Expression of β-nerve growth factor and homeobox A10 in experimental cryptorchidism treated with exogenous nerve growth factor

Xian, Hua; Xian, Yun; Liu, Huan; Wang, Yongjun; He, Jianghong; Huang, Jianfei

doi:10.3892/mmr.2014.3005

Cited by 3 publications

(3 citation statements)

References 24 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Epithelial ovarian cancer (EOC) is a major cause for malignancy-associated female mortality ( 1 ). The mortality rate is frequently increased by a delay in diagnosis and drug resistance ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10

et al. 2018

View full text Add to dashboard Cite

Ovarian cancer is the most lethal gynecological cancer and its metastasis leads to a poor prognosis. The present study was designed to elucidate how microRNA (miR)-665 regulates the proliferation and migration of ovarian tumor cells. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that miR-665 expression was decreased in ovarian cancer tissues. Increased expression of miR-665 suppressed the growth and migration of ovarian cancer cells, whereas the downregulated expression of miR-665 led to the opposite results. Bioinformatics tools identified homeobox A10 (HOXA10) as a target of miR-665. Following miR-665 overexpression, HOXA10 protein expression was significantly reduced. A dual luciferase assay revealed that miR-665 bound to the 3′-untranslated region of HOXA10. Immunohistochemistry and RT-PCR revealed that the expression of HOXA10 was negatively correlated with the expression of miR-665. It was concluded that miR-665 targets HOXA10 and may act as a tumor-suppressing gene in ovarian cancer. This pathway may be involved in the development and metastasis of ovarian cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Satokata et al., (1995) ascertained that being localized solely to the gubernacular region of the male reproductive system, HOX10, when absent, caused cryptorchidism. Similarly, Xian et al., (2015) determined that, in rats, HOXA10 was localized to the germ, interstitial and Leydig cells of the testes and expressed at a lower level in cryptorchid testes. In another study conducted in mice by Bomgardner et al., (2003), it was found that HOXA10 was expressed in all regions of the epididymis and deferens.…”

Section: Discussionmentioning

confidence: 86%

Immunolocalization of some HOX proteins in immature and mature feline testes

Topaloğlu

Akbalık

Sağsöz

2021

Anat Histol Embryol

View full text Add to dashboard Cite

Homeobox (HOX) proteins are known for their critical role in body shape formation and tissue differentiation of developing vertebrate embryos. Recent research has shown that HOX proteins have many physiological roles such as cell proliferation, cell cycle, apoptosis and cell differentiation in adults, as well as the development of the vertebrate nerve and reproductive system. This study was conducted to determine the possible physiological functions and expression intensities of HOXA10, HOXA11, HOXB6 and HOXC6 proteins in the male reproductive system (testes, epididymis and deferens ducts), which are important for the continuity of some specific cat breeds in different age ranges. In the study, a total of 18 testicular tissues were used, divided into two groups: less than 6 months (immature) and more than 1 year (mature). Tissue samples were then subjected to immunohistochemical staining with protein‐specific antibodies examined in the study. In the findings obtained in the research; it was observed that HOXA10, HOXA11, HOXB6 and HOXC6 produced different intensities of immunolocalization in the epididymis and ductus deferens layers in the immature and mature testicular cells. In addition, it was found that HOXA10 immunoreaction was also seen in some vascular endothelial cells. As a result, it was concluded that the HOX proteins could contribute to the physiological functions of testes, epididymis and ductus deferens and affect male fertility.

show abstract

“…To identify potential TFs driving conserved alternative promoter use in the three species, FIMO ( Grant et al, 2011 ) was used to predict the locations of TF-binding motifs in the MLT1F1 sequences, revealing a core “GTCATAAAA” sequence shared by all three species, predicted to bind to 14 TFs (Supplementary Figure S8). Among these TFs, HOX proteins are crucial for testicular and epididymal physiological functions and can affect male fertility ( Ferguson & Agoulnik, 2013 ; Topaloğlu et al, 2021 ; Xian et al, 2015 ). Species-conserved TE-derived alternative promoters were not limited to the same subfamily of TEs, i.e., different TE subfamilies acted on the same gene across the three species.…”

Section: Resultsmentioning

confidence: 99%

Pig H3K4me3, H3K27ac, and gene expression profiles reveal reproductive tissue-specific activity of transposable elements

Jiang,

Zhou,

Ling

et al. 2024

Zoological Research

View full text Add to dashboard Cite

Regulatory sequences and transposable elements (TEs) account for a large proportion of the genomic sequences of species; however, their roles in gene transcription, especially tissue-specific expression, remain largely unknown. Pigs serve as an excellent animal model for studying genomic sequence biology due to the extensive diversity among their wild and domesticated populations. Here, we conducted an integrated analysis using H3K27ac ChIP-seq, H3K4me3 ChIP-seq, and RNA-seq data from 10 different tissues of seven fetuses and eight closely related adult pigs. We aimed to annotate the regulatory elements and TEs to elucidate their associations with histone modifications and mRNA expression across different tissues and developmental stages. Based on correlation analysis between mRNA expression and H3K27ac and H3K4me3 peak activity, results indicated that H3K27ac exhibited stronger associations with gene expression than H3K4me3. Furthermore, 1.45% of TEs overlapped with either the H3K27ac or H3K4me3 peaks, with the majority displaying tissue-specific activity. Notably, a TE subfamily (LTR4C_SS), containing binding motifs for SIX1 and SIX4, showed specific enrichment in the H3K27ac peaks of the adult and fetal ovaries. RNA-seq analysis also revealed widespread expression of TEs in the exons or promoters of genes, including 4 688 TE-containing transcripts with distinct development stage-specific and tissue-specific expression. Of note, 1 967 TE-containing transcripts were enriched in the testes. We identified a long terminal repeat (LTR), MLT1F1, acting as a testis-specific alternative promoter in SRPK2 (a cell cycle-related protein kinase) in our pig dataset. This element was also conserved in humans and mice, suggesting either an ancient integration of TEs in genes specifically expressed in the testes or parallel evolutionary patterns. Collectively, our findings demonstrate that TEs are deeply embedded in the genome and exhibit important tissue-specific biological functions, particularly in the reproductive organs.

show abstract

Expression of β-nerve growth factor and homeobox A10 in experimental cryptorchidism treated with exogenous nerve growth factor

Cited by 3 publications

References 24 publications

MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10

MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10

Immunolocalization of some HOX proteins in immature and mature feline testes

Pig H3K4me3, H3K27ac, and gene expression profiles reveal reproductive tissue-specific activity of transposable elements

Contact Info

Product

Resources

About