Expression of β‐defensin‐1 in chimpanzee (<i>Pan troglodytes</i>) airways

Duits, L. A.; Langermans, Jan A. M.; Paltansing, Sunita; Straaten, Tahar van der; Vervenne, Richard A. W.; Frost, Patrice A.; Hiemstra, Pieter S.; Thomas, Alan W.; Nibbering, Peter H.

doi:10.1034/j.1600-0684.2000.290502.x

Cited by 6 publications

(5 citation statements)

References 19 publications

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“…Porcine monocytes do not express β‐defensins 27 whereas β‐defensin mRNA expression has been reported in bovine bone marrow cells 30 . We recently described the expression of β‐defensin‐1 mRNA in chimpanzee alveolar macrophages 31 . In the course of our study on β‐defensin expression in chimpanzee macrophages, expression of hBD‐1 mRNA in human alveolar macrophages was noted 31 .…”

Section: Introductionmentioning

confidence: 83%

“…We recently described the expression of β‐defensin‐1 mRNA in chimpanzee alveolar macrophages 31 . In the course of our study on β‐defensin expression in chimpanzee macrophages, expression of hBD‐1 mRNA in human alveolar macrophages was noted 31 . Because β‐defensins may play an important role in the interface between the innate and adaptive immune systems, we investigated in the present study the expression of hBD‐1 and hBD‐2 mRNA by blood monocytes, macrophages and (immature) DC.…”

Section: Introductionmentioning

confidence: 99%

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Expression of β‐defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells

Duits

Ravensbergen²,

Rademaker³

et al. 2002

Immunology

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233

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SUMMARYHuman b-defensins are broad-spectrum antimicrobial peptides known to be produced by epithelial cells. It was recently shown that b-defensins also display chemotactic activity for dendritic cells (DC) and T cells, and thus may serve to link innate and adaptive immunity. The aim of the present study was to explore expression of mRNA for these peptides in mononuclear phagocytes and DC. The results revealed that monocytes, monocyte-derivedmacrophages (MDM), and monocyte-derived-dendritic cells (DC) all express humanb-defensin-1 (hBD-1) mRNA. hBD-1 mRNA expression by monocytes and MDM was increased after activation with interferon-c (IFN-c) and/or lipopolysaccharide (LPS) in a dose-and time-dependent fashion. Alveolar macrophages showed an intense hBD-1 expression, which could not be further increased. Expression of hBD-1 mRNA by immature DC was low, and increased considerably after maturation. Monocytes, MDM, alveolar macrophages and DC showed a limited expression of human b-defensin-2 (hBD-2) mRNA, which could only be increased in monocytes and alveolar macrophages by IFN-c and/or LPS in a dose-and time-dependent fashion. Immunocytochemical stainings demonstrated the expression of hBD-2 peptide by freshly isolated blood monocytes and alveolar macrophages in cytospin preparations.

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Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Expression of β‐defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells

Duits

Ravensbergen²,

Rademaker³

et al. 2002

Immunology

Self Cite

233

157

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“…Of particular note in the latter study is that intratracheal instillation of IgG antibody to P. aeruginosa prevented the increase in respiratory epithelial permeability, whereas systemic perfusion of the same antibody protected the endothelial barrier, suggesting that the properties of the epithelial and endothelial barriers are independently regulated. Although lung airways (and type II cells) secrete defensins as a critical component of the host defense response against infectious organisms (1,34,60), the exact role of defensins in mediating alveolar epithelial permeability alterations is unknown.…”

Section: Lung Protein Clearance Under Pathological Conditionsmentioning

confidence: 99%

Protein transport across the lung epithelial barrier

Kim

Malik

2003

American Journal of Physiology-Lung Cellular and Molecular Phys

181

130

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. Because concentrations of plasma proteins in alveolar fluid can increase in injured lungs (such as with permeability edema and inflammation), understanding how alveolar epithelium handles protein transport is needed to develop therapeutic measures to restore alveolar homeostasis. This review provides an update on recent findings on protein transport across the alveolar epithelial barrier. The use of primary cultured rat alveolar epithelial cell monolayers (that exhibit phenotypic and morphological traits of in vivo alveolar epithelial type I cells) has shown that albumin and IgG are absorbed via saturable processes at rates greater than those predicted by passive diffusional mechanisms. In contrast, secretory component, the extracellular portion of the polymeric immunoglobulin receptor, is secreted into alveolar fluid. Transcytosis involving caveolae and clathrincoated pits is likely the main route of alveolar epithelial protein transport, although relative contributions of these internalization steps to overall protein handling of alveolar epithelium remain to be determined. The specific pathways and regulatory mechanisms responsible for translocation of proteins across lung alveolar epithelium and regulation of the cognate receptors (e.g., 60-kDa albumin binding protein and IgG binding FcRn) expressed in alveolar epithelium need to be elucidated. alveolar epithelial cells; albumin; secretory component; immunoglobulin G MACROMOLECULE TRANSPORT ACROSS alveolar epithelium has been investigated over the years, but little is known to date concerning the mechanisms and underlying pathways regulating transalveolar protein clearance. Alveolar protein clearance is fundamental to the resolution of the transudate and exudate after hydrostatic-and especially high permeability-type pulmonary edema. The inability to clear excess proteins from air spaces is associated with poor prognosis in patients with alveolar flooding pulmonary edema. Clearance of edema fluid via active ion transport processes raises the oncotic pressure that, in the absence of efficient clearance mechanisms for proteins, slows alveolar fluid clearance. Patients with acute respiratory distress syndrome (ARDS), for example, have large quantities of precipitated protein in their air spaces (3), and nonsurvivors have three times more protein in their air spaces than survivors (21). In addition, excess serum proteins in alveolar edema fluid may lead to precipitation and modification of proteins (e.g., by reactive oxygen and nitrogen species). Although macrophages may contribute to clearance of the excess proteins, the integrity of the alveolar epithelial barrier may not be readily re-

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“…1998), also express Pdefensins in cattle. In addition, P-defensins have been identified in humans Harder, 1997a), chimpanzees (Duits. 2000), rhesus monkeys (Bals.…”

Section: P-defensinsmentioning

confidence: 99%

“…Human Pdefensin-1 is probably not a homolog of TAP or LAP as it is not inducible, nor does its promoter region contain NF-KB binding regions , although the HBD-1 gene does contain NF IL-6 and INF-y consensus sites, suggesting possible influences on HBD-1 expression by inflammatory mediators (Valore, 1996;Liu, 1996). The chimpanzee also expresses a possible HBD-l homolog in airway and skin, termed chimpanzee p-defensin-1 (cBDl) (Duits, 2000).…”

Section: P-defensinsmentioning

confidence: 99%

The effect of Mannheimia haemolytica pneumonia on antimicrobial peptide expression in ruminant lung

Rae

McNeil²

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This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer.The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction.In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps.

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Expression of β‐defensin‐1 in chimpanzee (Pan troglodytes) airways

Cited by 6 publications

References 19 publications

Expression of β‐defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells

Expression of β‐defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells

Protein transport across the lung epithelial barrier

The effect of Mannheimia haemolytica pneumonia on antimicrobial peptide expression in ruminant lung

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