid Precursor Protein Expression in Dopamine D3 Receptor Knock-Out Mice Brains

et al. 2010

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Recently, it has been proposed that neurofibromin (NF1) forms a binding complex with amyloid precursor protein (APP) that interacts with the dopamine D(3) receptor (D(3)R). In the present study we investigated whether the absence of the D(3)R is correlated to modifications in the expression of both NF1 and APP. Quantitative real-time PCR analyses of both transcripts showed that NF1 mRNA levels were significantly reduced whereas APP levels were strikingly increased in D(3)R knock-out (D(3)R KO) as compared to wild type (WT) mice brains. Western blot analyses using mice whole brains produced comparable results with those obtained by mRNA measurements. Moreover, immunohistochemical analyses revealed a similar brain regional distribution of APP protein in the hippocampus, in the cerebral and cerebellar cortex of D(3)R KO mice. Conversely, hippocampal NF1 immunoreactivity did not seem to be affected by the absence of D(3)Rs. Further analyses confirmed that regional NF1 protein expression in the hippocampus was not affected by the absence of the D(3)R, whereas APP levels were still increased in this specific brain region. In conclusion, these results show the existence of a correlation among the D(3)R, NF1 and APP in mice brains and thus show the regional-specific regulation of NF1 in brains of D(3)R KO, which may contribute to gain insights into the comprehension of novel underlying mechanisms that regulate brain function.

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S‐100 proteins in the human peripheral nervous system

González-Martínez

Pérez-Piñera

Díaz-Esnal

et al. 2003

Microscopy Res & Technique

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130

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This article reviews the distribution of S100 proteins in the human peripheral nervous system. The expression of S100 by peripheral glial cells seems to be a distinctive fact of these cells, independently of their localization and their ability to myelinate or not. S100 proteins expressing cells include satellite cells of sensory, sympathetic and enteric ganglia, supporting cells of the adrenal medulla, myelinating and non-myelinating Schwann cells in the nerve trunks, and the Schwann-related cells of sensory corpuscles. In addition, S100 proteins are expressed in peripheral neurons. Most of them express S100alpha protein, and a subpopulation of sensory neurons in dorsal root ganglia contains S100beta protein or S100alpha plus S100beta proteins.

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Expression of β-amyloid precursor protein (APP) in human dorsal root ganglia

Cited by 12 publications

References 22 publications

Potential roles of Alzheimer precursor protein A4 and β‐amyloid in survival and function of aged spinal motor neurons after axonal injury

Potential roles of Alzheimer precursor protein A4 and β‐amyloid in survival and function of aged spinal motor neurons after axonal injury

Neurofibromin and Amyloid Precursor Protein Expression in Dopamine D3 Receptor Knock-Out Mice Brains

S‐100 proteins in the human peripheral nervous system

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